Hepatic Stem Cells, 1st ed. 2019 Methods and Protocols Methods in Molecular Biology Series, Vol. 1905

Langue : Anglais

Coordonnateur : Tanimizu Naoki

Couverture de l’ouvrage Hepatic Stem Cells

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This volume looks at the liver?s epithelial cells?hepatocytes and cholangiocytes?and their progenitors. This book is divided into five parts: isolation of progenitor cells; characterization of liver progenitors in vivo; generation of hepatocytes, cholangiocytes, and their progenitors; reconstitution of liver tissue structures; and liver injury models. The chapters in this book cover topics such as expansion of bipotential liver stem/progenitor cells (LPCs) from fetal and neonatal liver; identifying progenitor cells involved in liver regeneration in vivo; methods for generating hepatocytes and cholangiocytes from multiple cellular sources; 3D tissue structures ex vivo; and resolving hepatic fibrosis by bone marrow transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Hepatic Stem Cells: Methods and Protocols is a valuable resource to help researchers understand the current theories used to study hepatic stem/progenitor cells, and aid them in performing experiments related to liver biology and pathophysiology.

Long-Term Culture of Mouse Fetal Hepatic Stem/Progenitor Cells.- Isolation of Bipotential Liver Progenitor Cells from Neonatal Mouse Liver.- Identification and Isolation of Clonogenic Cholangiocyte in Mouse.- Isolation and Expansion of Rat Hepatocytic Progenitor Cells.- Genetic Lineage Tracing of Biliary Epithelial Cells.- Specific Labeling and Lineage Tracing of Periportal Hepatocytes Using Two-Step Genetic Recombination.- Analysis for the Heterogeneity of Liver Progenitor Cells.- Chemical Screening Using a Zebrafish Model for Liver Progenitor Cell-Driven Liver Regeneration.- Conversion of Fibroblasts to Hepatocytes In Vitro.- Conversion of Fibroblasts to Hepatocyte-Like Cells In Vivo.- Chemically Induced Liver Progenitors (CLiPs): A Novel Cell Source for Hepatocytes and Biliary Epithelial Cells.- Induction of Functional Hepatocytes from Human iPSCs.- Culture System of Bile Duct-Like Cystic Structures Derived from HumanInducible Pluripotent Stem Cells.- Generation of Hepatic Tissue Structures Using Multicellular Spheroid Culture.- Reconstruction of Hepatic Tissue Structures Using Interstitial Flow in a Microfluidic Device.- Generation of Hepatic Organoids with Biliary Structures.- Analysis for Remodeling Hepatic Tissue Structures in 3D during Regeneration.- Canine Liver Fibrosis Model to Assess the Functions of Infused Autologous Bone Marrow-Derived Cells.- A Rodent Model for Cell Transplantation of Hepatic Progenitor Cells.- Mouse Model for Hepatocellular Carcinoma and Cholangiocarcinoma Originated from Mature Hepatocytes.- Mouse Model for Cholangiocarcinoma from Peribiliary Glands.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts