Plant Membrane Proteomics, 1st ed. 2018 Methods and Protocols Methods in Molecular Biology Series, Vol. 1696

This volume provides comprehensive and detailed  protocols that discuss proteomic techniques, plant endosomes, and isolation of organelles and subcellular fractions. The chapters in this book explore numerous plant species and cover topics, such as isolation and purity assessment of membranes from Norway spruce; proteomic analysis of nuclei; analyzing the vacuolar membrane (tonoplast) proteome; isoforms of a thylakoid-bound protein; assay of plasma membrane H+-ATPase in plant tissue under abiotic stresses; and identification and characterization of plant membrane proteins using ARAMEMNON. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and thorough, Plant Membrane Proteomics: Methods and Protocols is a valuable resource that promotes the use of plant membrane proteomics to develop the future of the field.

Table of Contents…

Contributing Authors…

1. Free Flow Zonal Electrophoresis for Fractionation of Plant Membrane Compartments prior to Proteomic Analysis

Bronwyn J. Barkla

2. Isolation and Purity Assessment of Membranes from Norway Spruce

Enni Väisänen, Junko Takahashi, Luis A. Jiménez Barboza, Xianbao Deng, Teemu H. Teeri, Kurt V. Fagerstedt, Sabine Lüthje, and Anna Kärkönen

3. Nuclear Proteome: Isolation of Intact Nuclei, Extraction of Nuclear Proteins and 2-DE Analysis

Aarti Pandey, Subhra Chakraborty, and Niranjan Chakraborty

4. Identification of Plant Nuclear Proteins Based on a Combination of Flow Sorting, SDS-PAGE, and LC-MS/MS Analysis

Ivo Chamrád, Jana Uřinovská, Beáta Petrovská, Hana Jeřábková, René Lenobel, Jan Vrána, Jaroslav Doležel, and Marek Šebela

5. Isolation, Purity Assessment, and Proteomic Analysis of Nuclei

Setsuko Komatsu

6. Proteomic Analysis of Rice Golgi Membranes Isolated by Floating through Discontinuous Sucrose Density Gradient

Kazusato Oikawa, Takuya Inomata, Yoshitoshi Hirao, Tadashi Yamamoto, Marouane Baslam, Kentaro Kaneko, and Toshiaki Mitsui

7. Analyzing the Vacuolar Membrane (Tonoplast) Proteome

Miwa Ohnishi, Katsuhisa Yoshida, and Tetsuro Mimura

8. Preparation of Membrane Fractions (Envelope, Thylakoids, Grana and Stroma Lamellae) from Arabidopsis Chloroplasts for Quantitative Proteomic Investigations and Other Studies

Lucas Moyet, Daniel Salvi, Martino Tomizioli, Daphné Seigneurin-Berny, and Norbert Rolland

9. Isolation of Intact Thylakoid Membranes from Heterocytes of Filamentous, Nitrogen-Fixing Cyanobacteria

Ann Magnuson and Tanai Cardona

10. Targeted Quantification of Isoforms of a Thylakoid-Bound Protein: MRM Method Development

Roque Bru-Martínez, Ascensión Martínez-Márquez, Jaime Morante-Carriel, Susana Sellés-Marchart, María José Martínez-Esteso, José Luis Pineda-Lucas, and Ignacio Luque

11. Sample Preparation for Analysis of the Plant Mitochondrial membrane Proteome

Christine Schikowsky, Beate Thal, Hans-Peter Braun, and Holger Eubel

12. Plasma Membrane Proteomics of Arabidopsis Suspension Cultured Cells Associated with Growth Phase using Nano-LC-MS/MS

Bin Li, Daisuke Takahashi, Yukio Kawamura, and Matsuo Uemura

13. Mini-Scale Isolation and Preparation of Plasma Membrane Proteins from Potato Roots for LC/MS Analysis

Anna M. Jozefowicz, Andrea Matros, Katja Witzel, and Hans-Peter Mock

14. Assay of Plasma Membrane H+-ATPase in Plant Tissues under Abiotic Stresses

Małgorzata Janicka, Anna Wdowikowska, and Grażyna Kłobus

15. Absolute Quantification of In Vitro Expressed Plant Membrane Proteins by Targeted Proteomics (MRM) for the Determination of Kinetic Parameters

Carsten Rautengarten, Berit Ebert and Joshua L. Heazlewood

16. MSE for Label-Free Absolute Protein Quantification in Complex Proteomes

Stefan Helm and Sacha Baginsky

17. Identification and Characterization of Plant Membrane Proteins using ARAMEMNON

Rainer Schwacke and Ulf-Ingo Flügge

18. VANTED: A Tool for Integrative Visualization and Analysis of –Omics Data

Anja Hartmann and Anna Maria Jozefowicz