Protein Gel Detection and Imaging, Softcover reprint of the original 1st ed. 2018 Methods and Protocols Methods in Molecular Biology Series, Vol. 1853

Preface…

Table of Contents…

Contributing Authors…

1. Protein Stains and Applications

Pazhani Sundaram

2. The Roles of Acetic Acid and Methanol during Fixing and Staining Proteins in a SDS Polyacrylamide Electrophoresis Gel

J.P. Dean Goldring

3. Multicolored Prestained Standard Protein Marker Generation using a Variety of Remazol Dyes for Easy Visualization of Protein Bands during SDS-PAGE

Gaurav Kumar

4. Coomassie-Brilliant Blue Staining of Polyacrylamide Gels

Claudia Arndt, Stefanie Koristka, Anja Feldmann, Ralf Bergmann, and Michael Bachmann

5. A Simple, Time-Saving Dye Staining of Proteins in Sodium Dodecyl Sulfate Polyacrylamide Gel using Coomassie Blue

Wei-hua Dong, Fang Wang, Jun-he Zhang, Yan-sheng Zhou, Ling-ye Zhang, and Tian-yun Wang

6. Application of Heat to Quickly Stain and Destain Proteins Stained with Coomassie Blue

Biji T. Kurien and R. Hal Scofield

7. Silver Staining Techniques of Polyacrylamide Gels

Nicole Berndt, Rolf Bergmann, Claudia Arndt, Stefanie Koristka, and Michael Bachmann

8. Counterion Dye Staining of Proteins in One- and Two-Dimensional Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Tryptic Gel Digestion of Stained Protein for Mass Spectrometry

Sun-Yong Hwang and Jung-Kap Choi

9. Detection of Phosphoproteins in Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis using 8-Quinolinol Stain

Sun-Yong Hwang, Xu Wang, and Jung-Kap Choi

10. Microwave Assisted Protein Staining, De-Staining and In-Gel/In-Solution Digestion of Proteins

Jennie R. Lill and Victor J. Nesatyy

11. Fluorescent Staining of Gels

Engelbert Buxbaum

12. A Single-Step Simultaneous Protein Staining Procedure for Polyacrylamide Gels and Nitrocellulose Membranes by Alta during Western Blot Analysis

Jayanta K. Pal, Sunil K. Berwal, and Rupali N. Soni

Na Na and Jin Ouyang

14. Detection of Glycoproteins in Polyacrylamide Gels using Pro-Q Emerald 300 Dye, A Fluorescent Periodate Schiff-Base Stain

Padmaja Mehta-D’souza

15. Curcumin/Turmeric as an Environment-Friendly Protein Gel Stain

Biji T. Kurien, Yaser Dorri, and R. Hal Scofield

16. Detection of Multiple Enzymes in Fermentation Broth using Single PAGE Analysis

Divakar, K., Deepa Arul Priya, J, Panneer Selvam, G., Suryia Prabha, M., Ashwin Kannan, Nandhini Devi, G., and Pennathur Gautam

17. Revisit of Imidazole-Zinc Reverse Stain for Protein Polyacrylamide Gel Electrophoresis

Han-Min Chen

18. A One-Step Staining Protocol for In-Gel Fluorescent Visualization of Proteins

Jelena Bogdanović Pristov and Ivan Spasojević

19. Tem Minute stain to Detect Proteins in Polyacrylamide Electrophoresis Gels with Direct red 81 and Amido Black

J.P. Dean Goldring and Robert G.E. Krause

20. In-Gel Protein Phosphatase Assay using Fluorogenic Substrates

Isamu Kameshita, Noriyuki Sueyoshi, and atsuhiko Ishida

21. Detection of Proteins in Polyacrylamide Gels via Pre-Labeling by Isatoic Anhydride

Kazem Asadollahi, Saharnaz Rafiee, and Gholamhossein Riazi

22. Fluorescent Protein Visualization Immediately after Gel Electrophoresis using an In-Gel Trichloroethanol Photoreaction with Tryptophan

Carol L. Ladner-Keay, Raymond J. Turner, and Robert A. Edwards

23. Direct Immunodetection of Antigens within the Precast Polyacrylamide Gel

Surbhi Desai, Boguslawa R. Dworecki, and Marie C. Nlend

24. Zymographic Determination of Intrinsic Specific Activity of Oxidases in Presence of Interfering Proteins

Tien Canh Le, Mircea Alexandru Mateescu, Samaneh Ahmadifar, Lucia Marcocci, and Paola Pietrangeli

25. A Simple Method for Detecting Phosphorylation of Proteins by using ZN2+ -Phos-tag SDS-PAGE at Neutral pH

Gaurav Kumar

Gaurav Kumar

27. Heat/Pressure Treatment with Detergents Significantly Increases Curcumin Solubility and Stability: Its use as an Environment-Friendly Protein Gel Stain

Biji T. Kurien, Rohit Thomas, Adam Payne, and R. Hal Scofield

Gaurav Kumar

29. Destaining Coomassie Brilliant Blue-Stained Sodium Dodecyl Sulfate Polyacrylamide Protein Gels using a Household Detergent

Rachna Aggarwal and Biji T. Kurien

30. Paper Adsorbents Remove Coomassie Blue from Gel Destain and used Gel Stain in an Environment-Friendly Manner

Yaser Dorri and Biji T. Kurien

31. Gel Drying Methods

Anja Felmann, Nicole Berndt, Ralf Bergmann, and Michael Bachmann

32. Stained Gels can be Stored for Several Months in Non-Sealed Polyethylene Bags

Biji T. Kurien and R. Hal Scofield

33. Radiolabeling and Analysis of Labeled Proteins

Nicole Berndt, Ralf Bergmann, and Michael Bachmann

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts