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Stem Cells and Lineage Commitment, 1st ed. 2024 Methods and Protocols Methods in Molecular Biology Series, Vol. 2736

Langue : Anglais

Coordonnateur : Turksen Kursad

Couverture de l’ouvrage Stem Cells and Lineage Commitment
This detailed volume explores a variety of techniques used to study lineage commitment in stem cells. Further elucidation of the process that stem cells undergo on their way to becoming more specified cell types is vital for a more complete understanding of cell biology and overall physiology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Stem Cells and Lineage Commitment: Methods and Protocols serves as an ideal guide for experts and novices in the field of stem cell biology.

1. Lineage Tracing by Single-Cell Transcriptomics Decoding Dynamics of Lineage Commitment

            Ping Yu and Lin Cheng

 

2. Cleavage Under Targets & Release Using Nuclease (CUT&RUN) of Histone Modifications in Epidermal Stem Cells of Adult Murine Skin

            Pooja Flora and Elena Ezhkova

 

3. Targeted Gene Silencing by Using GapmeRs in Differentiating Human Induced Pluripotent Stem Cells (hiPSC) Towards Pancreatic Progenitors

            Lucas Unger, Luiza Ghila, and Simona Chera

 

4. Retinoic Acid-Mediated Differentiation of Mouse Embryonic Stem Cells to Neuronal Cells

            Sangeeta Dutta, Debosree Pal, and M.R.S. Rao

 

5. Isolation and Functional Analysis of Myoepithelial Cells from Adult Mouse Submandibular Glands

            Rika Yasuhara, Seya Kang, Rino Tokumasu, and Kenji Mishima

 

6. Chromosomal Analysis in Lineage-Specific Mouse Hematopoietic Stem Cells and Progenitors

            Nur Afizah Yusoff, Zariyantey Abd Hamid, Paik Wah Chow, Salwati Shuib, Izatus Shima Taib, and Siti Balkis Budin

 

7. Easy and Rapid Methods for Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells and Human Umbilical Wharton’s Jelly-Derived Mesenchymal Stem Cells

            Figen Abatay Sel, Ayse Erol, Mediha Suleymanoglu, Durdane Serap Kuruca, and Fatma Savran Oguz

 

8. Encapsulation of MSCs in PRP-Derived Fibrin Microbeads

            Özge Lalegül-Ülker, Şükran Şeker, Ayşe Eser Elçin, and Yaşar Murat Elçin

 

9. Assessing Neuronogenic-vs-Astrogenic Bias of Neural Stem Cells via In Vitro Clonal Assay

            Laura Rigoldi and Antonello Mallamaci

 

10. Stem Cell-Based Modeling Protocol for Parkinson's Disease

            Babak Arjmand, Shayesteh Kokabi Hamidpour, Hamid Reza Aghayan, Sepideh Alavi-Moghadam, Rasta Arjmand, Mostafa Rezaei-Tavirani, Parisa Goodarzi, Ensieh Nasli‐Esfahani, and Mohsen Nikandish

 

11. Standard Operating Procedure for Production of Mouse Brown Adipose Tissue-Derived Mesenchymal Stem Cells

            Babak Arjmand, Mostafa Rezaei-Tavirani, Sepideh Alavi-Moghadam, Akram Tayanloo-Beik, Mahdi Gholami, Shayesteh Kokabi Hamidpour, Rasta Arjmand, Ahmad Rezazadeh Mafi, Fereshteh Mohamadi-jahani, and Bagher Larijani

 

12. Development and Validation of Type 2 Diabetic Zebrafish Model for Cell-Based Treatments

            Babak Arjmand, Sepideh Alavi-Moghadam, Shayesteh Kokabi Hamidpour, Rasta Arjmand, Mostafa Rezaei-Tavirani, Bagher Larijani, Parisa Goodarzi, Neda Mehrdad, and Mohsen Rajaeinejad

 

13. Neuromuscular Junction-on-a-Chip for Amyotrophic Lateral Sclerosis Modeling

            Sepideh Alavi-Moghadam, Shayesteh Kokabi Hamidpour, Mostafa Rezaei-Tavirani, Bagher Larijani, Rasta Arjmand, Fakher Rahim, Ahmad Rezazadeh Mafi, Hossein Adibi, and Babak Arjmand

 

14. Primary Human Leukemia Stem Cell (LSC) Isolation and Characterization

            Neslihan Meriç and Fatih Kocabaş

 

15. GMP-Compliant Mesenchymal Stem Cell-Derived Exosomes for Cell Free Therapy in Cancer

            Babak Arjmand, Sepideh Alavi-Moghadam, Mostafa Rezaei-Tavirani, Shayesteh Kokabi Hamidpour, Rasta Arjmand, Kambiz Gilany, Mohsen Rajaeinejad, Fakher Rahim, Nazli Namazi, and Bagher Larijani

 

16. Establishing Brain Tumor Stem Cell Culture from Patient Brain Tumors and Imaging Analysis of Patient-Derived Xenografts

            Elham Mahmnoudian and Arezu Jahani-Asl

 

17. An Optimized Protocol for PiggyBac-Induced iPSC Generation from hPBMCs by Automatic Electroporation

            Pelin Kilic and Begum Cosar

 

18. Signalling Pathways in Trans-Differentiation of Mesenchymal Stem Cells: Recent Advances

            Vaishak Kaviarasan, Dikshita Deka, Darshini Balaji, Surajit Pathak, and Antara Banerjee

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts

Date de parution :

Ouvrage de 226 p.

17.8x25.4 cm

Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).

168,79 €

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