Isolation of Plant Organelles and Structures, 1st ed. 2017 Methods and Protocols Methods in Molecular Biology Series, Vol. 1511

1. The Isolation of Plant Organelles and Structures in the Post-Genomics Era

            A. Harvey Millar and Nicolas L. Taylor

2. Approaches to Characterize Organelle, Compartment, or Structure Purity

            Stefanie J. Mueller, Sebastian N.W. Hoernstein, and Ralf Reski

Part I: Isolation of Organelles and Organelle Compartments

3. Isolation of Nuclei and Nucleoli

            Alison F. Pendle and Peter J. Shaw

4. Isolation and Suborganellar Fractionation of Arabidopsis Chloroplasts

            Úrsula Flores-Pérez and Paul Jarvis

5. Isolation of Chromoplasts and Sub-Organellar Compartments from Tomato and Bell Pepper Fruit

6. Leucoplast Isolation and Subfractionation

            William C. Plaxton

7. Isolation of Mitochondria, Their Sub-Organellar Compartments, and Membranes

            Owen Duncan, A. Harvey Millar, and Nicolas L. Taylor

8. Isolation of Arabidopsis Leaf Peroxisomes and the Peroxisomal Membrane

            Sigrun Reumann and Piotr Lisik

9. Isolation of Vacuoles and the Tonoplast

            Jan Zouhar

10. Isolation of Endoplasmic Reticulum and Its Membrane

            G. Eric Schaller

11. Enrichment of Golgi Membranes from Triticum aestivum (Wheat) Seedlings

            Wei Zeng, Berit Ebert, Harriet T. Parsons, Carsten Rautengarten, Antony Bacic, and Joshua L. Heazlewood

12. Isolation of Autolysosomes from Tobacco BY-2 Cells

            Chihiro Takatsuka, Yuko Inoue, and Yuji Moriyasu

13. Isolation of Protein Storage Vacuoles and Their Membranes

            Tomoo Shimada and Ikuko Hara-Nishimura

Part II: Isolation of Other Structures and Compartments

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14. Isolation of the Cell Wall

            Hervé Canut, Cécile Albenne, and Elisabeth Jamet

15. Isolation of Plasmodesmata

            Christine Faulkner and Emmanuelle M.F. Bayer

16. Isolation of Plasma Membrane and Plasma Membrane Microdomains

            Anzu Minami, Daisuke Takahashi, Yukio Kawamura, and Matsuo Uemura

17. Enrichment of the Plant Cytosolic Fraction

18. Isolation of Apoplast

            Erik Andreasson, Kibrom B. Abreha, and Svante Resjö

19. Isolation of Cytosolic Ribosomes

            Hanna Klang Årstrand and Maria V. Turkina

20. Isolation of Plastid Ribosomes

            Kenichi Yamaguchi

21. Isolation of Mitochondrial Ribosomes

            Adam J. Carroll

22. Isolation of Microtubules and Microtubule-Associated Proteins

            Takahiro Hamada and Seiji Sonobe

23. Isolation of Actin and Actin Binding Proteins

24. Purification of 26S Proteasomes and Their Sub-Complexes from Plants

            Richard S. Marshall, David C. Gemperline, and Richard D. Vierstra

Includes cutting-edge methods and protocols for the study of plant organelles and structures

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts