Stem Cells and Tissue Repair (2^nd Ed., 2nd ed. 2020) Methods and Protocols Methods in Molecular Biology Series, Vol. 2155

Preface…
Table of Contents…
Contributing Authors…

1. Culturing and Manipulating Mouse Embryonic Stem Cells
Chrissa Kioussi

2. Generation of an Induced Pluripotent Stem Cell Line with the Constitutive EGFP Reporter 
Kiel T. Carson Butterfiel, Patrick S. McGrath, Chann Makara Han, Igor Kogut, and Ganna Bilousova

3. Engraftable Induced Pluripotent Stem Cell-Derived Neural Precursors for Brain Repair
Ourania Zygogianni, Georgia Kouroupi, Era Taoufik, and Rebecca Matsas

4. In Vitro Direct Reprogramming of Mouse and Human Astrocytes to Induced Neurons
Katerina Aravantinou-Fatorou and Dimitra Thomaidou

5. Preparation of Bioscaffolds Delivering Stem Cells for Neural Regeneration 
Li Yao and Ashley DeBrot

6. Improved Isolation of Human Vascular Wall-Resident Mesenchymal Stem Cells
Diana Klein

7. In Vitro Generation of Vascular Wall-Typical Mesenchymal Stem Cells (VW-MSC) from Murine Induced Pluripotent Stem Cells through VW-MSC Specific Gene Transfer
Jennifer Steens, Hannes Klump, and Diana Klein

8. Analysis of Tooth Innervation in Microfluidic Co-Culture Devices
Pierfrancesco Pagella and Thimios A. Mitsiadis

9. Peripheral Nerve Regeneration in a Novel Rat Model of Dysphagia
Kiyoshi Sakai, Takeshi Tsuruta, Junna Watanabe, Yukiko Sugimura, Kohei Sakaguchi, Wataru Katagiri, and Hideharu Hibi

0. Healing of Full Thickness Murine Skin Wounds Containing Nanofibers using Splints for Efficient Re-Epithelialization and to Avoid Contracture
Nilika Bhattacharya, Arup K. Indra, and Gitali Ganguli-Indra

11. Enrichment and Characterization of Human and Murine Pulmonary Mesenchymal Progenitor Cells (MPC)
Megan Summers, Karen Helm, and Susan M. Majka

12. Isolation and Culture of Quiescent Skeletal Muscle Satellite Cells 
Francisco Hernández-Torres, Lara Rodríguez-Outeiriño, and Amelia Aránega

13. Isolation, Culture, Cryopreservation and Identification of Bovine, Murine, and Human Spermatogonial Stem Cells 
Pedro M. Aponte

14. Long-Term Ex Vivo Expansion of Murine Spermatogonial Stem Cells in a Simple Serum-Free Medium
Hiroshi Kubota and Kazue Kakiuchi

15. Generating Kidney Organoids from Human Pluripotent Stem Cells Using Defined Conditions
Sara E. Howden and Melissa H. Little

16. Painting the Pancreas in Three Dimensions: Whole Mount Immunofluorescence Method
Maricela Maldonado, Jeffrey D. Serrill, and Hung-Ping Shih

17. Induction of Osteoblasts by Direct Reprogramming of Mouse Fibroblasts 
Hui Zhu and Joy Wu

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts