Redox-Mediated Signal Transduction (2^nd Ed., 2nd ed. 2019) Methods and Protocols Methods in Molecular Biology Series, Vol. 1990

This volume explores the scope of the cellular redox analysis and the importance of not being limited by frequently changing and evolving technology. The chapters in this book cover a wide range of topics such as redox components in animal and plant cells and the role of reactive oxygen species, reactive nitrogen species, and hydrogen sulphide in cell signaling; measuring modifications using Flow Cytometry, ELISA assays, and Western blot analysis; measurement of oxidative stress in mitochondira and biological systems; and the use of the genetically encoded fluorescent probe HyPer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Cutting-edge and informative, Redox-Mediated Signal Transduction: Methods and Protocols, Second Edition is a valuable resource for both novice and expert researchers who want to expand their studies into new areas and new systems in the evolving redox field. 

Preface…

Table of Contents…

Contributing Authors…

1. The Role of Redox in Signal Transduction

John T. Hancock and Myra Conway

2. Methods for the Addition of Redox Compounds

John T. Hancock

3. Investigating ROS, RNS, and H2S Sensitive Signalling Proteins

Eleanor Williams, Matthew Whiteman, Mark E. Wood, Ian D. Wilson, Michael R. Ladomery, Joel Allainguillaume, Tihana Teklic, Miro Lisjak, and John T. Hancock

Kosha Mehta and Vinood B. Patel

5. Using Flow Cytometry to Detect and measure Intracellular Thiol Redox Status in Viable T Cells from Heterogeneous Populations

Alex J. Wadley, Rhys G. Morgan, Richard L. Darley, Paul S. Hole, and Steven J. Coles

6. Detection of S-Nitrosation and S-Glutathionylation of the Human Branched-Chain Aminotransferase Proteins

Thomas E. Forshaw and Myra E. Conway

7. Visualization of Intracellular Hydrogen Peroxide produced by DAO with Genetically Encoded Fluorescent Probe HyPer in NIH-3T3 Cells

Yulia G. Ermakova and Vsevolod V. Belousov

8. Applications of Electron Paramagnetic Resonance (EPR) Spectroscopy to the Study of Oxidative Stress in Biological Systems

Simon Jackson

9. Extracellular and Intracellular NO Detection in Plants by Diaminofluoresceins

Neidiquele M. Silveira, Eduardo C. Machado, and Rafael V. Ribeiro

10. Working with Hypoxia

Elizabeth Bowler and Michael Ladomery

11. Predicting the Effects of Low Dose-Rate Ionising Radiation on Redox Potential in Plant Cells

Nicol Caplin and Neil Willey

12. Thioredoxin-1 PEGyletion as an In Vitro Method for Drug Target Identification 

Jolanta Skalska

13. Redox-Regulated, Targeted Affinity Isolation of NADH-Dependent Protein Interactions with the Branched Chain Aminotransferase Proteins

Maya E.L. Hindy and Myra E. Conway

14. Analysis of Redox Relationships in the Plant Cell Cycle: Determination of Ascorbate, Glutathione and Poly(ADPribose)polymerase (PARP) in Plant Cell Cultures

Christine H. Foyer, Till K. Pellny, Vittoria Locato, Jonathon Hull, and Laura De Gara

15. Equations to Support Redox Experimentation

John T. Hancock and Matthew Whiteman