Liver Stem Cells, Softcover reprint of the original 1st ed. 2012 Methods and Protocols Methods in Molecular Biology Series, Vol. 826

Increasing evidence suggests that liver stem cells have the capacity to differentiate into parenchymal hepatocytes or into bile ductular cells. These stem cells may be activated to proliferate after severe liver injury or exposure to hepatocarcinogens. Stem cell replacement strategies are being investigated as an alternative approach to liver repair and regeneration. Additionally, stem cell transplantation has been shown to significantly improve liver function and increase survival in experimentally-induced liver-injury models in animals. In Liver Stem Cells: Methods and Protocols, expert researchers focus on  several hepatic progenitor cells, hepatic differentiation form stem cells, bile ductal cell formation from stem cells, liver stem cells and hepatocarcinogenesi, and  application of liver stem cells for cell therapy.  These topics shed light on stem cell technology which may lead to the development of effective clinical modalities for human liver diseases. Written in the highly successful Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Thorough and intuitive, Liver Stem Cells: Methods and Protocols seeks to aid scientists in the further study of preclinical and clinical investigations that explore the therapeutic potential of stem cells in repair of liver injuries.

Contents

Part I Several hepatic progenitor cells:

1)      Purification and Culture of Fetal Mouse Hepatoblasts that are Precursors of Mature Hepatocytes and Biliary Epithelial Cells

          Nobuyoshi Shiojiri and Miho Nitou

2)      Clinical Uses of Liver Stem Cells

Yock Young Dan

3)      Identification and Isolation of Adult Liver Stem/Progenitor Cells

Minoru Tanaka and Atsushi Miyajima

4)      Isolation and Purification Method of Mouse Fetal Hepatoblasts

Luc Gailhouste

5)      Isolation of Hepatic Progenitor Cells from the Galactosamine-Treated Rat Liver

Norihisa Ichinohe, Junko Kon, Toshihiro Mitaka

II. Hepatic differentiation form stem cells:

6)      Purification of Adipose Tissue Mesenchymal Stem cells (AT-MSC) and Differentiation Towards Hepatic-Like Cells

Agnieszka Banas

7) Development of Immortalized Hepatocyte-like Cell from hMSC

Adisak Wongkajornsilp, Khanit Sa-ngiamsuntorn, , Suradej Hongeng,

8) Isolation of Adult Human Pluripotent Stem Cells from Mesenchymal Cell Populations and their Application to Liver Damages

Shohei Wakao, Masaaki Kitada, Yasumasa Kuroda, Mari Dezawa

9) Generation and Hepatic Differentiation of Human iPS Cells

Tetsuya Ishikawa, Keitaro Hagiwara, and Takahiro Ochiya

10) Efficient Hepatic Differentiation from Human iPS cells by Gene Transfer

Kenji Kawabata, Mitsuru Inamura & Hiroyuki Mizuguchi

11) “Tet-On” System Toward Hepatic Differentiation of Human Mesenchymal Stem Cells by Hepatocyte Nuclear Factor

Goshi Shiota, Yoko Yoshida, Noriko Matsumoto, Yoshiko Hoshikawa

12) SAMe and HuR in Liver Physiology Usefulness of Stem Cells in Hepatic Differentiation Research

Laura Gomez-Santos, Mercedes Vazquez-Chantada, Jose Maria Mato and Maria Luz Martinez-Chantar.

Part III BD formation from stem cells:

13) Transdifferentiation of Mature Hepatocytes into Bile Duct Cells within a Collagen Gel Matrix

Yuji Nishikawa

Part IV. Liver stem cells and hepatocarcinogenesis:

14) Identification of Cancer Stem Cell-Related MicroRNAs in Hepatocellular Carcinoma

Junfang Ji and Xin Wei Wang

Part V. Application of liver stem cells for cell therapy:

15) Intravenous Human Mesenchymal Stem Cells (MSC) Transplantation in NOD/SCID Mice Preserve Liver Integrity of Irradiation Damage

Moubarak Mouiseddine, Sabine François, Maâmar Souidi and Alain Chapel

16) Engineering of Implantable Liver Tissues

Y. Sakai, M. Nishikawa, F. Evenou, M. Hamon, H. Huang, K. P. Montagne, N. Kojima, T. Fujii and T. Niino

17) Mesenchymal Stem Cell Therapy on Murine Model of Non-Alcoholic Steatohepatitis

Yoshio Sakai, Shuichi Kaneko

3)      Identification and Isolation of Adult Liver Stem/Progenitor Cells

Minoru Tanaka and Atsushi Miyajima

4)      Isolation and Purification Method of Mouse Fetal Hepatoblasts

Luc Gailhouste

5)      Isolation of Hepatic Progenitor Cells from the Galactosamine-Treated Rat Liver

Norihisa Ichinohe, Junko Kon, Toshihiro Mitaka

II. Hepatic differentiation form stem cells:

6)      Purification of Adipose Tissue Mesenchymal Stem cells (AT-MSC) and Differentiation Towards Hepatic-Like Cells

Agnieszka Banas

7) Development of Immortalized Hepatocyte-like Cell from hMSC

Adisak Wongkajornsilp, Khanit Sa-ngiamsuntorn, , Suradej Hongeng,

8) Isolation of Adult Human Pluripotent Stem Cells from Mesenchymal Cell Populations and their Application to Liver Damages

Shohei Wakao, Masaaki Kitada, Yasumasa Kuroda, Mari Dezawa

9) Generation and Hepatic Differentiation of Human iPS Cells

Tetsuya Ishikawa, Keitaro Hagiwara, and Takahiro Ochiya

10) Efficient Hepatic Differentiation from Human iPS cells by Gene Transfer

Kenji Kawabata, Mitsuru Inamura & Hiroyuki Mizuguchi

11) “Tet-On” System Toward Hepatic Differentiation of Human Mesenchymal Stem Cells by Hepatocyte Nuclear Factor

Goshi Shiota, Yoko Yoshida, Noriko Matsumoto, Yoshiko Hoshikawa

12) SAMe and HuR in Liver Physiology Usefulness of Stem Cells in Hepatic Differentiation Research

Laura Gomez-Santos, Mercedes Vazquez-Chantada, Jose Maria Mato and Maria Luz Martinez-Chantar.

Part III BD formation from stem cells:

13) Transdifferentiation of Mature Hepatocytes into Bile Duct Cells within a Collagen Gel Matrix

Yuji Nishikawa

Part IV. Liver stem cells and hepatocarcinogenesis:

14) Identification of Cancer Stem Cell-Related MicroRNAs in Hepatocellular Carcinoma

Junfang Ji and Xin Wei Wang

Part V. Application of liver stem cells for cell therapy:

15) Intravenous Human Mesenchymal Stem Cells (MSC) Transplantation in NOD/SCID Mice Preserve Liver Integrity of Irradiation Damage

Moubarak Mouiseddine, Sabine François, Maâmar Souidi and Alain Chapel

16) Engineering of Implantable Liver Tissues

Y. Sakai, M. Nishikawa, F. Evenou, M. Hamon, H. Huang, K. P. Montagne, N. Kojima, T. Fujii and T. Niino

17) Mesenchymal Stem Cell Therapy on Murine Model of Non-Alcoholic Steatohepatitis

Yoshio Sakai, Shuichi Kaneko

8) Isolation of Adult Human Pluripotent Stem Cells from Mesenchymal Cell Populations and their Application to Liver Damages

Shohei Wakao, Masaaki Kitada, Yasumasa Kuroda, Mari Dezawa

9) Generation and Hepatic Differentiation of Human iPS Cells

Tetsuya Ishikawa, Keitaro Hagiwara, and Takahiro Ochiya

10) Efficient Hepatic Differentiation from Human iPS cells by Gene Transfer

Kenji Kawabata, Mitsuru Inamura & Hiroyuki Mizuguchi

11) “Tet-On” System Toward Hepatic Differentiation of Human Mesenchymal Stem Cells by Hepatocyte Nuclear Factor

Goshi Shiota, Yoko Yoshida, Noriko Matsumoto, Yoshiko Hoshikawa

12) SAMe and HuR in Liver Physiology Usefulness of Stem Cells in Hepatic Differentiation Research

Laura Gomez-Santos, Mercedes Vazquez-Chantada, Jose Maria Mato and Maria Luz Martinez-Chantar.

Part III BD formation from stem cells:

13) Transdifferentiation of Mature Hepatocytes into Bile Duct Cells within a Collagen Gel Matrix

Yuji Nishikawa

Part IV. Liver stem cells and hepatocarcinogenesis:

14) Identification of Cancer Stem Cell-Related MicroRNAs in Hepatocellular Carcinoma

Junfang Ji and Xin Wei Wang

Part V. Application of liver stem cells for cell therapy:

15) Intravenous Human Mesenchymal Stem Cells (MSC) Transplantation in NOD/SCID Mice Preserve Liver Integrity of Irradiation Damage

Moubarak Mouiseddine, Sabine François, Maâmar Souidi and Alain Chapel

16) Engineering of Implantable Liver Tissues

Y. Sakai, M. Nishikawa, F. Evenou, M. Hamon, H. Huang, K. P. Montagne, N. Kojima, T. Fujii and T. Niino

17) Mesenchymal Stem Cell Therapy on Murine Model of Non-Alcoholic Steatohepatitis

Yoshio Sakai, Shuichi Kaneko

11) “Tet-On” System Toward Hepatic Differentiation of Human Mesenchymal Stem Cells by Hepatocyte Nuclear Factor

Goshi Shiota, Yoko Yoshida, Noriko Matsumoto, Yoshiko Hoshikawa

12) SAMe and HuR in Liver Physiology Usefulness of Stem Cells in Hepatic Differentiation Research

Laura Gomez-Santos, Mercedes Vazquez-Chantada, Jose Maria Mato and Maria Luz Martinez-Chantar.

Part III BD formation from stem cells:

13) Transdifferentiation of Mature Hepatocytes into Bile Duct Cells within a Collagen Gel Matrix

Yuji Nishikawa

Part IV. Liver stem cells and hepatocarcinogenesis:

14) Identification of Cancer Stem Cell-Related MicroRNAs in Hepatocellular Carcinoma

Junfang Ji and Xin Wei Wang

Part V. Application of liver stem cells for cell therapy:

15) Intravenous Human Mesenchymal Stem Cells (MSC) Transplantation in NOD/SCID Mice Preserve Liver Integrity of Irradiation Damage

Moubarak Mouiseddine, Sabine François, Maâmar Souidi and Alain Chapel

16) Engineering of Implantable Liver Tissues

Y. Sakai, M. Nishikawa, F. Evenou, M. Hamon, H. Huang, K. P. Montagne, N. Kojima, T. Fujii and T. Niino

17) Mesenchymal Stem Cell Therapy on Murine Model of Non-Alcoholic Steatohepatitis

Yoshio Sakai, Shuichi Kaneko

3)      Identification and Isolation of Adult Liver Stem/Progenitor Cells

Minoru Tanaka and Atsushi Miyajima

4)      Isolation and Purification Method of Mouse Fetal Hepatoblasts

Luc Gailhouste

5)      Isolation of Hepatic Progenitor Cells from the Galactosamine-Treated Rat Liver

Norihisa Ichinohe, Junko Kon, Toshihiro Mitaka

II. Hepatic differentiation form stem cells:

6)      Purification of Adipose Tissue Mesenchymal Stem cells (AT-MSC) and Differentiation Towards Hepatic-Like Cells

Agnieszka Banas

7) Development of Immortalized Hepatocyte-like Cell from hMSC

Adisak Wongkajornsilp, Khanit Sa-ngiamsuntorn, , Suradej Hongeng,

8) Isolation of Adult Human Pluripotent Stem Cells from Mesenchymal Cell Populations and their Application to Liver Damages

Shohei Wakao, Masaaki Kitada, Yasumasa Kuroda, Mari Dezawa

9) Generation and Hepatic Differentiation of Human iPS Cells

Tetsuya Ishikawa, Keitaro Hagiwara, and Takahiro Ochiya

10) Efficient Hepatic Differentiation from Human iPS cells by Gene Transfer

Kenji Kawabata, Mitsuru Inamura & Hiroyuki Mizuguchi

11) “Tet-On” System Toward Hepatic Differentiation of Human Mesenchymal Stem Cells by Hepatocyte Nuclear Factor

Goshi Shiota, Yoko Yoshida, Noriko Matsumoto, Yoshiko Hoshikawa

12) SAMe and HuR in Liver Physiology Usefulness of Stem Cells in Hepatic Differentiation Research

Laura Gomez-Santos, Mercedes Vazquez-Chantada, Jose Maria Mato and Maria Luz Martinez-Chantar.

Part III BD formation from stem cells:

13) Transdifferentiation of Mature Hepatocytes into Bile Duct Cells within a Collagen Gel Matrix

Yuji Nishikawa

Part IV. Liver stem cells and hepatocarcinogenesis:

14) Identification of Cancer Stem Cell-Related MicroRNAs in Hepatocellular Carcinoma

Junfang Ji and Xin Wei Wang

Part V. Application of liver stem cells for cell therapy:

15) Intravenous Human Mesenchymal Stem Cells (MSC) Transplantation in NOD/SCID Mice Preserve Liver Integrity of Irradiation Damage

Moubarak Mouiseddine, Sabine François, Maâmar Souidi and Alain Chapel

16) Engineering of Implantable Liver Tissues

Y. Sakai, M. Nishikawa, F. Evenou, M. Hamon, H. Huang, K. P. Montagne, N. Kojima, T. Fujii and T. Niino

17) Mesenchymal Stem Cell Therapy on Murine Model of Non-Alcoholic Steatohepatitis

Yoshio Sakai, Shuichi Kaneko

8) Isolation of Adult Human Pluripotent Stem Cells from Mesenchymal Cell Populations and their Application to Liver Damages

Shohei Wakao, Masaaki Kitada, Yasumasa Kuroda, Mari Dezawa

9) Generation and Hepatic Differentiation of Human iPS Cells

Tetsuya Ishikawa, Keitaro Hagiwara, and Takahiro Ochiya

10) Efficient Hepatic Differentiation from Human iPS cells by Gene Transfer

Kenji Kawabata, Mitsuru Inamura & Hiroyuki Mizuguchi

11) “Tet-On” System Toward Hepatic Differentiation of Human Mesenchymal Stem Cells by Hepatocyte Nuclear Factor

Goshi Shiota, Yoko Yoshida, Noriko Matsumoto, Yoshiko Hoshikawa

12) SAMe and HuR in Liver Physiology Usefulness of Stem Cells in Hepatic Differentiation Research

Laura Gomez-Santos, Mercedes Vazquez-Chantada, Jose Maria Mato and Maria Luz Martinez-Chantar.

Part III BD formation from stem cells:

13) Transdifferentiation of Mature Hepatocytes into Bile Duct Cells within a Collagen Gel Matrix

Yuji Nishikawa

Part IV. Liver stem cells and hepatocarcinogenesis:

14) Identification of Cancer Stem Cell-Related MicroRNAs in Hepatocellular Carcinoma

Junfang Ji and Xin Wei Wang

Part V. Application of liver stem cells for cell therapy:

15) Intravenous Human Mesenchymal Stem Cells (MSC) Transplantation in NOD/SCID Mice Preserve Liver Integrity of Irradiation Damage

Moubarak Mouiseddine, Sabine François, Maâmar Souidi and Alain Chapel

16) Engineering of Implantable Liver Tissues

Y. Sakai, M. Nishikawa, F. Evenou, M. Hamon, H. Huang, K. P. Montagne, N. Kojima, T. Fujii and T. Niino

17) Mesenchymal Stem Cell Therapy on Murine Model of Non-Alcoholic Steatohepatitis

Yoshio Sakai, Shuichi Kaneko

11) “Tet-On” System Toward Hepatic Differentiation of Human Mesenchymal Stem Cells by Hepatocyte Nuclear Factor

Goshi Shiota, Yoko Yoshida, Noriko Matsumoto, Yoshiko Hoshikawa

12) SAMe and HuR in Liver Physiology Usefulness of Stem Cells in Hepatic Differentiation Research

Laura Gomez-Santos, Mercedes Vazquez-Chantada, Jose Maria Mato and Maria Luz Martinez-Chantar.

Part III BD formation from stem cells:

13) Transdifferentiation of Mature Hepatocytes into Bile Duct Cells within a Collagen Gel Matrix

Yuji Nishikawa

Part IV. Liver stem cells and hepatocarcinogenesis:

14) Identification of Cancer Stem Cell-Related MicroRNAs in Hepatocellular Carcinoma

Junfang Ji and Xin Wei Wang

Part V. Application of liver stem cells for cell therapy:

15) Intravenous Human Mesenchymal Stem Cells (MSC) Transplantation in NOD/SCID Mice Preserve Liver Integrity of Irradiation Damage

Moubarak Mouiseddine, Sabine François, Maâmar Souidi and Alain Chapel

16) Engineering of Implantable Liver Tissues

Y. Sakai, M. Nishikawa, F. Evenou, M. Hamon, H. Huang, K. P. Montagne, N. Kojima, T. Fujii and T. Niino

17) Mesenchymal Stem Cell Therapy on Murine Model of Non-Alcoholic Steatohepatitis

Yoshio Sakai, Shuichi Kaneko

8) Isolation of Adult Human Pluripotent Stem Cells from Mesenchymal Cell Populations and their Application to Liver Damages

Shohei Wakao, Masaaki Kitada, Yasumasa Kuroda, Mari Dezawa

9) Generation and Hepatic Differentiation of Human iPS Cells

Tetsuya Ishikawa, Keitaro Hagiwara, and Takahiro Ochiya

10) Efficient Hepatic Differentiation from Human iPS cells by Gene Transfer

Kenji Kawabata, Mitsuru Inamura & Hiroyuki Mizuguchi

11) “Tet-On” System Toward Hepatic Differentiation of Human Mesenchymal Stem Cells by Hepatocyte Nuclear Factor

Goshi Shiota, Yoko Yoshida, Noriko Matsumoto, Yoshiko Hoshikawa

12) SAMe and HuR in Liver Physiology Usefulness of Stem Cells in Hepatic Differentiation Research

Laura Gomez-Santos, Mercedes Vazquez-Chantada, Jose Maria Mato and Maria Luz Martinez-Chantar.

Part III BD formation from stem cells:

13) Transdifferentiation of Mature Hepatocytes into Bile Duct Cells within a Collagen Gel Matrix

Yuji Nishikawa

Part IV. Liver stem cells and hepatocarcinogenesis:

14) Identification of Cancer Stem Cell-Related MicroRNAs in Hepatocellular Carcinoma

Junfang Ji and Xin Wei Wang

Part V. Application of liver stem cells for cell therapy:

15) Intravenous Human Mesenchymal Stem Cells (MSC) Transplantation in NOD/SCID Mice Preserve Liver Integrity of Irradiation Damage

Moubarak Mouiseddine, Sabine François, Maâmar Souidi and Alain Chapel

16) Engineering of Implantable Liver Tissues

Y. Sakai, M. Nishikawa, F. Evenou, M. Hamon, H. Huang, K. P. Montagne, N. Kojima, T. Fujii and T. Niino

17) Mesenchymal Stem Cell Therapy on Murine Model of Non-Alcoholic Steatohepatitis

Yoshio Sakai, Shuichi Kaneko

11) “Tet-On” System Toward Hepatic Differentiation of Human Mesenchymal Stem Cells by Hepatocyte Nuclear Factor

Goshi Shiota, Yoko Yoshida, Noriko Matsumoto, Yoshiko Hoshikawa

12) SAMe and HuR in Liver Physiology Usefulness of Stem Cells in Hepatic Differentiation Research

Laura Gomez-Santos, Mercedes Vazquez-Chantada, Jose Maria Mato and Maria Luz Martinez-Chantar.

Part III BD formation from stem cells:

13) Transdifferentiation of Mature Hepatocytes into Bile Duct Cells within a Collagen Gel Matrix

Yuji Nishikawa

Part IV. Liver stem cells and hepatocarcinogenesis:

14) Identification of Cancer Stem Cell-Related MicroRNAs in Hepatocellular Carcinoma

Junfang Ji and Xin Wei Wang

Part V. Application of liver stem cells for cell therapy:

15) Intravenous Human Mesenchymal Stem Cells (MSC) Transplantation in NOD/SCID Mice Preserve Liver Integrity of Irradiation Damage

Moubarak Mouiseddine, Sabine François, Maâmar Souidi and Alain Chapel

16) Engineering of Implantable Liver Tissues

Y. Sakai, M. Nishikawa, F. Evenou, M. Hamon, H. Huang, K. P. Montagne, N. Kojima, T. Fujii and T. Niino

17) Mesenchymal Stem Cell Therapy on Murine Model of Non-Alcoholic Steatohepatitis

Yoshio Sakai, Shuichi Kaneko

Includes topics that shed light on stem cell technology, leading to the development of effective clinical modalities for human liver diseases

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts