Protein Complex Assembly, Softcover reprint of the original 1st ed. 2018 Methods and Protocols Methods in Molecular Biology Series, Vol. 1764
This volume details the importance of multiple experimental techniques and computational methods needed to obtain the comprehensive picture of protein complex structure, dynamics and assembly afforded by the emerging field of integrative structural biology. Chapters guide readers through the broad spectrum of approaches required for a complete representation of protein complexes, including expression and purification, experimental characterization of structure and assembly, and computational methods for identifying protein complexes and modelling their assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Protein Complex Assembly: Methods and Protocols aims to ensure successful results in the further study of this vital field.
PART I – Experimental methods
1. Experimental Characterisation Of Protein Complex Structure, Dynamics, and Assembly
Jonathan N. Wells and Joseph A. Marsh
2. High-Throughput Electron Cryo-Tomography of Protein Complexes and their Assembly
Louie D. Henderson and Morgan Beeby
3. Preparation of Tunable Microchips to Visualize Native Protein Complexes for Single Particle Electron Microscopy
Brian L. Gilmore, A. Cameron Varano, William Dearnaley, Yanping Liang, Bridget C. Marcinkowski, Madeline J. Dukes, and Deborah F. Kelly
4. Time-resolved Cryo-Electron Microscopy Using a Microfluidic Chip
Sandip Kaledhonkar, Ziao Fu, Howard White and Joachim Frank
5. Characterizing Protein-protein Interactions using Solution NMR
Spectroscopy
Jose Luis Ortega-Roldan, Martin Blackledge and Malene Ringkjøbing Jensen
6. Reconstitution of Isotopically Labeled Ribosomal Protein L29 in the 50S Large Ribosomal Subunit For Solution-state and Solid-state NMR
Emeline Barbet-Massin, Eli van der Sluis, Joanna Musial, Roland Beckmann, and Bernd Reif
7. Characterizing Protein-Protein Interactions using Deep Sequencing Coupled to Yeast Surface DisplayAngelica V. Medina-Cucurella and Timothy A. Whitehead
8. Structurally Guided In Vivo Crosslinking
Johanna C. Scheinost and Thomas G. Gligoris
9. Characterising Intact Macromolecular Complexes using Native Mass Spectrometry Elisabetta Boeri Erba, Luca Signor, Mizar F. Oliva, Fabienne Hans, and Carlo Petosa
10. Hydrogen Deuterium Exchange Mass Spectrometry to Study Protein ComplexesBrent A. Kochert, Roxana E. Iacob, Thomas E. Wales, Alexandros Makriyannis, and John R. Engen
11. Structural Analysis of Protein Complexes by Cross-linking and Mass-spectrometry
Moriya Slavin and Nir Kalisman
12. Global Characterization of Protein Complexes by Biochemical Purification-mass Spectrometry (BP/MS)
Reza Pourhaghighi and Andrew Emili
13. Proteomic Profiling of Integrin Adhesion Complex Assembly
Adam Byron
14. Dual-Color and 3D Super-Resolution Microscopy of Multi-protein Assemblies
Philipp Hoess, Markus Mund, Manuel Reitberger and Jonas Ries
15. Correlative 3D Structured Illumination Microscopy and Single Molecular Localisation Microscopy for Imaging Cancer Invasion
Shannon J.L. Pinnington, John F. Marshall and Ann P. Wheeler16. Observing the Assembly of Protein Complexes In Living Eukaryotic Cells In Super-resolution using refSOFI
Fabian Hertel, Gary C.H. Mo, Peter Dedecker and Jin Zhang
17. Detecting Purinosome Metabolon Formation with Fluorescence Microscopy
Anthony M. Pedley and Stephen J. Benkovic
18. Analysis of Bacterial pilus Assembly by Shearing and Immunofluorescence Microscopy
Areli Luna-Rico, Jenny-Lee Thomassin and Olivera Francetic
19. Expression, Purification and Assembly of Archaellum Subcomplexes of Sulfolobus Acidocaldarius
Paushali Chaudhury, Patrick Tripp, and Sonja-Verena Albers
20. Unstable Protein Purification through the Formation of Stable Complexes
Sylvia Eiler, Nicolas Levy, Benoit Maillot, Julien Batisse, Karine Pradeau Aubreton, Oyindamola Oladosu, and Marc Ruff
21. Expressing Multi-subunit Complexes using biGBac
Florian Weissmann and Jan-Michael Peters
PART II – Computational Methods
22. Computational Modelling of Protein Complex Structure and Assembly
Jonathan N. Wells, L. Therese Bergendahl, and Joseph A. Marsh
23. Inferring and using Protein Quaternary Structure Information from Crystallographic Data Sucharita Dey and Emmanuel D. Levy
24. Searching and Extracting Data from the EMBL-EBI Complex PortalBirgit H. M. Meldal and Sandra Orchard
25. Automated Computational Inference of Multi-protein Assemblies from Biochemical Co-Purification DataFlorian Goebels, Lucas Hu, Gary Bader, and Andrew Emili
26. A Multi-scale Computational Model for Simulating the Kinetics of Protein Complex Assembly
Jiawen Chen and Yinghao Wu
27. Flexible Protein-protein Docking with SwarmDock
Iain H. Moal, Raphael A.G. Chaleil, and Paul A. Bates
28. Protein-protein Docking using Evolutionary Information
Aravindan Arun Nadaradjane, Raphael Guerois, and Jessica Andreani
29. Modeling Structure and Dynamics of Protein Complexes with SAXS Profiles
Dina Schneidman-Duhovny and Michal Hammel
30. Modeling the Structure of Helical Assemblies with Experimental Constraints in Rosetta Ingemar André
31. Selecting Conformational Ensembles Using Residual Electron and Anomalous Density (READ)Loïc Salmon, Logan S Ahlstrom, James C.A. Bardwell, and Scott Horowitz
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Date de parution : 12-2019
Ouvrage de 506 p.
17.8x25.4 cm
Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).
Prix indicatif 126,59 €
Ajouter au panierDate de parution : 04-2018
Ouvrage de 506 p.
17.8x25.4 cm
Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).
Prix indicatif 105,49 €
Ajouter au panier