Skin Stem Cells, 2013 Methods and Protocols Methods in Molecular Biology Series, Vol. 989

During the last decade, an increased interest in somatic stem cells has led to a flurry of research on one of the most accessible tissues of the body: skin.  Much effort has focused on such topics as understanding the heterogeneity of stem cell pools within the epidermis and dermis, and their comparative utility in regenerative medicine applications.  In Skin Stem Cells: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study skin stem cells. These include methods and techniques for the isolation, maintenance and characterization of stem cell populations from skin. Written in the highly successful Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 Authoritative and practical, Skin Stem Cells: Methods and Protocols seeks to aid scientists in the further understanding of these diverse cell types and the translation of their biological potential to the in vivo setting.

1. Interfollicular Epidermal Stem Cells: Boosting and Rescuing from Adult Skin

M. T. Cerqueira, A. M. Frias, R. L. Reis, A. P. Marques

2. Isolation and Cultivation of Human Scalp Interfollicular Epidermal Stem Cells

Longmei Zhao, Basil M. Hantash

3. Isolation of Hair Follicle Bulge Stem Cells From YFP-Expressing Reporting Mice

Kerry-Ann Nakrieko, Timothy S. Irvine and Lina Dagnino

4. ­­­Analysis of Bulge Stem Cells from the Epidermis using Flow Cytometry

Rehan M. Villani, Mehmet Deniz Akyuz, Michaela T. Niessen, Carien M. Niessen

5. Lineage Tracing of Hair Follicle Stem Cells in Epidermal Whole Mounts

Monika Petersson, Daniela Frances, and Catherin Niemann

6. Isolation and Characterization of Cutaneous Epithelial Stem Cells

Uffe B. Jensen, Soosan Ghazizadeh, and David M. Owens

7. Identification and Analysis of Epidermal Stem Cells from Primary Mouse Keratinocytes

Youliang Wang and Xiao Yang

8. Monitoring the Cycling Activity of Cultured Human Keratinocytes using a CFSE-based Dye Tracking Approach

Loubna Chadli, Emmanuelle Cadio, Pierre Vaigot, Michèle T. Martin and Nicolas O. Fortunel

9. Quantification of Cyclobutane Pyrimidine Dimers in Human Epidermal Stem Cells

Ruetze M , Gallinat S , Wenck H ,Knott A

10. Analysis of Gene Expression in Skin using Laser Capture Microdissection

Briana Lee, Mikhail Geyfman, Bogi Andersen and Xing Dai

11. Qualitatively Monitoring Binding and Expression of the Transcription Factor Sp1 as a Useful Tool to Evaluate the Reliability of Primary Cultured Epithelial Stem Cells in Tissue Reconstruction

Manon Gaudreault, Danielle Larouche, Lucie Germain, and Sylvain L. Guérin

12. Genetic Manipulation of Keratinocyte Stem Cells with Lentiviral Vectors

Masahito Yasuda, David J. Claypool, Erika Guevara, Dennis R. Roop and Jiang Chen

13. Using 3D Culture to Investigate the Role of Mechanical Signalling in Keratinocyte Stem Cells

Lee Wallace and Julia Reichelt

14. In vivo Transplantation Assay at Limiting Dilution to Identify the Intrinsic Tissue Reconstitutive Capacity of Keratinocyte Stem Cells and their Progeny

Holger Schlüter and Pritinder Kaur

15. Melanoblasts as Multipotent Cells in Murine Skin

Tsutomu Motohashi and Takahiro Kunisada

16. Generation of Human Melanocytes from Induced Pluripotent Stem Cells

Shigeki Ohta, Yoichi Imaizumi, Wado Akamatsu, Hideyuki Okano, and Yutaka Kawakami

17. Cellular Populations Isolated from Newborn Mouse Skin Including Mesenchymal Stem Cells

Lauren Kimlin and Victoria Virador

18. Isolation, Characterization and Differentiation of Human Multipotent Dermal Stem Cells

Ling Li, Mizuho Fukunaga-Kalabis and Meenhard Herlyn

19. Isolation and Differentiation of Hair Follicle-derived Dermal Precursors

Andrew Hagner and Jeff Biernaskie

20. Isolation of Mesenchymal Stem Cells from Human Dermis

Tsutomu Soma, Jiro Kishimoto, and David Fisher

21. Skin Derived Mesenchymal Stem Cells: Isolation, Culture and Characterization

M. Orciani and R. Di Primio

Karl Gledhill, Aaron Gardner and Colin A. B. Jahoda

23. Enrichment of Oral Mucosa and Skin Keratinocyte Progenitor/Stem Cells

Kenji Izumi

24. Skin Stem Cells

Keita Inoue and Kotaro Yoshimura

Includes cutting-edge methods and protocols in the MiMB standard format

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras