Induced Pluripotent Stem (iPS) Cells (2^nd Ed., 2nd ed. 2022) Methods and Protocols Methods in Molecular Biology Series, Vol. 2454

1. Human Induced Pluripotent Stem Cell (iPSC) Handling Protocols: Maintenance, Expansion, and Cryopreservation

            Davide Marotta, Chandrika Rao, and Valentina Fossati

2. Generation of Induced Pluripotent Stem Cells from Human Bone Marrow-Derived Mesenchymal Stem Cells

3. Analysis of Clonal Composition in Human iPSC and ESC and Derived 2D and 3D Differentiated Cultures

            Bernat del Olmo, Daria Merkurjev, Likun Yao, Mel·lina Pinsach-Abuin, Ivan Garcia-Bassets, and Angels Almenar-Queralt

4. Culturing Human Pluripotent Stem Cells on Micropatterned Silicon Surfaces

            Varvara Chalmantzi, Chara Simitzi, Angelos Papadopoulos, Eleni Bagli, Carol Murphy, Emmanuel Stratakis, and Theodore Fotsis

5. Porcine iPSC Generation: Testing Different Protocols to a Successful Application

            Oriol Iborra-Egea, Daina Martínez-Falguera, Santiago Roura, Antoni Bayes-Genis, Ángel Raya, and Carolina Gálvez-Montón

6. Efficient High-Density hiPSCs Expansion in Simple Dialysis Device

            Fuad Gandhi Torizal, Hyunjin Choi, Marie Shinohara, and Yasuyuki Sakai

7. Expanding the Differentiation Potential of Already-Established Pluripotent Stem Cells

            José González-Martínez and Marcos Malumbres

8. Generation of Quiescent Cardiac Fibroblasts Derived from Human Induced Pluripotent Stem Cells

            Hao Zhang, Mengcheng Shen, and Joseph C. Wu

9. Inductive Co-Culture Differentiation of Induced Pluripotent Stem Cells into Cardiomyocytes

            Axel J. Chu and Chinten James Lim

10. 3D Microwell Platform for Cardiomyocyte Differentiation of Human Pluripotent Stem Cells

            Mariana A. Branco, Tiago P. Dias, João P. Cotovio, Carlos A.V. Rodrigues, Tiago G. Fernandes, Joaquim M.S. Cabral, and Maria Margarida Diogo

11. Scalable Generation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

            Sarkawt Hamad, Daniel Derichsweiler, Jürgen Hescheler, and Kurt Pfannkuche

12. Application of Human Induced Pluripotent Stem Cell Technology for Cardiovascular Regenerative Pharmacology

            Qasim A. Majid, Barbara Orsolits, Lotta Pohjolainen, Zsófia Kovács, Gábor Földes, and Virpi Talman

13. Efficient and Safe Method of Generating Induced Pluripotent Stem Cells from Human Skin Fibroblasts and Subsequent Differentiation into Functional Cardiomyocytes

            Sheeja Rajasingh, Vinoth Sigamani, Narasimman Gurusamy, and Johnson Rajasingh

14. An Optical-Flow-Based Method to Quantify Dynamic Behavior of Human Pluripotent Stem Cell-Derived Cardiomyocytes in Disease Modeling Platforms

            Mohammad Izadifar, Tünde Berecz, Ágota Apáti, and Andras Nagy

15. Production of Innervated Skeletal Muscle Fibers Using Human Induced Pluripotent Stem Cells

            Mégane Delourme, Natacha Broucqsault, Kilian Mazaleyrat, and Frédérique Magdinier

16. Generation of Human Neural Progenitors from Blood Samples by Interrupted Reprogramming

            Eszter Szabó, Flóra Juhász, László Homolya, Zsuzsa Erdei, and Ágota Apáti

17. Robust and Highly Efficient Protocol for Differentiation of Human Pluripotent Stem Cells into Mesenchymal Stem Cells

            Manale Karam and Essam M. Abdelalim

18. The Development of Tissue Engineering Scaffolds Using Matrix from iPS-Reprogrammed Fibroblasts

            Francesco Santarella, Fergal J. O’Brien, Jonathan A. Garlick, and Cathal J. Kearney

19. 3D Organoid Culture Using Skin Keratinocytes Derived from Human Induced Pluripotent Stem Cells

            Tomoko Miyake and Mikio Shimada

20. Pluripotent Stem Cell Differentiation towards Functional Basal Stratified Epithelial Cells

            Eduardo Soares and Huiqing Zhou

21. Serum-Free Production of Three-Dimensional Hepatospheres from Pluripotent Stem Cells

            Hassan Rashidi and David C. Hay

22. The Differentiation of Human Induced Pluripotent Stem Cells into PodocytesIn Vitro

            Andrea F. Wise, Sheetal Saini, and Sharon D. Ricardo

23. A Method for Encapsulation and Transplantation into Diabetic Mice of Human Induced Pluripotent Stem Cells (hiPSC)-Derived Pancreatic Progenitors

            Luiza Ghila, Thomas Aga Legøy, and Simona Chera

24. Highly Efficient Differentiation of Human Pluripotent Stem Cells into Pancreatic Progenitors Co-Expressing PDX1 and NKX6.1

            Bushra Memon and Essam M. Abdelalim

25. Derivation of Three Dimensional Human Induced Pluripotent Stem Cell Derived Vocal Fold Mucosa for Clinical and Pharmacological Applications

            Lungova Vlasta and Susan L. Thibeault

26. Generation of Human Induced Pluripotent Stem Cells Using Endothelial Progenitor Cells Derived from Umbilical Cord Blood and Adult Peripheral Blood

27. Development of a Blood Brain Barrier Permeability Assay Using Human Induced Pluripotent Stem Cell Derived Brain Endothelial Cells

            Claudie Charlebois, Jez Huang, Caroline Sodja, Maria Ribecco-Lutkiewicz, Ewa Baumann, Danica B. Stanimirovic, and Anna Jezierski

28. Revised “hPSC-Sac Method” for Simple and Efficient Differentiation of Human Pluripotent Stem Cells to Hematopoietic Progenitor Cells

            Akinori Yuzuriha and Koji Eto

29. Efficient Generation of iPSC-Derived Hematoendothelial Progenitors and Specification toward T-Cell Lineage

            Siriwal Suwanpitak, Nutchanawan Promnakhon, Ratchapong Netsrithong, and Methichit Wattanapanitch

30. Derivation and Characterization of Mesenchymal Stem Cells from iPS Cells

            Qingguo Zhao and Fei Liu

31. Human Pluripotent Stem Cell Differentiation to Microglia

            Laraib Ijaz, Madhura Nijsure, and Valentina Fossati

32. Human Induced Pluripotent Stem Cell-Derived Microglia (hiPSC-Microglia)

            Amanda McQuade and Mathew Blurton-Jones

33. Isolation and Culture of Human Induced Pluripotent Stem Cell-Derived Cerebral Organoid Cells

34. A Simple Method for Generating, Clearing, and Imaging Pre-Vascularized 3D Adipospheres Derived from Human iPS Cells

            Xi Yao and Christian Dani

35. A Method for In Vitro Fabrication of Hybrid Bone/Cartilage Tissue Using Mouse Induced Pluripotent Stem Cells

            Phoonsuk Limraksasin and Hiroshi Egusa

36. Differentiation of Human Induced Pluripotent Stem Cells (hiPSC) into Endothelial- Type Cells and Establishment of an In Vitro Blood-Brain Barrier Model

            Yuan Li, Georg C. Terstappen, and Wandong Zhang

37. CRISPR/Cas9-Mediated Introduction of Specific Heterozygous Mutations in Human Induced Pluripotent Stem Cells

            Karina O. Brandão, Catarina Grandela, Loukia Yiangou, Christine L. Mummery, and Richard P. Davis

38. CRISPR/Cas9-Mediated Gene Knock-Out and Knock-In Human iPSCs

            Paolo Petazzi, Pablo Menéndez, and Ana Sevilla

39. Generation of Monoclonal iPSC Lines with Stable Cas9 Expression and High Cas9 Activity

40. CRISPR/Cas9-Mediated Genome Editing to Generate Clonal iPSC Lines

            Carla Sanjurjo-Soriano, Nejla Erkilic, Daria Mamaeva, and Vasiliki Kalatzis

41. CRISPR/Cas9 Ribonucleoprotein Complex-Mediated Efficient B2M Knockout in Human Induced Pluripotent Stem Cells (iPSCs)

            Nontaphat Thongsin and Methichit Wattanapanitch

42. Embryonal Carcinoma and Glioblastoma Cell Lines Derived from Monkey Induced Pluripotent Stem Cells

43. Methods for Isolation and Reprogramming of Various Somatic Cell Sources into iPSCs

            Shunit Neeman-Egozi, Polina Baskin, and Ofer Binah

44. The Characteristics of Human iPS Cells and siRNA Transfection under Hypoxia

            Kouji Sugimoto and Yoshihiko Hayashi

45. mRNA-Based Reprogramming under Xeno-Free and Feeder-Free Conditions

            Jakob Jeriha, Nikola Kolundzic, Preeti Khurana, Andrea Perez-Dominguez, and Dusko Ilic

46. Non-Modified RNA-Based Reprogramming of Human Dermal Fibroblasts into Induced Pluripotent Stem Cells

            Immacolata Belviso, Franca Di Meglio, Veronica Romano, Stefania Montagnani, and Clotilde Castaldo

47. Efficient Induction of Primate iPS Cells Using a Combination of RNA Transfection and Chemical Compounds

            Toshiaki Watanabe and Erika Sasaki

48. Generation and Cultivation of Transgene-Free Macaque and Baboon iPSCs under Chemically-Defined Conditions

49. Generation of Marmoset Monkey iPSCs with Self-Replicating VEE-mRNAs in Feeder-Free Conditions

            Stoyan G. Petkov and Rüdiger Behr

50. Differentiation of Human Induced Pluripotent Stem Cells into Definitive Endoderm Using Simple Dialysis Culture Device

            Hyun Jin Choi, Fuad Gandhi Torizal, Marie Shinohara, and Yasuyuki Sakai

51. Detecting and Modulating ER Stress to Improve Generation of Induced Pluripotent Stem Cells

52. Gene Editing in Human Induced Pluripotent Stem Cells Using Doxycycline-Inducible CRISPR-Cas9 System

            Vasanth Thamodaran, Sonam Rani, and Shaji R. Velayudhan

53. Derivation of Clinical-Grade Induced Pluripotent Stem Cell Lines from Erythroid Progenitor Cells in Xenofree Conditions

            Gurbind Singh, Kannan V. Manian, Chitra Premkumar, Alok Srivastava, Dolly Daniel, and Shaji R. Velayudhan

54. Generation of Murine Induced Pluripotent Stem Cells through Transposon-Mediated Reprogramming

            Thirumala R. Talluri, Dharmendra Kumar, and Wilfried A. Kues

55. Human Pluripotent Stem Cells for High-Throughput Drug Screening and Characterization of Small Molecules

            Seungmi Ryu, Pei-Hsuan Chu, Claire Malley, John Braisted, Pinar Ormanoglu, Ruili Huang, Misha Itkin, Zina Itkin, Paul Shinn, Carleen Klumpp-Thomas, Sam Michael, Carlos A. Tristan, Anton Simeonov, and Ilyas Singeç

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts