Handbook of Vascular Biology Techniques, 2015
Coordonnateurs : Slevin Mark, McDowell Garry
A wide range of research methods for the study of vascular development, from basic laboratory protocols to advanced technologies used in clinical practice, are covered in this work. A range of methodologies such as molecular imaging platforms and signalling analysis, along with tumour models are collated here.
Four sections explore in vitro techniques, in vivo and ex vivo manipulations, imaging and histological analysis and other novel techniques in vascular biology. Readers will discover basic methodologies used for analysis of endothelial cell growth in vitro, including co-culture models of vessel formation. Authors also explore isolation and purification of cells and methods for analysis of data and visualization of localized vasculature with modern imaging platforms. Both animal models and human disease are covered in this work.
Each chapter contains helpful sections on trouble shooting, additional notes and links, supporting the reader to carry out protocols. This book will appeal to students, researchers and medical professionals working in all vascular-linked fields such as cardio- and cerebrovascular, cancer and dementia.
Preface
Section 1 In vitro techniques
Chapter 1: In vitro angiogenesis assay - endothelial migration, proliferation, and tube formation
Chapter 2: Endothelial cell tube formation on basement membrane to study cancer neoangiogenesis
Chapter 3: Induction of hypoxia in vascular endothelial cell culture
Chapter 4: Evaluating In Vitro Angiogenesis using Live Cell Imaging
Chapter 5: Isolation of Endothelial Progenitor Cells (EPCs)
Chapter 6: Culture and Maintenance of Human Embryonic Stem Cells: A Potential Source for Vasculargenesis
Chapter 7: Assessment of vascular function and contractility, ex vivo.
Chapter 8: Measurement of Aβ uptake by cerebrovascular smooth muscle cells
Chapter 9: Measurement of Intracellular Ca2+ in Human Endothelial cells
Section 2: In vivo and ex vivo manipulations
Chapter 10: Evaluation of angiogenesis and arteriogenesis in a mouse model of prolonged cerebral hypoperfusion
Chapter 11: Sponge implant model of inflammatory angiogenesis
Chapter 12: The chick embryo chorioallantoic membrane assay
Chapter 13: Dorsal air sac assay
Chapter 14: Scanning Electron Microscopy of Blood Vascular Corrosion Casts (SEM on VCC) in mammals
Chapter 15: Hypoxia-induced retinal angiogenesis in adult zebrafish
Chapter 16: Angiogenesis in regenerating adult zebrafish tail fin
Chapter 17: Methods for studying developmental angiogenesis in zebrafish
Chapter 18: Isolation and Expansion of Brain Microvascular Endothelial Cells
Section 3: Imaging and histological analysis
Chapter 19: Adipose angiogenesis
Chapter 20: Assessing tumor angiogenesis in histological samples
Chapter 21: Whole-mount immunostaining methods to study the blood and lymphatic vasculature in the embryonic mouse skin and adult mouse cornea.
Chapter 22: Computed Tomography Angiography: Fundamental techniques and data interpretation
Chapter 23: Magnetic Resonance Angiography: Fundamental Techniques and Data Interpretation
Chapter 24: Single-Photon Emission Tomography of the brain in vascular pathology
Section 4: Miscellaneous novel techniques in vascular biology
Chapter 25: Enhancing Endothelialisation of Artificial/Engineered Blood Vessels Using Structural Cues
Chapter 26: Preparation of liposomes with dual fluorophores to follow real-time content release in vivo
Chapter 27: Vascular Flow Modelling using Computational Fluid Dynamics
Chapter 28: Reverse Transcription Real-Time PCR Protocol for gene expression analyses
Chapter 29: Oscillations, Feedback and Bifurcations in Mathematical Models of Angiogenesis and Haematopoiesis
Chapter 30: Genomic microarray analysis
Chapter 31: Selection of appropriate housekeeping genes for quality control
Chapter 32: Endothelial transcriptomic analysis
Chapter 33: Protocol for Multiplex Amplicon Sequencing using Barcoded Primers
Chapter 34: Flow cytometry Enumeration of Hematopoietic & Progenitor Stem Cells: Identification and Quantification
Chapter 35: A Scheme for the Development and Validation of Enzyme Linked Immunosorbent Assays (ELISA) for Measurement of Angiogenic Biomarkers in Human Blood
Chapter 36: Analysis of Phosphorylated Protein Kinases in Endothelial cells by Flow Cytometry
Enablers researchers to employ all research techniques in vascular biology
Guides the reader step-by-step, through current and experimental protocols
Includes numerous explanatory and supportive diagrams
Includes supplementary material: sn.pub/extras
Date de parution : 10-2016
Ouvrage de 477 p.
15.5x23.5 cm
Date de parution : 04-2015
Ouvrage de 477 p.
15.5x23.5 cm
Thèmes de Handbook of Vascular Biology Techniques :
Mots-clés :
Angiogenesis; Experimental; Methodology; Protocols; Vascular
