Somatic Stem Cells (2^nd Ed., Softcover reprint of the original 2nd ed. 2018) Methods and Protocols Methods in Molecular Biology Series, Vol. 1842

This second edition volume expands on the previous edition with updated discussions on new genetic, molecular, and cellular methods used to study somatic stem cells. The chapters in this book focus on the isolation, classification, purity, and plasticity of these stem cells in a variety of organic tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Somatic Stem Cells: Methods and Protocols, Second Edition is a valuable resource for both novice and experienced molecular biologists, developmental biologists, tissue engineers, and geneticists who are interested in stem cell research and its potentials in regenerative medicine.

Preface…
Table of Contents…
Contributing Authors…

Part I Introduction

1. An Update on the Therapeutic Potential of Stem Cell
Pranela Rameshwar, Caitlyn A. Moore, Niloy N. Shah, and Caroline P. Smith 

Part II Stem Cell Study in Model Organisms and Human

2. Single-Step Plasmid Based Reprogramming of Human Dermal Fibroblasts to Induced Neural Stem Cells
Luis Azmitia and Philipp Capetian

3. Isolation and Analysis of Mesenchymal Progenitors of the Adult Hematopoietic Niche
Mayra Garcia, Lihong Weng, Xingbin Hu, and Ching-Cheng Chen

4. Identification and Isolation of Mice and Human Hematopoietic Stem Cells
Bijender Kumar and Srideshikan Sargur Madabushi

5. Identification and Characterization of Hair Follicle Stem Cells
Xiaoyang Wang, Yizhan Xing, and Yuhong Li 

6. Methods of Mesenchymal Stem Cell Homing to the Blood-Brain Barrier
Peter Conaty, Lauren S. Sherman, Yahaira Naaldijk, Henning Ulrich, Alexandra Stolzing, and Pranela Rameshwar

7. 3D Bioprinting and Stem Cells
Caitlyn A. Moore, Niloy N. Shah, Caroline P. Smith, and Pranela Rameshwar

8. Characterization of Gastrospheres Using 3D Coculture System
Carlos Antônio do Nascimento Santos, Radovan Borojevic, Luiz Eurico Nasciutti, and Christina MaedaTakiy

9. Markers and Methods to Study Intestinal Stem Cells
Nathan Pinto, Beyoncé Carrington, Catharine Dietrich, and Shree Ram Singh

10. Quantitative Analysis of Intestinal Stem Cell Dynamics using Microfabricated Cell Culture Arrays
Leigh A. Samsa, Ian A. Williamson, and Scott T. Magness

11. Detection, Labeling, and Culture of Lung Stem and Progenitor Cells
Ivan Bertoncello, Gianni Carraro, and Jonathan L. McQualter

12. Isolation, Characterization, and Differentiation of Mouse Cardiac Progenitor Cells
Santosh Kumar Yadav and Paras Kumar Mishra

13. Isolating and Characterizing Adipose-Derived Stem Cells
Guangpeng Liu and Xi Chen

14. Enzyme-Free Isolation of Adipose-Derived Mesenchymal Stem Cell
Lauren S. Sherman, Alexandra Condé-Green, Vasanth S. Kotamarti, Edward S. Lee, and Pranela Rameshwar

15. Identification and Characterizations of Annulus Fibrosus-Derived Stem Cells
Qianping Guo, Pinghui Zhou, and Bin Li

16. The Maintenance of Tendon Stem/Progenitor Cells in Culture
Jianying Zhang and James H-C. Wang

17. Intravital Imaging to Understand Spatiotemporal Regulation of Osteogenesis and Angiogenesis in Cranial Defect Repair and Regeneration
Xinping Zhang

18. Beating Heart Cells from Hair Cells
Robert M. Hoffman and Yasuyuki Amoh

19. Generation of FLIP and FLIP-FlpE Targeting Vectors for Bi-Allelic Conditional and Reversible Gene Knockouts in Mouse and Human Cells
Bon-Kyoung Koo

20. Analytical Platforms and Techniques to Study Stem Cells Metabolism 
Christine Tang, Kevin Chen, Aleksandar Bajic, William T. Choi, Dodge L. Baluya, and Mirjana Maletic-Savatic

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts