Agrobacterium Protocols (3^rd Ed., Softcover reprint of the original 3rd ed. 2015) Volume 2 Methods in Molecular Biology Series, Vol. 1224

Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Third Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together with the 1^st and 2^nd editions, these two volumes offer Agrobacterium-mediated genetic transformation protocols for a total of 76 plant species. For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included.

Volume 2 contains 29 chapters with updated techniques for industrial plants, root plants, nuts and fruits, tropic plants, and other important plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge,Agrobacterium Protocols, Third Edition facilitates the transfer of this rapidly developing technology to all researchers in both fundamental and applied biology.

Part I

Industrial Plants

1

Brassica Rapa

Tom Lawrenson, Cassandra Goldsack, Lars Ostergaard, and Penny Hundleby Nee Sparrow

2

Cotton (Gossypium Hirsutum L.)

Keerti S. Rathore, Leanne M. Campbell, Shanna Sherwood, and Eugenia Nunes

3

Jatropha (Jatropha Curcas L.)

Devendra Kumar Maravi, Shamsher Alam, Purabi Mazumdar, Vaibhav V Goud, and Lingaraj Sahoo

4

Sesame (Sesamum Indicum L.)

Sonia Kapoor, Sanjay S. Parmar, Manju Yadav, Darshna Chaudhary, Manish Sainger, Ranjana Jaiwal,  and Pawan K. Jaiwal

5

Sunflower (Helianthus Annuus L.)

Laura M. Radonic, Dalia M. Lewi, Nilda E. López, H. Esteban Hopp, Alejandro S. Escandón, and Marisa López Bilbao

Part II

Root Plants

6

Carrot (Daucus Carota L.)

Owen S. D. Wally and Zamir K. Punja

7

Cassava (Manihot Esculenta Cranz)

Simon E. Bull

8

Potato (Solanum Tuberosum L.)

Martha L. Orozco-Cárdenas, Venkateswari J. Chetty, and Javier Narváez-Vásquez

9

Taro (Colocasia Esculenta (L.) Schott)

Xiaoling He, Susan C Miyasaka, Maureen MM Fitch, and Yun J Zhu

Part III

Nuts And Fruits

10

Apricot (Prunus Armeniaca L.)

César Petri, Nuria Alburquerque and Lorenzo Burgos

11

Blueberry (Vaccinium Corymbosum L.)

Guo-Qing Song

12

Cherry

Guo-Qing Song

13

Chestnut, American (Castanea Dentata (Marsh.) Borkh.)

Charles A. Maynard, Linda D. Mcguigan, Allison D. Oakes, Bo Zhang, Andrew E. Newhouse, Lilibeth C. Northern, Allison M. Chartrand, Logan R. Will, Kathleen M. Baier, and William A. Powell

14

Chestnut, European (Castanea Sativa)

Elena Corredoira, Silvia Valladares, Ana M. Vieitez, and Antonio Ballester

15

Grapevine (Vitis Vinifera L.)

Laurent Torregrosa, Sandrine Vialet, Angélique Adivèze, Pat Iocco-Corena, and Mark R. Thomas

16

Melon (Cucumis Melo)

Satoko Nonaka and  Hiroshi Ezura

17

Peach (Prunus Persica L.)

Silvia Sabbadini, Tiziana Pandolfini, Luca Girolomini, Barbara Molesini, and Oriano Navacchi

18

Strawberry (Fragaria X Ananassa)

Roberto Cappelletti, Silvia Sabbadinim, and Bruno Mezzetti

19

Walnut (Juglans)

Charles A. Leslie, Sriema L.Walawage, Sandra L. Uratsu, Gale Mcgranahan, and Abhaya M. Dandekar

Part IV

Tropic Plants

20

Citrus Transformation Using Juvenile Tissue Explants

Vladimir Orbović and Jude W. Grosser

21

Citrus Transformation Using Mature Tissue Explants

Vladimir Orbović, Alka Shankar, Michael E. Peeples, Calvin Hubbard, and Janice Zale

22

Coffee (Coffea Arabica L.)

Eveline Déchamp, Jean-Christophe Breitler, Thierry Leroy, and Hervé Etienne

23

Pineapple [Ananas Comosus (L.) Merr.]

Gaurab Gangopadhyay and Kalyan K Mukherjee

24

Sugarcane (Saccharum Spp. Hybrids)

Hao Wu and Fredy Altpeter

Part V

Other Important Plants

25

Hemp (Cannabis Sativa L.)

Mistianne Feeney and Zamir K. Punja

26

Orchids (Cymbidium Spp., Oncidium And Phalaenopsis)

Chia-Wen Li, Chia-Hui Liao, Xia Huang, and Ming-Tsair Chan

27

Poinsettia (Euphorbia Pulcherrima Willd. Ex Klotzsch)

M Ashraful Islam, Tage Thorstensen, and Jihong Liu Clarke

28

Populus Trichocarpa

Quanzi Li, Ting-Feng Yeh, Chenming Yang, Jingyuan Song, Zenn-Zong Chen, Ronald R. Sederoff, and Vincent L. Chiang

29

Tall Fescue (Festuca Arundinacea Schreb.)

Yaxin Ge and Zeng-Yu Wang

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts