Neisseria meningitidis, 1st ed. 2019 Methods and Protocols Methods in Molecular Biology Series, Vol. 1969

An Overview of Neisseria meningitides.- Diagnosis of Meningococcal Infection Using Internally-Controlled Multiplex Real-Time PCR.- Investigating RNA-Protein Interactions in Neisseria meningitidis by RIP-Seq Analysis.- Genome Sequencing and Interrogation of Genome Databases: A Guide to Neisseria meningitidis Genomics.- Determination of Repeat Number and Expression States of Phase-Variable Loci through Next Generation Sequencing and Bioinformatic Analysis.- Screening DNA Repeat Tracts of Phase Variable Genes by Fragment Analysis.- Sample Preparation for Proteomic Analysis of Neisseria meningitides.- Investigation of Whole Cell Meningococcal Glycan Interactions Using High Throughput Glycobiology Techniques: Glycan Array and Surface Plasmon Resonance.- The Detroit 562 Pharyngeal Immortalized Cell Line Model for the Assessment of Infectivity of Pathogenic Neisseria sp..- In VitroModels for Studying the Interaction of Neisseria meningitidis with Human Brain Endothelial Cells.- Modeling Neisseria meningitidis Infection in Mice: Methods and Logistical Considerations for Nasal Colonization and Invasive Disease.- Meningococcal Serogroup A, B, C, W, X, and Y Serum Bactericidal Antibody Assays.- Monoclonal Antibodies against the Capsular Polysaccharides A, C, Y, W, and X of Neisseria meningitides: A Platform for the Quality Control of Meningococcal Vaccines.- Meningococcal Antigen Typing System (MATS): A Tool to Estimate Global Coverage for 4CMenB, a Multicomponent Meningococcal B Vaccine.- Flow Cytometric Assays to Quantify fHbp Expression and Detect Serotype Specific Capsular Polysaccharides on Neisseria meningitides.- Assessment of fHbp Expression Level by Reverse Transcriptase Quantitative PCR and Promoter Sequence Analysis.