Embryonic Stem Cell Immunobiology, Softcover reprint of the original 1st ed. 2013 Methods and Protocols Methods in Molecular Biology Series, Vol. 1029

Bone marrow stem cells are the most transplanted cells worldwide. These cells are used as a replacement therapy for patients suffering from a diverse number of hematopoietic diseases and immunodeficiencies.  However, the use of bone marrow cells in regenerative medicine has so far remained without much success. In the new era of pluripotent stem cells, great opportunities for establishing new therapies have opened up. The discovery of human embryonic stem cells and that of induced pluripotent (iPS) stem cells has made it possible to derive any desired tissues for regenerative medicine as iPS cell derived cells are only limited by the lack of established protocols that can be applied in humans. There is no doubt that stem cells present a new and innovative platform for establishing novel cell based therapies. The challenge is to establish new protocols that allow the successful differentiation of these cells into lineage committed cells. Embryonic Stem Cell Immunobiology: Methods and Protocols covers a variety of relevant topics, such as hematopoietic stem cells derived from ES cells, the interaction of these cells with natural killer cells or with cytotoxic T cells, and specific protocols for the derivation of hematopoietic cells and neuronal cells, to name a few. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Embryonic Stem Cell Immunobiology: Methods and Protocols serves as an ideal guide to experts and non-experts interested in different aspects of stem cells.

1. Immune Privilege of Stem Cells

Naoki Ichiryu and Paul J. Fairchild

2. Immunogenicity of In vitro Maintained and Matured Populations: Potential Barriers to Engraftment of Human Pluripotent Stem Cell Derivatives

Chad Tang, Irving L. Weissman, and Micha Drukker

3. Hematopoietic and Natural Killer Cell Development from Human Pluripotent Stem Cells

Zhenya Ni, David A. Knorr, and Dan S. Kaufman

4. Evaluation of Immunogenicity of Rat ES-Cell Derived Endothelial Cells

Martin Seifert and Juliane Ladhoff

5. Interaction of ES Cell Derived Neural Progenitor Cells with Natural Killer Cells and Cytotoxic T Cells

Casimir de Rham and Jean Villard

6. Strategies to Generate Induced Pluripotent Stem Cells

Michael Hayes and Nicholas Zavazava

7. The Differentiation and Lineage Commitment of Murine Embryonic Stem Cells into Insulin Producing Cells

Sudhanshu P. Raikwar and Nicholas Zavazava

8. Mouse ES Cell-Derived Hematopoietic Progenitor Cells

Eun-Mi Kim and Nicholas Zavazava

9. Directed Differentiation of Embryonic Stem Cells to the T-Lymphocyte Lineage

Chen Yao Liang, Roxanne Holmes, and Juan Carlos Zuniga-Pflucker

10. Development of Hematopoietic Stem and Progenitor Cells from Mouse Embryonic Stem Cells, in vitro, Supported by Ectopic Human HOXB4 Expression

Hannes Klump

11. Histone Modification Profiling in Normal and Transformed Human Embryonic Stem Cells Using Micro Chromatin Immunoprecipitation, Scalable to Genome-Wide Microarray Analyses

Angelique Schnerch, Sharavanti Rampalii, and Mickie Bhatia

12. Combined Total Proteomic and Phosphoproteomic Analysis of Human Pluripotent Stem Cells

Junjie Hou, Brian T. D. Tobe, Frederick Lo, Justin D. Blethrow, Andrew M. Crain, Dieter A. Wolf, Evan Y. Snyder, Ilyas Singec, and Laurence M. Brill

13. Transcriptional Regulatory Mechanisms that Govern Embryonic Stem Cell Fate

Styabrata Das and Dana Levasseur

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts