Somatic Stem Cells, Softcover reprint of the original 1st ed. 2012 Methods and Protocols Methods in Molecular Biology Series, Vol. 879

 Stem cells are found in almost all organisms from the early stages of development to the end of life. There are several types of stem cells and all of them may prove useful for medical research; however, each of the different types has both promise and limitations. Somatic Stem Cells: Methods and Protocols presents selected genetic, molecular, and cellular techniques used in somatic stem cell research and its clinical application. Chapters focus on the isolation, characterization, purity, plasticity, and clinical uses of somatic stem cells from a variety of human and animal tissues. Written in the highly successful Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Through and intuitive, Somatic Stem Cells: Methods and Protocols seeks to provides scientists with the fundamental techniques of stem cell research and its potential application in regenerative medicine.

Section I: Introduction

1. Current Thoughts on the Therapeutic Potential of Stem Cell

Pranela Rameshwar

Section II: Stem Cell Study in Lower Organism

2. A Unique FACS Method to Isolate Stem Cells in Planarian

Tetsutaro Hayashi and Kiyokazu Agata

 3. Identification of Neural Stem Cells in the Drosophila Larval Brain

Mo Weng, Hideyuki Komori, and Cheng-Yu Lee

4. Generation and Staining of Intestinal Stem Cell Lineage in Adult Midgut

Shree Ram Singh, Manoj K. Mishra, Steven X. Hou, and Maduri Kango-Singh 

Section III: Stem Cell Study in Murine and Human Model

5. Developing a Quantitative In Vivo Tissue Reconstitution Assay to Assess the Relative Potency of Candidate Populations of Mouse Oesophageal Epithelial Cells

Daniel Croagh, Rick Redvers, Wayne A. Phillips, and Pritinder Kaur

6. Identification, Isolation, and Culture of Intestinal Epithelial Stem Cells from Murine Intestine

Gracz, A.D., Puthoff, B.J., Magness, S.T.

 7. Isolation and Characterization of Distal Lung Progenitor Cells

Barbara Driscoll, Alex Kikuchi, Allison N. Lau, Jooeun Lee, Raghava Reddy, Edwin Jesudason, Carla F. Kim,
David Warburton

 8. Transplantation of Mouse Fetal Liver Cells for Analyzing the Function of Hematopoietic Stem and Progenitor Cells

Kristbjorn Orri Gudmundsson, Steven W. Stull, and Jonathan R. Keller

9. Convenient and Efficient Enrichment of the CD133+ Liver Cells From Rat Fetal Liver as a Source of Liver Stem/Progenitor Cells

Weihui Liu, Nan You,  Kefeng Dou

 10. Assessing the Potential Clinical Utility of Transplantations of Neural and Mesenchymal Stem Cells for Treating Neurodegenerative Diseases    

Laurent Lescaudron, C.Boyer, Virginie Bonnamain, KD. Fink, X. Lévêque, J. Rossignol, V. Nerrière-Daguin, AC Malouet, F. Lelan, N.D Dey, D. Michel-Monigadon, M. Lu, I. Neveu, S. von Hörsten, P. Naveilhan and GL Dunbar

 11. Functional Identification of Neural Stem Cell-Derived Oligodendrocytes

Sofia Grade,Fabienne Agasse, Liliana Bernardino, João O. Malva

12. Stem/Progenitor Cells in Murine Mammary Gland: Isolation and Functional Characterization

Abhik Bandyopadhyay, Qiaoxiang Dong and Lu-Zhe Sun

 13. A Reporter Assay to Detect Transfer and Targeting of miRNAs in Stem Cell-Breast Cancer Co-Cultures

Steven J. Greco, Shyam A. Patel, Pranela Rameshwar

 ^{14. Isolation, Culture and Osteogenic/chondrogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cells}

^{Susanne Grässel, Sabine Stöckl, Zsuzsa Jenei-Lanzl}

 ^{15. Obtaining Freshly Isolated and Cultured Mesenchymal Stem Cells from Human Adipose Tissue}

^{Andrew C. Boquest and Philippe Collas}

 ^{16. Collection, Processing and Banking of Umbilical Cord Blood Stem Cells for Transplantation and Regenerative Medicine}

^{Michael S. Badowski and David T. Harris}

17. Generation of Functional Islets from Human Umbilical Cord and Placenta Derived Mesenchymal Stem Cells

Sachin Kadam, Vijayendran Govindasamy, and Ramesh Bhonde

 18. Isolation and Characterization of Human Prostate Stem/Progenitor Cells

Changyong Guo, Baohui Zhang, and Isla P. Garraway

 19. Isolation and Expansion of Adult  Cardiac Stem/Progenitor Cells in the Form of Cardiospheres from Human Cardiac Biopsies and Murine Hearts

 Isotta Chimenti, Roberto Gaetani, Lucio Barile, Elvira Forte, Vittoria Ionta, Francesco Angelini, Giacomo Frati, Elisa Messina, Alessandro Giacomello.

 20. Isolation and Differentiation of Human Cardiomyocyte Progenitor Cells Into Cardiomyocytes

Anke M. Smits, Angelique A. van Oorschot and Marie-José Goumans

 21. Isolation, Expansion and Characterization of Human Islet-Derived Progenitor Cells

Mugdha V. Joglekarand Anandwardhan A. Hardikar

 22.  Isolation and Characterization of Resident Mesenchymal Stem Cells in Human Glomeruli

Stefania Bruno and Giovanni Camussi

 23. Endothelial Colony Forming Progenitor Cell Isolation and Expansion

Nicole A. Hofmann, Andreas Reinisch and Dirk Strunk

 24. Isolation and Characterization of Stem Cell-Enriched Human and Canine Hair Follicle Keratinocytes

Manabu Ohyama and Tetsuro Kobayashi

 25. Human Salivary Gland Stem Cells: Isolation, Propagation and Characterization

Silke Schwarz and Nicole Rotter

 26. Identification, Isolation, Characterization and Banking of Human Dental Pulp Stem Cells

Virginia Tirino, Francesca Paino and Gianpaolo Papaccio

 27. Isolation and Differentiation Potential of Fibroblast-Like Stromal Cells Derived from Human Skin

Hsing-I Huang and Chung-Zu Wu

28. Immortalization of Human Mesenchymal Stromal Cells with Telomerase and Red Fluorescence Protein Expression

Chao-Ling Yao and Shiaw-Min Hwang

 29. Genetic Modification of Mesenchymal Stem Cells

Andréia Escosteguy Vargas, Melissa Medeiros Markoski, Andrés Delgado Cañedo, Flávia Helena da Silva and Nance Beyer Nardi

30. Methodology, Biology and Clinical Applications of Human Mesenchymal Stem Cells

Melissa Camassola, Luisa Maria Gomes de Macedo Braga, Pedro Cesar Chagastelles and Nance Beyer Nardi

^{31. In Vitro Production of Enucleated Red Blood Cells from Hematopoietic Stem and Progenitor Cells}

^{Kenichi Miharada and Yukio Nakamura}

32. Methods for Cancer Stem Cell Detection and Isolation

Virginia Tirino, Vincenzo Desiderio, Francesca Paino and Gianpaolo Papaccio

33. Biocompatible Nanoparticle Labelling of Stem Cells and their Distribution in Brain

Ashish K. Rehni, Thakur Gurjeet Singh, Mansi Chitkara, and I. S. Sandhu

Presents selected genetic, molecular, and cellular techniques used in somatic stem cell reserach and its clinical application

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras