Pathogenic Yersinia, 1st ed. 2019 Methods and Protocols Methods in Molecular Biology Series, Vol. 2010

This volume delivers a compendium of detailed protocols to the research community in order to aid in the investigation and discovery of Yersinia virulence mechanisms using important in vivo and in vitro infection models, which have led to major advances in the field and in our understanding of Yersinia pathogen-host interactions. Beginning with a section on mouse models, the book continues with chapters covering the monitoring of bacteria during infection, invertebrate models, as well as Yersinia interaction with immune cells and immune signaling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

An intradermal model for Yersinia pestis inoculation.- Intranasal Inoculation of Mice with Yersinia pestis and Processing of Pulmonary Tissue for Analysis.- Standardized method for aerosol challenge of rodents with Yersinia pestis for modeling primary pneumonic plague.- Mouse Models of Yersiniosis.- Intravital Confocal Microscopy of Dermal Innate Immune Responses to Flea-transmitted Yersinia pestis.- Laser scanning microscopy of Yersinia pestis infected tissues.- Real-time monitoring of Yersinia pestis promoter activity by bioluminescence imaging.- Discovering Yersinia-Host Interactions by Tissue Dual RNA-seq.- Detection of cells translocated with Yersinia Yops in infected tissues using β-lactamase fusions.- Monitoring of neutrophil recruitment to mice lungs during pneumonic plague.- Co-infection assay to determine Yersinia pestis competitive fitness in fleas.- Drosophila as a model for understanding the insect host of Yersinia pestis.- Intracellular assays to monitor survival and growth of Yersinia pestis within macrophages.- Human neutrophil isolation and degranulation responses to Yersinia pestis infection.- Measurement of Yersinia translocon pore formation in erythrocytes.- Analysis of inflammasome activation in response to Yersinia infection by fluorescence microscopy detection of active caspase-1 puncta.- Methods for detection of pyrin inflammasome assembly in macrophages infected with Yersinia spp.

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts