Vascular Morphogenesis, Softcover reprint of the original 1st ed. 2015 Methods and Protocols Methods in Molecular Biology Series, Vol. 1214

Vascular Morphogenesis: Methods and Protocols provides a range of techniques for studying vascular morphogenesis in vivo and in vitro, reflecting advances in the field. Endothelial cell signaling is currently believed to promote fundamental cues for cell fate specification, embryo patterning, organ differentiation and postnatal tissue remodeling. Understanding the concept of vascular bed specificity represents a major challenge for future investigations. Indeed, one of the most interesting theoretical perspectives and practical applications of endothelial cell signaling is the possibility for these cells to maintain their inductive potential during adult life. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, Vascular Morphogenesis: Methods and Protocols will serve as a instructive and useful reference for cell biologists, anatomists, pathologists and physiologists, as well as all those interested in this area of biological study.

1. The Development of the Vascular System: An Historical Overview

            Domenico Ribatti, Beatrice Nico, and Enrico Crivellato

2. The Corneal Pocket Assay

            Marina Ziche and Lucia Morbidelli

3. The Mouse Hindbrain: An In Vivo Model to Analyze Developmental Angiogenesis

            Alice Plein, Christiana Ruhrberg, and Alessandro Fantin

4. Three Dimensional In Vitro Assay of Endothelial Cell Invasion and Capillary Tube Morphogenesis

            Laura di Blasio, Federico Bussolino, and Luca Primo

5. Vascular Casting for the Study of Vascular Morphogenesis

            Maximilian Ackermann and Moritz A. Konerding

6. Cell-Based Computational Modeling of Vascular Morphogenesis Using Tissue Simulation Toolkit

            Josephine T. Daub and Roeland Merks

7. miRNAs Expression Profile in Zebrafish Developing Vessels

            Emma Ristori and Stefania Nicoli

8. Imaging of Cardiovascular Development in Mammalian Embryos Using Optical Coherence Tomography

            Monica D. Garcia, Andrew L. Lopez III, Kirill V. Larin, and Irina V. Larina

9. Live Confocal Microscopy of the Developing Mouse Embryonic Yolk Sac Vasculature

            Andrew L. Lopez III, Monica D. Garcia, Mary E. Dickinson, and Irina V. Larina

10. Zebrafish Embryo Intersegmental Vessels: A Tool for Investigating Sprouting Angiogenesis

            Chiara Tobia, Giuseppina Gariano, Jessica Guerra, and Marco Presta

11. Avian Area Vasculosa and CAM as Rapid In Vivo Pro- and Anti-Angiogenic Models

            Andrew N. Makanya, Beata Styp-Rekowska, Ivanka Dimova,  and Valentin Djonov

12. Investigating In Vitro Angiogenesis by Computer-Assisted Image Analysis and Computational Simulation

            Diego Guidolin, Caterina Fede, Giovanna Albertin, and Raffaele De Caro

13. A Chimeric Embryoid Body Model to Study Vascular Morphogenesis

            Yanmei Qi, Siavash Saadat, Jie Liu, Alan M. Graham, and Shaohua Li

14. Avians as a Model System of Vascular Development

            Michael Bressan and Takashi Mikawa

15. Microfluidic Model of Angiogenic Sprouting

            Jonathan W. Song, Despina Bazou, and Lance L. Munn

16. The Rat Aortic Ring Model of Angiogenesis

            Alfred C. Aplin and Roberto F. Nicosia

17. The Co-Culture Organotypic Assay of Angiogenesis

            Jennifer L. Allen and Harry Mellor

Includes cutting-edge methods and protocols in vascular morphogenesis research

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras