Kupffer Cells, 1st ed. 2020 Methods and Protocols Methods in Molecular Biology Series, Vol. 2164

This volume provides a complete overview of kupffer cell biology, from isolation to phenotyping. Chapters guide readers through methods and protocols on phenotyping, including RNA sequencing, Mass cytometry and 3D microscopy, as well as gene manipulation in vivo, and disease models from fatty liver disease to cancer.?Written in the highly successful?Methods in Molecular Biology?series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

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Authoritative and cutting-edge,?Kupffer Cells: Methods and Protocols? aims to ensure successful investigation of this growing field. 

1. Kupffer Cell and Hepatocytes Isolation by Gradient Centrifugation from a Single Mouse Liver

2.  Kupffer Cell Isolation from Human Biopsies

Cecilia Morgantini

3. Liver Macrophage Isolation by Flow Cytometry Sorting

André Sulen

4. Kupffer Cell Protein Isolation and Detection by Western Blot

Valerio Azzimato

5. Kupffer Cell mRNA Sequencing

Connie Xuand Emelie Barreby

6. Kupffer Cell and Monocyte-derived Macrophage Identification by Immunofluorescence on FFPE Mouse Liver Sections

Adrien Guillot, Chloé Buch, and Tony Jourdan

7. Sample Preparation of Optically Cleared Liver Tissue to Identify Liver Macrophages using 3D Microscopy

Otto Strauss and Niklas K. Björkström

8. Glucan-Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Kupffer cells in mouse liver

Michaela Tencerova

9. Engraftment of Human Hepatocytes in the PiZ-NSG Mouse Model

Qiushi Tang, Gwladys Gernoux, Yangfun Cheng, Terence Flotte, and Christian Mueller

10. Kupffer Cell Characterization by Mass Cytometry

Camille Blériot, Shamin Li, Muhammad Faris Bin Mohd Kairi, Evan Newell, and Florent Ginhoux

11. Enzymatic Quantification of Liver Lipids after Folch Extraction

Mirko E. Minniti, Osman Ahmed, and Matteo Pedrelli

12. In vivo Investigation of High Fat Diet Induced Hepatic Lipid Dysfunctions

Marion Korach-André

13. An Obesogenic Dietary Mouse Model of Nonalcoholic Steatohepatitis

Bader Zarroukiand Jeremie Boucher

14. Generating Hepatocellular Carcinoma in Mice By Expressing N-Ras^G12V Accompanied with Trp53 Silence Using A Transposon-based System Delivered via Hydrodynamic Ail Vein Injection

Sijia Yuand Santiago Vernia

15. Mouse Model of Alcoholic Steatohepatitis

Adeline Bertola

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts