Stem Cell Mobilization, Softcover reprint of the original 1st ed. 2012 Methods and Protocols Methods in Molecular Biology Series, Vol. 904

Stem cell mobilization represents a transient increase in the levels of circulating stem and progenitor cells. In Stem Cell Mobilization: Methods in Protocols, expert researchers in the field detail cell mobilization methodology and  recent developments in the field for basic and biomedical research community. Specifically clinical hematopoietic progenitor cell mobilization protocols, frontiers in mobilization and analysis of non-hematopoietic progenitors, mesenchymal progenitor cells, monocyte-derived fibroblast progenitors, and very small embryonic like cells. Written in the highly successful Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Stem Cell Mobilization: Methods and Protocols seeks to aid scientists in the further study of this important stem cell research.

1. Mobilization of Hematopoietic Stem/progenitor Cells: General Principles and Molecular Mechanisms

Halvard Bonig and Thalia Papayannopoulou

2. Quantifying Hematopoietic Stem and Progenitor Cell Mobilization

Shiri Gur-Cohen, Kfir Lapid, Tsvee Lapidot

3. Hematopoietic Stem Cell Mobilization with G-CSF

Chitra Hosing

4. Hematopoietic Stem Cell Mobilization with Agents Other Than G-CSF

Jonathan Hoggatt and Louis M. Pelus

5. Hematopoietic Stem Cell Mobilization: A Clinical Protocol

Gina Pesek and Michele Cottler-Fox

6. Monitoring Blood for CD34+ cells to Determine Timing of Hematopietic Progenitor Cells (HPC) Apheresis

M.  Louette Vaughn and Edmund K. Waller

7. Hematopoietic Progenitor Cell Collection

S. Darlene Marlow and Myra House

8. Managing Apheresis Complications during the Hematopoietic Stem Cell Collection

S. Darlen Marlow and Myra House

9. Hematopoietic Progenitor Cell Apheresis Processing

Eleanor S. Hamilton and Edmund K. Waller

10. Toxicities of Mobilized Stem Cell Infusion

11. Mobilization of Hematopoietic Stem Cells by Depleting Bone Marrow Macrophages

Valérie Barbier Ingrid G. Winkler and Jean-Pierre Lévesque

12. Combinatorial Stem Cell Mobilization in Animal Models

Simon C Pitchford and Sara M Rankin

13. Vascular Progenitor Cell Mobilization

14. Evaluation of Circulating Endothelial Precursor Cells in Cancer Patients

Francesco Bertolini, Patrizia Mancuso, Liat Benayoun, Svetlana Gingis-Velitski, and Yuval Shaked

15. Tracking Inflammation-Induced Mobilization of Mesenchymal Stem Cells

Erika L. Spaeth, Shannon Kidd and Frank C Marini

16. Differentiation of Circulating Monocytes into Fibroblast-like Cells

Darrell Pilling and Richard H. Gomer

17. Enumeration of Very Small Embryonic Like Stem Cells in Peripheral Blood

Rui Liu and Mariusz Z. Ratajczak

18. Generation of Vascular Niche for Studying Stem Cell Homeostasis

Jason M. Butler and Shahin Rafii

19. Studying Vascular Progenitor Cells in a Neonatal Mouse Model

Kirsten A. Kienstra and Karen K. Hirschi

20. Progenitor Cell Mobilization from Extramaedullary Organs

Mikhail G. Kolonin

Overviews the current standing in cell mobilization methodology and to outline recent developments

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts