Organ Regeneration, 1st ed. 2017 3D Stem Cell Culture & Manipulation Methods in Molecular Biology Series, Vol. 1597

This volume provides readers with a better understanding of organogenesis in developmental biology and next-generation organ regenerative therapy. This book focuses on recent studies of organ regeneration from stem cells using in vitro 3D cell culture and manipulation. The chapters cover topics such as generation of a 3D retinal tissue formation; functional salivary gland regeneration; liver regeneration using cultures liver bud; and in vivo model of small intestine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and thorough, Organ Regeneration: 3D Stem Cell Culture and Manipulation is a valuable resource for scientists and researchers who are interested in this field.

Dedication…
Preface…
Table of Contents…
Contributing Authors…

1       Generation of Various Telencephalic Regions from Human Embryonic Stem Cells in Three-Dimensional Culture
Taisuke Kadoshima, Hideya Sakaguch, and Mototsugu Eiraku

2       Generation of a Three-Dimensional Retinal Tissue from Self-Organizing Human ESC Culture
Atsushi Kuwahara, Tokushige Nakano, and Mototsugu Eiraku

3       3D Culture for Self-Formation of the Cerebellum from Human Pluripotent Stem Cells through Induction of the Isthmic Organizer
Keiko Muguruma

4       Reconstitution of a Patterned Neural Tube from Single Mouse Embryonic Stem Cells
Keisuke Ishihara, Adrian Ranga, Matthias P. Lutolf, Elly M. Tanaka, and Andrea Meinhardt

5        Functional Pituitary Tissue Formation
Chikafumi Ozone and Hidetaka Suga

6       Directed Differentiation of Mouse Embryonic Stem Cells into Inner Ear Sensory Epithelia in 3D Culture
Jing Nie, Karl R. Koehler, and Eri Hashino

7        Generation of Functional Thyroid Tissue using 3D-Based Culture of Embryonic Stem Cells
Francesco Antonica, Dominika Figini Kasprzyk, Andrea Alex Schiavo, Miriàn Romitti, and Sabine Costagliola

8        Functional Tooth Regeneration
Masamitsu Oshima, Miho Ogawa, and Takashi Tsuji 

9        Functional Hair Follicle Regeneration by the Rearrangement of Stem Cells
Kyosuke Asakawa, Koh-ei Toyoshima, and Takashi Tsuji

10     Functional Salivary Gland Regeneration
Miho Ogawa and Takashi Tsuji

11     Generation of a Bioengineered Lacrimal Gland by using the Organ Germ Method
Masatoshi Hirayama and Takashi Tsuji

12     Generation of Gastrointestinal Organoids from Human Pluripotent Stem Cells
Jorge O. Múnera and James M. Wells

13     Generation of a Three-Dimensional Kidney Structure from Pluripotent Stem Cells
Yasuhiro Yoshimura, Atsuhiro Taguchi, and Ryuichi Nishinakamura

14     Making a Kidney Organoid using the Directed Differentiation of Human Pluripotent Stem Cells
Minoru Takasato and Melissa H. Little

15     Liver Regeneration using Cultured Liver Bud
Keisuke Sekine, Takanori Takebe, and Hideki Taniguchi

16     Formation of Stomach Tissue by Organoid Culture using Mouse Embryonic Stem Cells
Taka-aki K. Noguchi and Akira Kurisaki

17     In Vivo Model of Small Intestine
Mahe M. Maxime, Nicole E. Brown, Holly M. Poling, and Helmrath A. Michael 

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras