Protein Self-Assembly, 1st ed. 2019 Methods and Protocols Methods in Molecular Biology Series, Vol. 2039
This volume explores experimental and computational approaches to measuring the most widely studied protein assemblies, including condensed liquid phases, aggregates, and crystals. The chapters in this book are organized into three parts: Part One looks at the techniques used to measure protein-protein interactions and equilibrium protein phases in dilute and concentrated protein solutions; Part Two describes methods to measure kinetics of aggregation and to characterize the assembled state; and Part Three details several different computational approaches that are currently used to help researchers understand protein self-assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge, Protein Self-Assembly: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field.
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Date de parution : 08-2020
Ouvrage de 266 p.
17.8x25.4 cm
Date de parution : 07-2019
Ouvrage de 266 p.
17.8x25.4 cm
Thème de Protein Self-Assembly :
Mots-clés :
Globular Proteins; protein unfolding; mesophases; microscopy; capillary electrophoresis