Plant Protein Secretion, Softcover reprint of the original 1st ed. 2017 Methods and Protocols Methods in Molecular Biology Series, Vol. 1662

This volume aims to provide an update on recent developments in protein secretion studies in plants versus yeast and mammalian systems. This book also discusses case studies that analyze the use of plant protein secretion using various tools and systems. The chapters in this book explore topics such as the study of Golgi-mediated protein traffic in plant cells; actin-based intracellular trafficking in pollen tubes; secretion system for identification of cargo proteins of vacuolar sorting receptors; isolation of the plant exocyst complex; and plant autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Plant Protein Secretion: Methods and Protocols is a valuable resource for researchers interested in furthering their studies in plant protein secretion."

Preface…
Table of Contents…
Contributing Authors…

1.      An Overview of Protein Secretion in Yeast and Animal Cells
Yusong Guo, Feng Yang, and Xiao Tang

2.      An Overview of Protein Secretion in Plant Cells
Kin Pan Chung and Yonglun Zeng

4.      Proteomics Analysis of Secreted Proteins from Cell Microenvironment
Subash Adhikari, Lan Chen, Peiwu Huang, and Ruijun Tian

5.      Using Homology Modelling to Understand the Structural Basis of Specific Interaction of a Plant-Specific AtSar1a/AtSec23a Pair Involved in Protein ER Export
Yap-Shing Nim, Shuangli Sun, and Kam-Bo Wong

6.      Analysis of Golgi-Mediated Protein Traffic in Plant Cells
Wenjin Shen, Zhidan Xiao, Jinbo Shen, and Caiji Gao

7.      Analysis of Membrane Protein Topology in the Plant Secretory Pathway
Jinya Guo, Yansong Miao, and Yi Cali

8.      Semi-Automatic Segmentation of Plant Golgi Stacks in Electron Tomograms Using 3dmod
Keith Ka Ki Mai and Byung-Ho Kang

10.  Transient Expression of Chimeric Fluorescent Reporter Proteins in Pollen Tubes to Study Protein Polar Secretion and Dynamics
Guitao Zhong, Ronghe Liu, Menglong Zhuang, and Hao Wang

11.  Analysis of Actin-Based Intracellular Trafficking in Pollen Tubes
Yuxiang Jiang, Meng Zhang, and Shanjin Huang

12.  Analysis of Phragmoplast Kinetics during Plant Cytokinesis
Pantelis Livanos, Mayank Chugh, and Sabine Müller

13.  Immunofluorescence Analysis of Membrane-Associated Proteins for Clathrin-Mediated Endocytosis in Plant Root Cells
Chao Wang, Xu Yan, Tingting Meng, Tianwei Hu, and Jianwei Pan

14.  In Vivo Interaction Studies by Measuring Förster Resonance Energy Transfer through Fluorescence Lifetime Imaging Microscopy (FRET/FLIM)
Florian Fäßler and Peter Pimpl

15.  Analysis of Nanobody-Epitope Interactions in Living Cells via Quantitative Protein Transport Assays 
Simone Früholz and Peter Pimpl

16.  A Secretion System for Identification of Cargo Proteins of Vacuolar Sorting Receptors 
Jinbo Shen 

17.  Identifying Novel Regulators of Vacuolar Trafficking by Combining Fluorescence Imaging-Based Forward Genetic Screening and In Vitro Pollen Germination
Qiang-Nan Feng and Yan Zhang

19.  Transient Secretory Enzyme Expression in Leaf Protoplasts to Characterize SNARE Functional Classes in Conventional and Unconventional Secretion
Di Sansebastiano Gian Pietro and Barozzi Fabrizio

21.  Analysis of Exocyst-Positive Organelle (EXPO)-Mediated Unconventional Protein Secretion in Plant Cells
Yu Ding and Juan Wang

22.  Isolation of the Plant Exocyst Complex
King Pong Leung and Wilson Chun Yu Lau

23.  Using Microscopy Tools to Visualize Autophagosomal Structures in Plant Cells
Weili Lin and Xiaohong Zhuang

24.  Analysis of Plant Autophagy
Liang Chen, Faqiang Li, and Shi Xiao

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras