Plant Gravitropism (2^nd Ed., 2nd ed. 2022) Methods and Protocols Methods in Molecular Biology Series, Vol. 2368

1. Plant Gravitropism: From Mechanistic Insights into Plant Function on Earth to Plants Colonizing Other Worlds

            Sabrina Chin and Elison B. Blancaflor

2. Evaluation of Gravitropism in Non-Seed Plants

            Yuzhou Zhang, Lanxin Li, and Jiří Friml

3. Spore Preparation and Protoplast Isolation to Study Gravity Perception and Response in Ceratopteris richardii

4. A Multiplexed, Time-Resolved Assay of Root Gravitropic Bending on Agar Plates

            Takehiko Ogura, Christian Goeschl, and Wolfgang Busch

5. Methods to Quantify Cell Division and Hormone Gradients During Root Tropisms

6. Using the Automated Botanical Contact Device (ABCD) to Deliver Reproducible, Intermittent Touch Stimulation to Plants

            Caleb Fitzgerald, Cullen S. Vens, Nathan Miller, Richard Barker, Matthew Westphall, Johnathan Lombardino, Jerry Miao, Sarah J. Swanson, and Simon Gilroy

7. High-Resolution Kinematic Analysis of Root Gravitropic Bending Using RootPlot

            Aditi Bhat, Cody L. DePew, and Gabriele B. Monshausen

8. Staging of Emerged Lateral Roots in Arabidopsis thaliana

            Sascha Waidmann and Jürgen Kleine-Vehn

9. Methods for a Quantitative Comparison of Gravitropism and Posture Control Over a Wide Range of Herbaceous and Woody Species

            Félix P. Hartmann, Hugo Chauvet-Thiry, Jérôme Franchel, Stéphane Ploquin, Bruno Moulia, Nathalie Leblanc-Fournier, and Mélanie Decourteix

10. The Analysis of Gravitropic Setpoint Angle Control in Plants

            Suruchi Roychoudhry, Marta Del Bianco, and Stefan Kepinski

11. A Flat Embedding Method to Orient Gravistimulated Root Samples for Sectioning

            Utku Avci and Jin Nakashima

12. Conducting Plant Experiments in Space and on the Moon

            Tatsiana Shymanovich and John Z. Kiss

13. Plant Proteomic Data Acquisition and Data Analyses: Lessons from Spaceflight

            Proma Basu, Colin P.S. Kruse, Darron R. Luesse, and Sarah E. Wyatt

14. Blueprints for Constructing Microgravity Analogs

            Karl H. Hasenstein

15. Arabidopsis Growth and Dissection on Polyethersulfone (PES) Membranes for Gravitropic Studies

            Alexander Meyers, Nathan Scinto-Madonich, Sarah Wyatt, and Chris Wolverton

16. Use of Reduced Gravity Simulators for Plant Biological Studies

            Raúl Herranz, Miguel A. Valbuena, Aránzazu Manzano, Khaled Y. Kamal, Alicia Villacampa, Malgorzata Ciska, Jack J.W.A. van Loon, and F. Javier Medina

17. Understanding the Mechanisms of Gravity Resistance in Plants

            Kouichi Soga, Sachiko Yano, Motoshi Kamada, Shouhei Matsumoto, and Takayuki Hoson

18. NASA’s Ground-Based Microgravity Simulation Facility

            Ye Zhang, Jeffery T. Richards, Jessica L. Hellein, Christina M. Johnson, Julia Woodall, Tait Sorenson, Srujana Neelam, Anna Maria J. Ruby, and Howard G. Levine

19. Evaluating the Effects of the Circadian Clock and Time of Day on Plant Gravitropic Responses

            Joseph S. Tolsma, Jacob J. Torres, Jeffrey T. Richards, Imara Y. Perera, and Colleen J. Doherty

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts