Lymphangiogenesis, 1st ed. 2018 Methods and Protocols Methods in Molecular Biology Series, Vol. 1846

Three-Dimensional Visualization of the Lymphatic Vasculature.- The Histological and Morphological Characterization of Developing Dermal Lymphatic Vessels.- Genetic Lineage Tracing of Lymphatic Endothelial Cells in Mice.- Visualization and Tools for Analysis of Zebrafish Lymphatic Development.- Characterization of Zebrafish Facial Lymphatics.- Correlative Fluorescence and Scanning Electron Microscopy to Study Lymphovenous Valve Development.- Characterization of Mouse Mesenteric Lymphatic Valve Structure and Function.- Morphological Analysis of Lacteal Structure in the Small Intestine of Adult Mice.- Morphological and Functional Analysis of CNS Associated Lymphatics.- Morphological Analysis of Schlemm's Canal in Mice.- Imaging Lymphatics in Mouse Lungs.- Imaging of Endothelial Cell Dynamic Behavior in Zebrafish.- Visualization and Measurement of Lymphatic Function In Vivo.- Investigating Effects of Fluid Shear Stress on Lymphatic Endothelial Cells.- Methods for Assessing the Contractile Function of Mouse Lymphatic Vessels Ex Vivo.- Evaluation and Characterization of Endothelial Cell Invasion and Sprouting Behavior.- Dorsal Ear Skin Window for Intravital Imaging and Functional Analysis of Lymphangiogenesis.- Isolation of Human Skin Lymphatic Endothelial Cells and 3D Reconstruction of the Lymphatic Vasculature In Vitro.- Stimulation and Inhibition of Lymphangiogenesis via Adeno-Associated Viral Gene Delivery.- Isolation and Characterization of Mouse Organ Specific Endothelial Transcriptomes.- Single Cell mRNA Sequencing of the Mouse Brain Vasculature.- Metabolic Analysis of Lymphatic Endothelial Cells.- Characterizing Epigenetic Changes in Endothelial Cells.