Bacteriophages, 1st ed. 2019 Methods and Protocols, Volume IV Methods in Molecular Biology Series, Vol. 1898

This book expands on the previous volumes with new chapters focusing on functional characterization of phage and their proteins, and on the development of phage therapy by outlining novel models. The chapters in this book cover molecular topics such as PhageFISH for monitoring phage infections at single cell level; the analysis of phage-host protein-protein interactions using Strep-tag® II purifications; and also application driven chapters including 'duckweed (Lemna minor) and alfalfa (Medicago sativa) as bacterial infection model systems?. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Innovative and thorough, Bacteriophages: Methods and Protocols, Volume IV is a valuable resource for both established and novice phage scientists.

Preface…

Table of Contents…

Contributing Authors…

1. PhageFISH for Monitoring Phage Infections at Single Cell Level

Cristina Moraru and Jimena Barrero-Canosa

2. Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria

Mariana Piuri and Grahan Hatfull

3. Engineering Bacteriophage-Based Biosensors

Daniel Brownell, John King, Brian Caliando, Lada Sycheva, and Michael Koeris

4. Introduction of Phage Genome into Escherichia coli by Electroporation

Nika Janež, Saša Haberl Meglič, Karel Flisar, Damijan Miklavčič, and Matjaž Peterka

5. Site-Specific Mutagenesis of Bacillus subtilis Phage SPO1

Charles R. Stewart

6. Genetic Manipulation of Lytic Bacteriophages with BRED: Bacteriophage Recombineering of Electroporated DNA

Laura J. Marinelli, Mariana Piuri, and Graham F. Hatfull

7. Isolation of Competitive Phage Display-Modified Bacteriophage T4 with Affinity Chromatography

Krystyna Dąbrowska

8. Immobilization of Intact Phage and Phage-Derived Proteins for Detection and Biocontrol Purposes

Hany Anany, Luba Brovko, Denis Arutyunov, Nilufar Poshtiban, Amit Singh, Upasana Singh, Michael Brook, Christine Szymanski, Stephane Evoy, and Mansel Griffiths

9. Peptidoglycan Hydrolytic Activity of Bacteriophage Lytic Proteins in Zymogram Analysis

Lorena Rodríguez-Rubio, David M. Donovan, Beatriz Martínez, Ana Rodríguez, and Pilar García

10. Analyzing Phage-Host Protein-Protein Interactions using Strep-tag® II Purifications

Jeroen De Smet, Hanne Hendrix, and An Van den Bossche

11. Techniques to Assess Phage/Biofilm Interaction

Diana Vilas Boas, Carina Almeida, Nuno Azevedo, Sanna Sillankorva, and Joana Azeredo

12. Screening for Growth-Inhibitory ORFans in Pseudomonas aeruginosa-Infecting Bacteriophages

Hanne Hendrix, Ines Staes, Abram Aertsen, and Jeroen Wagemans

13. The Use of the greater Wax Moth Larvae (Galleria mellonella) as an Alternative Animal Infection Model for the Analysis of Bacterial Pathogenesis

Fatima Kamal, Danielle L. Peters, Jaclyn G. McCutcheon, Gary B. Dunphy, and Jonathan J. Dennis

14. Use of a Silkworm Larva Model in Phage Therapy Experiments

Jumpei Uchiyama, Iyo Takemura-Uchiyama, and Shigenobu Matsuzaki

15. Exploitation of Drosophila Infection models to Evaluate Antibacterial Efficacy of Phages

Hye-Jeong Jang, Hee-Won Bae, and You-Hee Cho

16. Duckweed (Lemna minor) and Alfalfa (Medicago sativa) as Bacterial Infection Model Systems

Fatima Kamal, Alina Radziwon, Carly M. Davis, and Jonathan J. Dennis

17. Quantitating Phage Efficacy in ready-To-Eat Meats

Andrew Chibeu and Sampathkumar Balamurugan

18. Use of a Chicken Embryo Lethality Assay to Assess the Efficacy of Phage Therapy

Angélina Trotereau and Catherine Schouler

Index…

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts