Bacteriophages, Softcover reprint of the original 1st ed. 2018 Methods and Protocols, Volume 3 Methods in Molecular Biology Series, Vol. 1681
This book expands on the previous volumes with new chapters exploring emerging themes and methodologies in bacterial virus research. The chapters in this book are divided into 4 parts and cover topics such as: iron chloride flocculation of bacteriophages from seawater; encapsulation of Listeria phage A511 by alginate; examining genome termini of bacteriophage through high-throughput sequencing; genome sequencing of dsDNA-containing bacteriophages directly from a single plaque; characterizing bacteriophages by biology, taxonomy, and genome analysis; phage genome annotation using the RAST pipeline; and the use of RP4::mini-Mu for gene transfer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and authoritative, Bacteriophages: Methods and Protocols, Volume III is a valuable resource for both established and novice phage scientists.
Table of Contents…
Contributing Authors…
Part I Key Basics of Phage Biology
1. Basic Phage Mathematics
Stephen T. Abedon and Tena I. Katsaounis
2. Analysis of Host-Takeover during SPOi Infection of Bacillus subtilis
Charles Stewart
3. Practical Advice on the One-Step Growth Curve
Andrew M. Kropinski
4. Iron Chloride Flocculation of Bacteriophages from Seawater
Bonnie T. Poulos, Seth G. John, and Matthew B. Sullivan
5. Purification of Bacteriophages using Anion-Exchange Chromatography
Dieter Vandenheuvel, Sofie Rombouts, and Evelien M. Adriaenssens
6. Encapsulation Strategies of Bacteriophages (Felix O1) for Oral Therapeutic Application
Golam S. Islam, Qi Wang, and Parviz M. Sabour
7. Encapsulation of Listeria Phage A511 by Alginate to Improve its Thermal Stability
Hanie Ahmadi, Qi Wang, Loong-Tak Lim, and Sampathkumar Balamurugan
8. Application of a Virucidal Agent to Avoid Over-Estimation of Phage Kill during Phage Decontamination Assays on Ready-to-Eat Meats
Andrew Chibeu and S. Balamurugan
Part II Sequencing Analysis of Bacteriophages
9. Sequencing, Assembling, and Finishing Complete Bacteriophage Genomes
Daniel A. Russell
10. Identification of DNA Base Modifications by Means of Pacific Biosciences RS Sequencing Technology
Philip Kelleher, James Murphy, Jennifer Mahony, and Douwe van Sinderen
11. Analyzing Genome Termini of Bacteriophage Through High-Throughput Sequencing
Xianglilan Zhang, Yahui Wang, and Yigang Tong
12. Amplification for Whole Genome Sequencing of Bacteriophages from Single Isolated Plaques using SISPA
Derick E. Fouts
13. Genome Sequencing of dsDNA-Containing Bacteriophages Directly from a Single Plaque
Witold Kot
14. Preparing cDNA Libraries from Lytic Phage Infected Cells for Whole Transcriptome Analysis by RNA-Seq
Bob Blasdel, Pieter-Jan Ceyssens, and Rob Lavigne
Part III Phage-Related Bioinformatics Tools
15. Essential Steps in Characterizing Bacteriophages: Biology, Taxonomy, and Genome Analysis
16. Annotation of Bacteriophage Genome Sequences using DNA Master: An Overview
Welkin H. Pope and Deborah Jacobs-Sera
17. Phage Genome Annotation using the RAST Pipeline
Katelyn McNair, Ramy K. Aziz, Gordon D. Pusch, Ross Overbeek, Bas E. Dutilh, and Robert Edwards
18. Visualization of Phage Genomic Data: Comparative Genomics and Publication-Quality Diagrams
Dann Turner and J. Mark Sutton, Darren M. Reynolds, Eby M. Sim, and Nicola K. Petty
Part IV Bacteriophage Genetics
19. Transposable Bacteriophages as Genetic Tools
20. Applications of the Bacteriophage Mu In Vitro Transposition Reaction and Genome Manipulation via Electroporation of DNA Transposition Complexes
21. Use of RP4::mini-Mu for Gene Transfer
22. Muprints and Whole Genome Insertion Scans: Methods for Investigating Chromosome
Accessibility and DNA Dynamics using Bacteriophage Mu
N. Patrick Higgins
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Date de parution : 08-2018
Ouvrage de 316 p.
17.7x25.4 cm
Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).
Prix indicatif 84,39 €
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17.7x25.4 cm