Plant Mineral Nutrients, 2013 Methods and Protocols Methods in Molecular Biology Series, Vol. 953

The study of plant mineral nutrition has both academic and applied aspects to it. Today, research into plant mineral nutrition is more pertinent than ever in the face of a growing world population and the increasing need for sustainable agriculture. In Plant Mineral Nutrients: Methods and Protocols, expert researchers in the field detail a comprehensive collection of methodologies that are routinely used in plant mineral nutrition research. These include methods and protocols for plant growth parameters, ion contents and composition, soil analyses, flux measurements and the use of public facilities for high throughput analyses. Written in the highly successful Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Mineral Nutrients: Methods and Protocols seeks to aid scientist in the further study of into plants and their mineral nutrients.

Roles and Functions of Plant Mineral Nutrients.- Plant Growth and Cultivation.- Protocols for Growing Plant Symbioses; Mycorrhiza.-Protocols For Growing Plant Symbioses; Hizobia.- Plant Cell Suspension Cultures.- Soil Analysis using Visible and Near Infrared Spectroscopy.- Mineral Composition Analysis: Measuring Anion Uptake and Anion Concentrations in Plant Tissues.- Multi-element Plant Tissue Analysis using ICP Spectrometry.- Mapping Element Distributions in Plant Tissues using Synchrotron X-ray Fluorescence Techniques.- Flux Measurements of Cations using Radioactive Tracers.- Ion Flux Measurements using the MIFE Technique.-Sampling and Analysis of Phloem Sap.- Methods for Xylem Sap Collection.-Plant Single Cell Sampling.- Measurements of Cytosolic Ion Concentrations in Live Cells.- Real Time Measurement of Cytoplasmic Ions with Ion Selective Microelectrodes.- Large Scale Plant Ionomics.- Applications of High-throughput Plant Phenotyping to Study Nutrient use Efficiency.