Reactive Oxygen Species, 1st ed. 2021 Methods and Protocols Methods in Molecular Biology Series, Vol. 2202
Coordonnateur : Espada Jesús
This volume provides a varied selection of current methods and molecular probes used to generate, image and quantify ROS production and lipid peroxidation. Chapters guide readers through methods and protocols on in silico modelling of ROS dynamics in biological systems and a breaking proposal. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Reactive Oxygen Species: Methods and Protocols aims to ensure successful results in the further study of this vital field.
1. Introduction to in silico Modeling to Study ROS Dynamics
Jana Schleicher
2. Assessment of ROS Production in Mitochondria of Live Cells
Plamena R. Angelova, Albena T. Dinkova-Kostova, and Andrey Y. Abramov
3. Measurement of Superoxide Production in Acute Hypoxia by Fixed-Cell Microscopy
Pablo Hernansanz-Agustíz, Carmen Choya-Foces, and Antonio Martínez-Ruiz
4. A Photodynamic Tool to Promote a Sustained, ROS-Dependent Growth of Human Hair Follicles in ex vivo Culture
María I. Calvo-Sánchez, Sandra Fernández-Martos, and Jesús Espada
5. Singlet Oxygen and Protochlorophyllide Detection in Arabidopsis thaliana
Liangsheng Wang and Tatjana Kleine
6. Characterization of oxidative lipidomics and autophagy induction in Chlamydomonas reinhardtii under abiotic stress
Cristina Ortega-Villasante, Ángel Barón-Sola, Margarita Toledo-Basantes, Flor Martínez, and Luis E. Hernández
7. Fluorescence Detection of Increased Reactive Oxygen Species Levels in Saccharomyces cerevisiae at the Diauxic Shift
Abhishek Sinha and Elah Pick
8. Flow Cytometry and Confocal Microscopy for ROS Evaluation in Fish and Human Spermatozoa
Vanesa Robles, Marta F. Riesco, Juan M. Martínez-Vázquez, and David G. Valcarce
9. Measurement of Reactive Oxygen Species In Semen Samples Using Chemiluminescence
Tânia R. Dias
10. Methods to Unravel Pathways of Reactive Oxygen Species in the Photodynamic Inactivation of Bacteria
Natalia S. Gsponer and Edgardo N. Durantini
11. Revealing ROS Production by Antibiotics and Photosensitizers in Biofilms. A Fluorescence Microscopy Approach
Sol R. Martínez and Andrés M. Durantini
12. EPR Spectroscopy as a Method for ROS Quantification in Skin
Silke B. Lohan, Daniela Ivanov, Nadine Schüle, Bastian Berger, Stephanie Albrecht, and Martina C. Meinke
13. Detection of Superoxide Radical in Adherent Living Cells by Electron Paramagnetic Resonance (EPR) Spectroscopy Using Cyclic Nitrones
Kahina Abbas, Nikola Babić, and Fabienne Peyrot
14. Singlet Oxygen Quantum Yield Determination Using Chemical Acceptors
Roger Bresolí-Obach, Joaquim Torra, Renzo P. Zanocco, Antonio L. Zanocco, and Santi Nonell
15. A General Method to Establish the Relative Efficiency of Different Sonosensitizers to Generate ROS for SDT
Viviana Grosso, Alejandro Senz, and Arnaldo T. Soltermann
16. Lipid Peroxidation Assay using BODIPY-phenylbutadiene Probes:
A Methodological Overview
Juan C. Stockert
17. Use of Fluorescent Probes to Detect ROS in Photodynamic Therapy
Sulbha K Sharma and Michael R Hamblin
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Date de parution : 08-2021
Ouvrage de 233 p.
17.8x25.4 cm
Date de parution : 08-2020
Ouvrage de 233 p.
17.8x25.4 cm
Thème de Reactive Oxygen Species :
Mots-clés :
fluorescent biosensors; microbial cells; Amplex Red; cell function; EPR spectroscopy
