Beta-Arrestins, 1st ed. 2019 Methods and Protocols Methods in Molecular Biology Series, Vol. 1957
Coordonnateurs : Scott Mark G. H., Laporte Stéphane A.
This volume looks at various approaches to study the pleiotropic roles of b-arrestins (b-arrs) in the control of signal transduction, and the resulting cellular and in vivo consequences that arise. The chapters in this book cover diverse topics around b-arrs such as their established roles in GPCR regulation and trafficking; regulatory scaffolding functions of b-arrs in MAPK signaling, cAMP hydrolysis and cytoskeletal dynamics; proteomic analysis of the b-arr interactome; mathematical modelling of b-arr signaling networks; functional selectivity involving biased ligands; nucleocytoplasmic trafficking and primary cilia-associated functions of b-arrs; conformational plasticity of b-arrs; and the roles of b-arrs in allergic inflammation, Type 2 Diabetes, Parkinson?s disease, and cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Beta-Arrestins: Methods and Protocols is a valuable resource for researchers interested in learning more about the function and regulation of b-arrestins.
Preface…
Table of Contents…
Contributing Authors…
Part I Introduction
1. A Brief History of the b-Arrestins
Marc G. Caron and Lawrence S. Barak
2. b-Arrestins: Multitask Scaffolds Orchestrating the Where and When In Cell Signaling
Stéphane A. Laporte and Mark G.H. Scott
Part II Methods to Study Roles of b-Arrestins in GPCR Regulation
3. Methods to Monitor the Trafficking of β-Arrestin/G Protein-Coupled Receptor Complexes using Enhanced Bystander BRET
Yubo Cao, Yoon Namkung, and Stéphane A. Laporte
4. Methods to Investigate b-Arrestin-Mediated Regulation of GPCR Function in Human Airway Smooth Muscle
Tonio Pera and Raymond B. Penn
5. Measuring Recruitment of b-Arrestin to G Protein-Coupled Heterodimers using Bioluminescence Resonance Energy Transfer
Dany Fillion, Dominic Devost, and Terence E. Hébert
6. Detection of β-Arrestin-Mediated G Protein-Coupled Receptor Ubiquitination using BRET
Karim Nagiand Sudha K. Shenoy
Part III Methods to Study Scaffolding Functions of b-Arrestins
7. Using In Vitro Pull-Down and In Cell Overexpression Assays to Study Protein Interactions with Arrestin
8. Methods to Investigate Arrestins in Complex with Phosphodiesterases
Amy Tibbo, Tara Busiau, and George S. Baillie
9. Methods to Characterize Protein Interactions with β-arrestin in cellulo
Revu Ann Alexander, Isaure Lot, and Hervé Enslen
10. Methods to Investigate the β-Arrestin-Mediated control of ARF6 Activation to Regulate Trafficking and Actin Cytoskeleton Remodeling
Ricardo Charles, Mohamed Bourmoum, Shirley Campbell, and Audrey Claing
11. Methods to Investigate the Roles of b-Arrestin-Dependent RalGDS Activation in GPCR-Stimulated Membrane Blebbing
Stephen S. G. Ferguson
12. Methods to Determine Interaction Interfaces between b-Arrestins and Their Protein Partners
Thomas Bourquard, Astrid Musnier, Aurélie Tréfier, Flavie Landomiel, Thomas Boulo, Eric Reiter, Pascale Crépieux, and Anne Poupon
13. Workflow Description to Dynamically Model β-Arrestin Signaling Networks
14. Proteomic Analysis of the β-Arrestin Interactome
Yang Zhao and Kunhong Xiao
Part IV Methods to Analyse Ligand Bias
15. Quantitating Ligand Bias using the Competitive Model of Ligand Activity
Edward L. Stahl, Frederick J. Ehlert, and Laura M. Bohn
Part V Methods to Study b-Arrestin Localization
16. Methods to Investigate the Nucleocytoplasmic Shuttling Properties of b-Arrestins
Elodie Blondel-Tepaz, Thomas Guilbert, and Mark G.H. Scott
17. Monitoring β-Arrestin2 Targeting to the Centrosome, Basal Body and Primary Cilium by Fluorescence Microscopy
Anahi Molla-Herman, Kathryn M. Davis, Kirk Mykytyn, and Alexandre Benmerah
Part VI Methods to Study Conformational Change of b-Arrestin
18. A Mass Spectrometry-Based Structural Assay for Activation-Dependent Conformational Changes in β-Arrestins
Yang Zhao and Kunhong Xiao
19. Probing Arrestin Function using Intramolecular FlAsH-BRET Biosensors
Erik G. Strungs, Louis M. Luttrell, and Mi-Hye Lee
Part VII Methods to Study Roles of b-Arrestins in Physiopathology
20. Methods to Study the Roles of b-Arrestins in Meningococcal Signaling
Zoé Virion, Stefano Marullo, and Mathieu Coureuil
21. Methods to Investigate the Roles for β-Arrestin2 in Allergic Inflammatory Airway Disease
Akhil Hegde and Julia K. L. Walker
22. Methods to Study Roles of β-Arrestins in the Regulation of Pancreatic β-Cell Function
Stéphane Dalle, Safia Costes, Gyslaine Bertrand, and Magalie A. Ravier
23. Methods to Investigate β-Arrestin Function in Metabolic Regulation
Bing Luan, Jian Zhao, and Gang Pei
24. Methods to Investigate the Role of b-Arrestin Signaling in Parkinson's Disease
Nikhil M. Urs
25. Methods to Investigate β-Arrestin-1/β-Catenin Signaling in Ovarian Cancer Cells
Laura Rosanò, Roberta Cianfrocca, and Anna Bagnato
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Date de parution : 03-2019
Ouvrage de 409 p.
17.8x25.4 cm
Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).
Prix indicatif 158,24 €
Ajouter au panierThèmes de Beta-Arrestins :
Mots-clés :
cell signalling; GPCR; cAMP; cell migration; cell adhesion; Receptors
