Solanum tuberosum, 1st ed. 2021 Methods and Protocols Methods in Molecular Biology Series, Vol. 2354

Importance of Potato as a Crop and Practical Approaches to Potato Breeding.- Cryopreservation of Potato Shoot Tips for Long Term Storage.- RNA Sequencing Analyses for Deciphering Potato Molecular Response.- Yeast-2-hybrid Screening for Identification of Protein-Protein Interactions in Solanum tuberosum.- Potato as a Model for Field Trials with Modified Gene Functions in Research And Translational Experiments.- DAP-seq Identification of Transcription Factor Binding Sites in Potato.- Mass Spectrometric Monitoring Of Plant Hormone Crosstalk During Biotic Stress Responses In Potato (Solanum tuberosum L.).- A Comprehensive Guide to Potato Transcriptome Assembly.- MapMan visualization of RNA-seq data using Mercator4 functional annotations.- Identification of Resistance Genes Using Diagnostic R-gene Enrichment Sequencing (dRenSeq).- Methodologies for Discovery and Quantitative Profiling of sRNAs in Potato.- Co-expression for Genotype-Phenotype Function Annotation In Potato Research.- Computer Vision and Less Complex Image Analyses to Monitor Potato Traits In Fields.- Quantifying the contribution to virulence of Phytophthora infestans effectors in potato.- Identification of Solanum immune receptors by Bulked Segregant RNA-Seq and high-Throughput Recombinant Screening.- Gene editing in potato using CRISPR-Cas9 technology.- Gene downregulation in potato roots using Agrobacterium rhizogenes-mediated Transformation.- Molecular Detection of Ralstonia solanacearum to Facilitate Breeding for Resistance to Bacterial Wilt in Potato.- Towards the design of potato tolerant to abiotic stress.- Rapid loop-mediated isothermal amplification for detection of the Ralstonia solanacearum species complex bacteria in symptomatic potato tubers and plants.