Embryonic Stem Cell Protocols (4^th Ed., 4th ed. 2022) Methods in Molecular Biology Series, Vol. 2520

1. Dissecting Molecular Phenotypes through FACS-Based Pooled CRISPR Screens

            Oriana Genolet, Liat Ravid Lustig, and Edda G. Schulz

2. Feeder Free Human Embryonic Stem Cell Culture under Defined Culture Conditions

            Taha Bartu Hayal and Ayşegül Doğan

3. Rat Induced Pluripotent Stem Cells-Derived Cardiac Myocytes in a Cell Culture Dish

            Fatima Bianca A. Dessouki, Pawan K. Singal, and Dinender K. Singla

4. Transient Induction and Characterization of Mouse Epiblast-Like Cells from Mouse Embryonic Stem Cells

            Federico Pecori and Shoko Nishihara

5. High-Content Image Analysis of Spatiotemporal Proliferation and Differentiation Patterns in 3D Embryoid Body Differentiation Model

            Olga Gordeeva

6. Derivation of Multipotent Neural Progenitors from Human Embryonic Stem Cells for Cell Therapy and Biomedical Applications

            Loriana Vitillo and Ludovic Vallier

7. Feeder Dependent/Independent Mouse Embryonic Stem Cell Culture Protocol

            Hatice Burcu Şişli, Selinay Şenkal, Derya Sağraç, Taha Bartu Hayal, and Ayşegül Doğan

8. ChIP-qPCR for Polycomb Group Proteins during Neuronal Differentiation of Human Pluripotent Stem Cells

            Divya Desai and Prasad Pethe

9. Directed Differentiation of Human Pluripotent Stem Cells into Inner Ear Organoids

            Yoshitomo Ueda, Stephen T. Moore, and Eri Hashino

10. Glycolytic Profiling of Mouse Embryonic Stem Cells (mESCs)

            Bibiana Correia, Maria Inês Sousa, and João Ramalho-Santos

11. Replating Protocol for Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

            Arzuhan Koc and Esra Cagavi

12. Hematopoietic Cell Isolation by Antibody-Free Flow Cytometry in the Zebrafish Embryo

            Katsuhiro Konno, Jingjing Kobayashi-Sun, Fumio Arai, Isao Kobayashi, and Daisuke Sugiyama

13. Human Trophectoderm Spheroid Derived from Human Embryonic Stem Cells

            Wen Huang, Sze Wan Fong, William Shu Biu Yeung, and Yin-Lau Lee

14. Embryoid Bodies-Based Multilineage Differentiation of Human Embryonic Stem Cells Grown on Feeder-Free Conditions

            Luciana Isaja, Sofía Luján Ferriol-Laffouillere, Sofía Mucci, María Soledad Rodríguez-Varela, and Leonardo Romorini

15. A Simple, Rapid, and Cost-effective Method for Loss-of-Function Assays in Pluripotent Cells

            Helena Covelo-Molares, Yara Souto, Miguel Fidalgo, and Diana Guallar

16. Embryoid Body Formation from Mouse and Human Pluripotent Stem Cells for Transplantation to Study Brain Microenvironment and Cellular Differentiation

            Magdalena Guerra-Crespo, Omar Collazo-Navarrete, Rodrigo Ramos-Acevedo, Carmen Alejandra Morato-Torres, and Birgitt Schüle

17. A Modified SMART-Seq Method for Single Cell Transcriptomic Analysis of Embryoid Body Differentiation

            Jianqun Zheng, Ying Ye, Qiushi Xu, Wei Xu, Wensheng Zhang, and Xi Chen

18. Osteogenic Differentiation from Mouse Embryonic Stem Cells

            Zahra Alvandi and Michal Opas

19. Murine Embryonic Stem Cell Culture, Self-Renewal, and Differentiation

            Manar Elkenani and Belal A. Mohamed

20. Mouse Embryonic Stem Cell Culture in Serum-Containing or 2i Conditions

            Emre Balbasi, Gozde Guven, and Nihal Terzi Çizmecioğlu

21. Directed Differentiation of Mouse Embryonic Stem Cells to Mesoderm, Endoderm, and Neuroectoderm Lineages

            Emre Balbasi, Dersu Sezginmert, Ceren Alganatay, and Nihal Terzi Çizmecioğlu

22. Accessing the Human Pluripotent Stem Cell Translatome by Polysome Profiling

            Rubens Gomes-Júnior, Patrícia Shigunov, Bruno Dallagiovanna, and Isabela Tiemy Pereira

23. Genome Engineering Human ESCs or iPSCs with Cytosine and Adenine Base Editors

            Giulia Pavani, Joshua Klein, Deborah L. French, and Paul Gadue

24. Proteomic Analysis of Human Neural Stem Cells Differentiation by SWATH-MS

            Jirina Tyleckova, Jakub Cervenka, Ievgeniia Poliakh, Jaromir Novak, Katerina Vodickova Kepkova, Helena Kupcova Skalnikova, and Petr Vodicka

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts