Skin Stem Cells (2^nd Ed., 2nd ed. 2019) Methods and Protocols Methods in Molecular Biology Series, Vol. 1879

1. In Vivo Genetic Alteration and Lineage Tracing of Single Stem Cells by Live Imaging

            Olivia Farrelly, Paola Kuri, and Panteleimon Rompolas

2. Ex Vivo Imaging and Genetic Manipulation of Mouse Hair Follicle Bulge Stem Cells

            Daniel Haensel, Melissa A. McNeil, and Xing Dai

3. Quantitative Detection of Low-Abundance Transcripts at Single-Cell Level in Human Epidermal Keratinocytes by Digital Droplet Reverse Transcription-Polymerase Chain Reaction

            Frédéric Auvré, Julien Coutier, Michèle T. Martin, and Nicolas O. Fortunel

4. Qualitatively Monitoring Binding and Expression of the Transcription Factors Sp1 and NFI as a Useful Tool to Evaluate the Quality of Primary Cultured Epithelial Stem Cells in Tissue Reconstruction

            Gaëtan Le-Bel, Sergio Cortez Ghio, Danielle Larouche, Lucie Germain, and Sylvain L. Guérin

5. Ribonucleoproteins Mediated Efficient In Vivo Gene Editing in Skin Stem Cells

            Wenbo Wu and Ting Chen

6. Isolation and Characterization of Cutaneous Epithelial Stem Cells

            Stephanie R. Gillespie and David M. Owens

7. Interfollicular Epidermal Stem Cells: Boosting and Rescuing from Adult Skin

            Mariana T. Cerqueira, Ana M. Frias, Rui L. Reis, and Alexandra P. Marques

8. Whole-Mount Immunofluorescent Staining Coupled to Multicolour Lineage Tracing Model for Analyzing the Spatio-Temporal Organization of Epidermal Stem Cells

            Edwige Roy and Kiarash Khosrotehrani

9. Isolation and Enrichment of Newborn and Adult Skin Stem Cells of the Interfollicular Epidermis

            Stefano Sol, Dario Antonini, and Caterina Missero

10. Isolation and Cultivation of Epidermal (Stem) Cells

            Xusheng Wang, Shiyang Dong, and Yaojiong Wu

11. One-Step Simple Isolation Method to Obtain Both Epidermal and Dermal Stem Cells from Human Skin Specimen

12. Isolation and Cultivation of Skin-Derived Precursors

            Xiaoxiao Wang, Shiyang Dong, and Yaojiong Wu

13. Magnetic-Based Cell Isolation Technique for the Selection of Stem Cells

            Petek Korkusuz, Sevil Köse, Nilgün Yersal, and Selin Önen

14. Isolation of Human Skin Epidermal Stem Cells Based on the Expression of Endothelial Protein C Receptor

            Meilang Xue, Suat Dervish, and Christopher J. Jackson

15. Decellularized bSIS-ECM as a Regenerative Biomaterial for Skin Wound Repair

16. Protocols for Full Thickness Skin Wound Repair Using Prevascularized Human Mesenchymal Stem Cell Sheet

            Lei Chen, Daniel Radke, Shaohai Qi, and Feng Zhao

17. Cultivation of Adipose-Derived Stromal Cells on Intact Amniotic Membrane-Based Scaffold for Skin Tissue Engineering

            Ehsan Taghiabadi, Bahareh Beiki, Nasser Aghdami, and Amir Bajouri

18. Amniotic Membrane Seeded Fetal Fibroblasts as Skin Substitute for Wound Regeneration

            Ehsan Taghiabadi, Bahareh Beiki, Nasser Aghdami, and Amir Bajouri

19. Skin Wound Healing: Refractory Wounds and Novel Solutions

            Gabriel M. Virador, Lola de Marcos, and Victoria M. Virador

20. Isolation, Culture, and Motility Measurements of Epidermal Melanocytes from GFP-Expressing Reporter Mice

            Lina Dagnino and Melissa Crawford

21. Melanoblasts as Multipotent Cells in Murine Skin

            Tsutomu Motohashi and Takahiro Kunisada

22. Regeneration of Mouse Skin Melanocyte Stem Cells In Vivo and In Vitro

            Ke Yang, Weiming Qiu, Pei-Rong Gu, and Mingxing Lei

23. Interactions between Epidermal Keratinocytes, Dendritic Epidermal T-Cells, and Hair Follicle Stem Cells

            Krithika Badarinath, Abhik Dutta, Akshay Hegde, Neha Pincha, Rupali Gund, and Colin Jamora

24. Isolating Immune Cells from Mouse Embryonic Skin

            Ambika S. Kurbet and Srikala Raghavan

25. Direct Conversion of Mouse Embryonic Fibroblasts into Neural Crest Cells

            Tsutomu Motohashi and Takahiro Kunisada

26. High-Titer Production of HIV-Based Lentiviral Vectors in Roller Bottles for Gene and Cell Therapy

            Hazal Banu Olgun, Hale Tasyurek, Ahter Dilsad Sanlioglu, and Salih Sanlioglu

27. High Grade Purification of Third-Generation HIV-Based Lentiviral Vectors by Anion Exchange Chromatography for Experimental Gene and Stem Cell Therapy Applications

            Hazal Banu Olgun, Hale Tasyurek, Ahter D. Sanlioglu, and Salih Sanlioglu

28. Full-Thickness Human Skin Equivalent Models of Atopic Dermatitis

            Gopu Sriram, Paul Lorenz Bigliardi, and Mei Bigliardi-Qi

29. Human Hair Follicle Associated-Pluripotent (hHAP) Stem Cells Differentiate to Cardiac Muscle Cells

            Robert M. Hoffman

30. Isolation of Normal Fibroblasts and Their Cancer-Associated Counterparts (CAFs) for Biomedical Research

            Barbora Dvořánková, Lukáš Lacina, and Karel Smetana Jr.

31. Isolation of Cancer Stem Cells from Squamous Cell Carcinoma

            Silvia Fontenete and Mirna Perez-Moreno

32. Identification of Human Cutaneous Squamous Cell Carcinoma Cancer Stem Cells

            Carlotta Olivero, Huw Morgan, and Girish K. Patel

33. Identification of Human Cutaneous Basal Cell Carcinoma Cancer Stem Cells

            Huw Morgan, Carlotta Olivero, and Girish K. Patel

Includes fully revised and all new cutting-edge methods and protocols for the study of skin stem cell research

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts