Electrophoretic Separation of Proteins, 1st ed. 2019 Methods and Protocols Methods in Molecular Biology Series, Vol. 1855

Preface…

Table of Contents…

Contributing Authors…

1. How It All Began: A Personal History of Gel-Electrophoresis

Oliver Smithies

2. Introduction to Protein Electrophoresis

Pothur R. Srinivas

3. Concentrating Proteins by Salt, Polyethylene Glycol, Solvent, SDS Precipitation, Three Phase Partitioning, Dialysis, Centrifugation, Ultrafiltration, Lyophilisation, Affinity Chromatography, Immunoprecipitation or Increased Temperature for Protein Isolation, Drug Interaction, and Proteomic and Peptidomic Evaluation

J.P. Dean Goldring

4. Lysis Buffer Choices are Key Considerations to Ensure Effective Sample Solubilization for Protein Electrophoresis

Ewa I. Miskiewicz and Daniel J. MacPhee

5. The Cydex Blue Assay: A One Step Protein Assay for Samples Prior to SDS Electrophoresis

Thierry Rabilloud

6. Cellulose-Acetate Electrophoresis of Hemoglobin

Ramesh Kumar and Wilbert A. Derbigny

7. Native Polyacrylamide gels

Claudia Arndt, Stefanie Koristka, Anja Feldmann, and Michael Bachmann

8. Isoelectric Focusing on Non-Denaturing Gels

Biji T. Kurien and R. Hal Scofield

9. Determination of protein Molecular weights on SDS-PAGE

Hiroyuki Matsumoto, Hisao Haniu, and Naoka Komori

10. Two-Dimensional Gel Electrophoresis by Glass Tube-Based IEF and SDS-PAGE

Hiroyuki Matsumoto, Hisao Haniu, Biji T. Kurien, and Maoka Komori

11. Cationic Electrophoresis

Engelbert Buxbaum

12. Two-Dimensional Gel Electrophoresis with Immobilized pH Gradients

Bre’Ana Byrd and Huyen Tran

13. SARCOSYL-PAGE: Optimized Protocols for the Separation and Immunological Detection of PEGylated Proteins

Christian Reichel, Günter Gmeiner, Philipp Reihlen, Mario Thevis, and Wilhelm Schänzer

14. Tricine-SDS-PAGE

Syed R. Haider, Helen J. Reid, and Barry L. Sharp

15. Analysis of Protein Glycation using Phenylboronate Acrylamide Gel Electrophoresis

Marta P. Pereira Morais, Omar Kassaar, Stephen E. Flower, Robert J. Williams, Tony D. James, and Jean M. H. Van den Elsen

16. Immunofixation Electrophoresis for Identification of Proteins and Specific Antibodies

Gyorgy Csako

17. Electrophoretic Separation of Very Large Molecular Weight Proteins in SDS-Agarose

Marion L. Greaser and Chad M. Warren

18. Increase in Local Protein Concentration by Field-Inversion Gel Electrophoresis

Henghang Tsai and Hon-chiu Eastwood Leung

19. Two-Dimensional Difference Gel Electrophoresis

Malachi Blundon, Vinitha Ganesan, Brendan Redler, Phu T. Van, and Jonathan S. Minden

20. Immunoelectrophoresis: A Method with Many Faces

Gyorgy Csako

21. Tris-Acetate Polyacrylamide Gradient Gels for the Simultaneous Electrophoretic Analysis of Proteins if Very High and Low Molecular Mass

Monica Cubillos-Rojas, Fabiola Amair-Pinedo, Irantzu Tato, Ramon Bartrons, Francesc Ventura, and Jose Luis Rosa

22. Diagnoal Electrophoresis for Detection of Proteins Involved in Disulfide Bonds

Ronald Saraswat and Brian McDonagh

23. Identification of Proteins on Archived 2D Gels

Hiroyuki Matsumoto, Nobuaki Takemori, and Naoka Komori

24. Two-Dimensional Gel Electrophoresis: Vertical Isoelectric Focusing

Yaser Dorri

25. A Foodomics Approach: CE-MS for Comparative Metabolomics of Colon Cancer Cells Treated with Dietary Polyphenols

Mustafa Çelebier, Clara Ibáñez, Carolina Simó, and Alejandro Cifuentes

26. Characterization of New Cyclic D, L – α – Alternate Amino Acid Peptides by Capillary Electrophoresis Coupled to Electrospray Ionization Mass Spectrometry

María Dámaris Cortez-Díaz, Fanny d’Orlyé, and Anne Varenne

27. “Microchip Electrophoresis”, with Respect to “Profiling of Aβ Peptides in the Cerebrospinal Fluid of Patients with Alzheier’s Disease”  

Mohamad Reza Mohamadi, Romain Verpillot, Myriam Taverna, Markus Otto, and Jean-Louis Viovy

28. Measuring Low-Picomolar Apparent Binding Affinities by Minigel Electrophoretic Mobility Shift

Karen A. Lewis, Sarah E. Altschuler, and Deborah S. Wuttke

29. Identification of Proteins Interacting with Single Nucleotide Polymorphisms (SNPs) by DNA Pull-Down Assay

Bhupinder Singh and Swapan K. Nath

30. Horizontal Agarose Gel Mobility Shift Assay for Protein-RNA Complexes

Jennifer A. Ream, L. Kevin Lewis, and Karen A. Lewis

31. Applications of Immobilized Metal affinity Electrophoresis

Bao-Shiang Lee, Lasanthi P. Jayathilaka, Jin-Sheng Huang, and Shalini Gupta

32. Isoelectric Focusing in Agarose Gel for Detection of Oligoclonal Bands in Cerebrospinal and Other Biological Fluids

Gyorgy Csako

33. A Combined Free Flow Electrophoresis and DIGE Approach to Compare Proteins in Complex Biological Samples

Kim Y.C. Fung, Chris Cursaro, Tanya Lewanowitsch, Leah Cosgrove, and Peter Hoffmann

34. SDS PAGE for 35S Immunoprecipitation and Immunoprecipitation Western Blotting

Edward P. Trieu and Ira N. Targoff

35. A Multichannel Gel Electrophoresis and Continuous Fraction Collection Apparatus for High-Throughput Protein Separation and Characterization

Ming Dong, Megan Choi, Mark D. Biggin, and Jian Jin

36. Cell Surface Protein Biotinylation for SDS-PAGE Analysis

Giuliano Elia

37. Isolation of Proteins from Polyacrylamide Gels

Stefanie Koristka, Claudia Arndt, Ralf Bergmann, and Michael Bachmann

38. Continuous Elution SDS-PAGE with a Modified Standard Gel Apparatus to Separate and Isolate an Array of Proteins from Complex Mixtures

Robert G.E. Krause and J.P. Dean Goldring

39. Protein Extraction from Gels: A Brief Review

Biji T. Kurien, Rachna Aggarwal, and R. Hal Scofield

40. Gel Absorption-Based Sample Preparation Method for Shotgun Analysis of Membrane Proteome

Xianchun Wang and Songping Liang

41. Ultra-Rapid Sodium Dodecyl Sulfate Polyacrylamide Mini-Gel Electrophoresis

Rohit Thomas and Biji T. Kurien

James D. Fesmire

43. Single-Cell High Resolution Detection and Quantification of Protein Isoforms Differing by a Single Charge Unit

Kristi A. Koelsch

44. Artifacts and Common Errors in Protein Gel Electrophoresis

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts