Vascular Tissue Engineering, 1st ed. 2022 Methods and Protocols Methods in Molecular Biology Series, Vol. 2375

This volume explores the latest techniques used to study the field of tissue engineered vascular grafts (TEVGs). The chapters in this book cover a wide array of topics such as deriving vascular cells from monocytes and induced pluripotent stem cells; engineering vascular grafts using various biomaterials and stem cells, stem cell-derived, or primary vascular cells; biomaterial modification by anticoagulation molecules; vascular bioengineering technologies such as 3D bioprinting; and fabrication of TEVGs with different geometry and multiphase structures. This book also features protocols for grafting and evaluation of vascular grafts in animal models, vascular imaging in animals, and the quantification of blood vessel permeability. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and practical, Vascular Tissue Engineering: Methods and Protocols is a valuable resource for biomedical engineers, cell biologists, vascular surgeons, doctors, and nurses. 

Preface…
Table of Contents…
Contributing Authors…

1. Differentiating Human Pluripotent Stem Cells to Vascular Endothelial Cells for Regenerative Medicine, Tissue Engineering, and Disease Modeling
Taylor Bertucci, Shravani Kakarla, Diana Kim, and Guohao Dai

2. Generating Monocyte Derived Endothelial-Like Cells for Vascular Regeneration
Randall J. Smith Jr. and Stelios T. Andreadis

3. Methods for Differentiating hiPSCs into Vascular Smooth Muscle Cells
Mei-Lan Li, Jiesi Luo, Matthew W. Ellis, Muhammad Riaz, Yasmeen Ajaj, and Yibing Qyang

4. On-Site Differentiation of Human Mesenchymal Stem Cells into Vascular Cells on Extracellular Matrix Scaffold under Mechanical Stimulations for Vascular Tissue Engineering
Na Li, Alex P. Rickel, and Zhongkui Hong

5. End-Point Immobilization of Heparin on Electrospun Polycarbonate-Urethane Vascular Graft
Jun Fang and Song Li

6. Microfluidic Co-Axial Bioprinting of Hollow, Standalone, and Perfusable Vascular Conduits
Sushila Maharjan, Jacqueline Jialu He, Li Lv, Di Wang, and Yu Shrike Zhang

7. In Situ Fabrication and Perfusion of Tissue Engineered Blood Vessel Microphysiological System
Xu Zhang, Nadia O. Abutaleb, Ellen Salmon, and George A. Truskey

8. Peritoneal Pre-Conditioning Method for In Vivo Vascular Graft Maturation Utilizing a Porous Pouch
Mahyar Sameti and Christopher A. Bashur

9. Fabrication of a Completely Biological and Anisotropic Human Mesenchymal Stem Cell-Based Vascular Graft
Weilue He, Dhavan Sharma, Wenkai Jia, and Feng Zhao

10. Engineering Vascular Grafts with Multiphase Structures
Bryan D. James and Josephine B. Allen

11. Fabrication of Small Diameter Tubular Grafts for Vascular Tissue Engineering Applications using Mulberry and Non-Mulberry Silk Proteins
Prerak Gupta and Biman B. Mandal

12. Fabrication and Evaluation of Tissue-Engineered Vascular Grafts with Hybrid Fibrous Structure
He Wang and Qiang Zhao

13. Controlling Pore Size of Electrospun Vascular Grafts by Elecrospraying of Poly(Ethylene Oxide) Microparticles
Muhammad Rafique, Adam C. Midgley, Tingting Wei, Lianyong Wang, Deling Kong, and Kai Wang

14. Injectable Hydrogels for Vascular Tissue Engineering 
Fengqiao Li, William Ho, and Xiaoyang Xu

15. Rabbit Surgery Protocol for End-to-End and End-to-Side Vascular Graft Anastomosis
Yuan Yao, YeJin Jeong, Aung Moe Zaw, Marek Kukumberg, and Evelyn K.F. Yim

16. Vascular Imaging in Small Animals Using Clinical Ultrasound Scanners
Aung Moe Zaw, Richard Shannuan, Yuan Yao, YeJin Jeong, Billy Y.S. Yiu, Adrian J. Y. Chee, Alfred C. H. Yu, and Evelyn K.F. Yim

17. Vascular Graft Implantation using a Bilateral End-to-Side Aortoiliac Preclinical Model
Khanh P. Nguyen, Monica T. Hinds, and Deirdre E.J. Anderson

18. Quantification of In Vitro Blood-Brain Barrier Permeability
Bingmei M. Fu

19. Subcellular Force Quantification of Endothelial Cells using Silicone Pillar-Arrays
Mohanish K. Chandurkar and Sangyoon J. Han

20. Computational Assessment of Hemodynamics Vortices within the Cerebral Vasculature using Information Entropy
Kevin Sunderland, Feng Zhao, and Jingfeng Jiang

Subject Index List…

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts