Cilia, Softcover reprint of the original 1st ed. 2016 Methods and Protocols Methods in Molecular Biology Series, Vol. 1454

This volume is the result of an explosion of molecular-based research on Cilia, which began with the discovery of the universality of intraflagellar transport (IFT) and ciliary genomics/proteomics. The chapters in this book cover topics such as: high resolution imaging and functional characterization of sensory and primary cilia in mammalian cells and zebrafish, methods to study ciliary-mediated chemoresponse in Paramecium, and methods to study centrosomes and cilia in C. elegans and Drosophila. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and cutting-edge, Cilia: Methods and Protocols is broad and covers motile, sensory, and primary cells. It is a valuable resource to anyone interested in entering the field of ciliary biology using model organisms, including flagellate algae, ciliates, planaria, nematodes, insects, zebrafish, and mammalian cells.

1. Methods for Studying Ciliary Import Mechanisms
Daisuke Takao and Kristen J. Verhey

2. Targeting of ASH Domain-Containing Proteins to the Centrosome
Patricia Verdier, Stine K. Morthorst, and Lotte B. Pedersen

3. Morphological and Functional Characterization of the Ciliary Pocket by Electron and Fluorescence Microscopy
Rania Ghossoub, Louise Lindbæk, Anahi Molla-Herman, Alain Schmitt, Søren Tvorup Christensen, and Alexandre Benmerah

4. Methods to Study Interactions between Ciliogenesis and Autophagy
Birgit H. Satir and Olatz Pampliega

5. Recombinant Reconstitution and Purification of the IFT B Core Complex from Chlamydomonas reinhardtii
Michael Taschner and Esben Lorentzen

6. Methods for Studying Movement of Molecules within Cilia
Karl F. Lechtreck

7. A FRAP-Based Method for Monitoring Molecular Transport in Ciliary Photoreceptor Cells In Vivo
Kirsten A. Wunderlich and Uwe Wolfrum

8. Kymographic  Analysis of Transport in an Individual Neuronal Sensory Cilium in Caenorhabditis elegans
Robert O’Hagan and Maureen M. Barr

9. Visualization and Manipulation of Cilia and Intraciliary Calcium in the Zebrafish Left-Right Organizer
Shiaulou Yuan and Martina Brueckner

10. Methods for Studying Ciliary-Mediated Chemoresponse in Paramecium
M.S. Valentine and J.L. Van Houten

11. STED and STORM Superresolution Imaging of Primary Cilia
T. Tony Yang, Weng Man Chong, and Jung-Chi Liao

12. CLEM Methods for Studying Primary Cilia
Frank P. Macaluso, Geoffrey S. Perumal, Johan Kolstrup, and Peter Satir

13. Methods for Visualization of Neuronal Cilia
Tamara Caspary, Daniela Marazziti, and Nicolas F. Berbari

14. Methods to Study Centrosomes and Cilia in Drosophila
Swadhin Chandra Jana, Susana Mendonça, Sascha Werner, and Monica Betterncourt-Dias

15. Analysis of Axonemal Assembly during Ciliary Regeneration in Chlamydomonas
Emily L. Hunter, Winfield S. Sale, and Lea M. Alford

16. Planaria as a Model System for the Analysis of Ciliary Assembly and Motility
Stephen M. King and Ramila S. Patel-King

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts