Plant Cell Biology Methods in Cell Biology Series

Plant Cell Biology, volume 160 in "Methods in Cell Biology", includes chapters on modern experimental procedures and applications developed for research in the broad area of plant cell biology. Topics covered in this volume include techniques for imaging and analyzing membrane dynamics and movement across membranes; cell wall composition, structure and mechanics; cytoskeleton dynamics and organization; cell development; ion channel physiology; cell mechanics; and methods related to quantifying cell morphogenesis.

1. Imaging secretory events in plant cells Chunhua Zhang 2. 3D electron microscopy of plant membrane systems Marisa Otegui 3. Methods for studying protein targeting to and within chloroplasts Steve Theg 4. Single channel measurements of plant mechanosensitive ion channels Elizabeth Haswell 5. Quantitative analysis of cytoplasmic streaming Andreas Nebenführ 6. Imaging movement through plasmodesmata Jung-Youn Lee 7. Biochemical analysis of cell wall polymers Jenny C. Mortimer 8. Expression, biochemistry and structure of plant glycosyltransferases Breeanna Rae Urbanowicz 9. Studying cell wall mechanics using atomic force microscopy Sarah Robinson 10. Staining and automated image analysis of callose in the plant cell wall Antje Heese 11. Tracking of cellulose synthase complexes Charles Anderson 12. Glycosylation of cell wall proteins Cora Macalister 13. Super-resolution imaging of microtubules in Medicago sativa Jozef Šamaj 14. Live imaging of microtubules in petal conical cells Deshu Lin 15. Purification of plant tubulins for in vitro studies Takashi Hashimoto 16. Kymograph-based analysis of microtubule dynamics Ram Dixit 17. Simultaneous measurement of turgor pressure and cell wall elasticity in growing pollen tubes Ueli Grossniklaus 18. Methods for live cell imaging of pollen biology Leonor Boavida 19. Morphological analysis of Arabidopsis sepals Lilan Hong 20. Construction and use of a tensile device for onion epidermis Anja Geitmann 21. User-friendly assessment of pavement cell shape features with PaCeQuant: novel functions and tools Katharina Bürstenbinder 22. Imaging pavement cell development Arun Sampathkumar 23. Microfluidics systems for plant root imaging and analysis Guido Grossmann and Marjorie Guichard 24. Live imaging and morphometry of roots Ross Sozzani 25. Correlative electron and light microscopy of plant cells Jessica Marion

Cell biologists, biophysicists and physiologists who study plant cells and wish to expand their expertise to include new approaches

• Provide in-depth procedures and application notes from selected experts who developed the methods
• Each chapter will include figures and movies as appropriate to explain complex techniques
• Chapters will include caveats of techniques and future prospects