Purinergic Signaling, 1st ed. 2020 Methods and Protocols Methods in Molecular Biology Series, Vol. 2041
Coordonnateur : Pelegrín Pablo
This volume aims to cover all major methodological aspects of research into purinergic signaling and to provide a foundation for studying them at molecular, biochemical, pharmacological, and physiological levels. Chapters guide readers through current knock-out and knock-in mouse models, in silico modeling, knock down purinoceptor expression, bioluminiscence resonance energy transfer, enzyme-based biosensors, recording P2X receptor electrophysiology, controlling P2X receptors by optogenetics, inflammasome activation, leukocyte migration, and cell adhesion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Purinergic Signaling: Methods and Protocols will provide a sound basis for molecular, cellular, and physiological research into purinergic signaling in health and disease and will spark interest in this fascinating signaling process among researchers in many different and unrelated disciplines.
1. Introduction to Purinergic Signaling
Geoffrey Burnstock
2. Knock-out and Knock-in Mouse Models to Study Purinergic Signaling
Robin M. H. Rumney and Dariusz C. Górecki
3. Agonists and Antagonists for Purinergic Receptors
Christa E. Müller and Vigneshwaran Namasivayam
4. Homology Modelling of P2X Receptors
Anastasios Stavrou, Sudad Dayl, and Ralf Schmid
5. Using RNA Interference for Purinoceptor Knock Down in vivo
Rebeca Padrão Amorim, Iscia Teresinha Lopes Cendes, and Maria Jose da Silva Fernandes
6. Developmental Expression of Ectonucleotidase and Purinergic Receptors Detection by Whole Mount in situ hybridization in Xenopus Embryos
Camille Blanchard and Karine Massé
7. Histochemical Approach for Simultaneous Detection of Ecto-nucleotidase and Alkaline Phosphatase Activities in Tissues
Karolina Losenkova, Marius Paul, Heikki Irjala, Sirpa Jalkanen, and Gennady G. Yegutkin
8. Flow Cytometry of Membrane Purinoreceptors
Nicole Schwarz, Marten Junge, Friedrich Haag, and Friedrich Koch-Nolte
9. Studying Purinoceptor Cell-surface Expression by Protein Biotinylation
Mark T Young
10. Multimeric Ionotropic Purinoceptor Detection by Protein Cross-linking
Vincent Compan and François Rassendren
11. Multimeric Purinoceptor Detection by Bioluminiscence Resonance Energy Transfer
Vincent Compan and François Rassendren
12. Application of Fluorescent Purinoceptor Antagonists for Bioluminescence Resonance Energy Transfer Assays and Fluorescent Microscopy
Mark Soave, Joëlle Goulding, Robert Markus, Stephen J Hill, and Leigh A Stoddart
13. Detection of Extracellular ATP in the Tumor Microenvironment, using the pmeLUC Biosensor
Elena De Marchi, Elisa Orioli, Anna Pegoraro, Elena Adinolfi, and Francesco Di Virgilio
14. Using Amperometric, Enzyme-Based Biosensors for Performing Longitudinal Measurements of Extracellular adenosine 5-triphosphate in the Mouse
Edward Beamer and Tobias Engel
15. Fluorescent Labeling and Quantification of Vesicular ATP Release using Live Cell Imaging
Kirstan A. Vessey, Tracy Ho, Andrew I. Jobling, Yao A. Wang, and Erica L. Fletcher
16. Using FRET-based Fluorescent Sensors to Monitor Cytosolic and Membrane-proximal Extracellular ATP levels Klaus E. Kaschubowski, Axel E. Kraft, Viacheslav O. Nikolaev, and Friedrich Haag
17. ATP Measurement in Cerebrospinal Fluid Using a Microplate Reader
Laura de Diego-García, Álvaro Sebastián-Serrano, Carolina Bianchi, Caterina Di Lauro, and Miguel Díaz-Hernández
18. P2X Electrophysiology and Surface Trafficking in Xenopus oocytes
Eléonore Bertin, Audrey Martinez, and Eric Boué-Grabot
19. Heterologous Expression and Patch-clamp Recording of P2X Receptors in HEK293 Cells
Lin-Hua Jiang and Sébastien Roger
20. Recording P2X Receptors Using Whole Cell Patch Clamp from Native Monocytes and Macrophages
Leanne Stokes
21. Automated Planar Patch-clamp Recording of P2X Receptors
Carol J. Milligan and Lin-Hua Jiang
22. Controlling Engineered P2X Receptors with Light
Benjamin N. Atkinson, Vijay Chudasama, and Liam E. Browne
23. Intracellular Calcium Recording after Purinoceptor Activation Using a Video-microscopy Equipment
Maria Teresa Miras-Portugal, Felipe Ortega, Javier Gualix, Raquel Perez-Sen, Esmerilda G. Delicado, and Rosa Gomez-Villafuertes
24. Assays to Measure Purinoceptor Pore Dilation
Ben J. Gu, Pavan Avula and James S. Wiley
25. Detection of Inflammasome Activation by P2X7 Purinoceptor Activation by Determining ASC Oligomerization
Helios Martínez-Banaclocha and Pablo Pelegrín
26. Measuring Leukocyte Migration to Nucleotides
Taylor J. Moon and Michael R. Elliott
27. Assessment of Cell Adhesion after Purinoceptor Activation
Juan J. Martínez-García and Pablo Pelegrín
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Date de parution : 10-2020
Ouvrage de 360 p.
17.8x25.4 cm
Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).
Prix indicatif 116,04 €
Ajouter au panierDate de parution : 10-2019
Ouvrage de 360 p.
17.8x25.4 cm
Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).
Prix indicatif 158,24 €
Ajouter au panier