ErbB Receptor Signaling, 1st ed. 2017 Methods and Protocols Methods in Molecular Biology Series, Vol. 1652

This volume contains protocols specifically designed for studying cell signaling mediated by ErbB receptors, all applicable to the study of a broad range of ErbB receptor-mediated signaling from basic research to clinic applications, from cultured cells to various animal models and primary cancer cells from patients. After some overviews, the book continues by covering common protocols for studying various aspects of ErbB receptor-mediated cell signaling, newly-developed methods in biomedical research that are also widely-used in the study of ErbB receptor signaling, epidermal growth factor receptor (EGFR) signaling in Drosophila, as well as protocols for studying ErbB receptor signaling in various animal model systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

ErbB Receptors and Cancer.- New Insights from Drosophila into the Regulation of EGFR Signaling.- C. elegans Vulva Induction: An In Vivo Model to Study Epidermal Growth Factor Receptor Signaling and Trafficking.- Targeting HER2 in Advanced Breast Cancer.- Methods to Investigate EGFR Ubiquitination.- Dimerization Assessment of Epithelial Growth Factor Family of Receptor Tyrosine Kinases by Using Cross-Linking Reagent.- Application of Immunofluorescence Staining to Study ErbB Family of Receptor Tyrosine Kinases.- Activation of Endosome-Associated Inert EGF Receptor Following Internalization.- Two-Pulse Endosomal Stimulation of Receptor Tyrosine Kinases Induces Cell Proliferation.- Study of EGFR Signalling/Endocytosis by Site-Directed Mutagenesis.- Using Percoll Gradient Fractionation to Study the Endocytic Trafficking of the EGFR.- Analysis of Epidermal Growth Factor Receptor-Induced Cell Motility by Wound Healing Assay.- Cell Cycle Synchronization of HeLa Cells to Assay EGFR Pathway Activation.- Analysis of Constitutive EGFR Signaling Regulating IRF3 Transcriptional Activity in Cancer Cells.- Measurement of Epidermal Growth Factor Receptor-Derived Signals Within Plasma Membrane Clathrin Structures.- Studying Non-Proliferative Roles for Egfr Signaling in Tissue Morphogenesis Using Dorsal Closure of the Drosophila Embryo.- Analysis of Epithelial-Mesenchymal Transition Induced by Overexpression of Twist.- Assessment of Specificity of an Adenovirus Targeted to HER3/4.- Isolation of Human Mesenchymal Stem Cells for Studying ErbB Receptor Signaling.

Includes cutting-edge methods and protocols for the study of ErbB receptor signaling Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras