Neisseria gonorrhoeae, 1st ed. 2019 Methods and Protocols Methods in Molecular Biology Series, Vol. 1997

General topic area

Chapter title

1

Introduction – biology, disease, pathogenesis

2

Infection and AMR

Algorithms for predicting infection in women

3

Antimicrobial resistance

4

Whole-genome sequencing to predict antibiotic MICs for Ngo

5

?Developing new antimicrobials to gonococci (computer-based? Etc…)

6

Genetic transformation of Neisseria gonorrhoeae

7

Transcriptomics for Gc infection

8

RNA-seq for Neisseria gonorrhoeae

9

Biochemistry

Isolation of phenotypic variants by stereo-microscopy

10

Production of peptidoglycan

11

Protein glycosylation in Neisseria gonorrhoeae

12

?Production of purified pili/LPS

13

Vaccine

OMV preparation and characterization

14

Proteomics for vaccine development

15

16

VLP-based vaccines

17

Recombinant protein expression for gonococcal antigens

18

Biological assays

Serum bactericidal assay

19

Mouse intravaginal infection model

Human

21

Mouse CEACAM model of infection humanized mouse model

22

Gonococcal models of eye infection

Fallopian tube explant model

24

Neutrophil interactions with Neisseria gonorrhoeae

25

3D endometrial epithelial cell model

26

Rhesus macaque model of Neisseria infection

27

Methods to investigate gonococcal adhesion and invasion of human cells

28

Opsonophagocytosis of N.gonorrhoeae

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts