Hepatic Stellate Cells, 2023 Methods and Protocols Methods in Molecular Biology Series, Vol. 2669

This detailed volume explores hepatic stellate cells, which have become a spotlight of liver cell biology due to their pleiotropic functions that extend well beyond extracellular matrix production to pivotal roles in hepatic homeostasis, immunity, and metabolism. The book features methods to isolate, evaluate, and manipulate this cell type in an effort to elucidate hepatic biology and establish prospects for treating disease. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. 

Isolation, Purification, and Culture of Primary Murine Hepatic Stellate Cells: An Update.- Differentiation of Hepatic Stellate Cells from Pluripotent Stem Cells.- Testing Cell Migration, Invasion, Proliferation, and Apoptosis in Hepatic Stellate Cells.- Phalloidin Staining for F-Actin in Hepatic Stellate Cells.- Retinyl Ester Analysis by Orbitrap Mass Spectrometry.- Studying Hepatic Stellate Cell Senescence.- Isolation of Hepatic Stellate Cells and Lymphocytes for Co-Culture Systems.- Working with Immortalized Hepatic Stellate Cell Lines.- Induction of Obstructive Cholestasis in Mice.- Mouse Models for Hepatic Stellate Cell Activation and Liver Fibrosis Initiation.- Generation and Culture of Primary Mouse Hepatocyte-HSC Spheroids.- Hepatic Stellate Cell Depletion and Genetic Manipulation.- Human Hepatic Stellate Cells: Isolation and Characterization.- Decellularization of Human Liver to Generate Native Extracellular Matrix for Use in Automated Functional Assays with Stellate Cells.- Multiplex Immunostaining to Spatially Resolve the Cellular Landscape in Human and Mouse Livers.- Single Cell Secretome Analyses of Hepatic Stellate Cells: Aiming for Single Cell Phenomics.- Hepatic Stellate Cells Targeting Using Peptide-Modified Biologicals.- Experimental Workflow for Preclinical Studies of Human Antifibrotic Therapies.

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts