Electrophoretic Separation of Proteins, 1st ed. 2019 Methods and Protocols Methods in Molecular Biology Series, Vol. 1855
Preface…
Table of Contents…
Contributing Authors…
1. How It All Began: A Personal History of Gel-Electrophoresis
Oliver Smithies
2. Introduction to Protein Electrophoresis
Pothur R. Srinivas
3. Concentrating Proteins by Salt, Polyethylene Glycol, Solvent, SDS Precipitation, Three Phase Partitioning, Dialysis, Centrifugation, Ultrafiltration, Lyophilisation, Affinity Chromatography, Immunoprecipitation or Increased Temperature for Protein Isolation, Drug Interaction, and Proteomic and Peptidomic Evaluation
J.P. Dean Goldring
4. Lysis Buffer Choices are Key Considerations to Ensure Effective Sample Solubilization for Protein Electrophoresis
Ewa I. Miskiewicz and Daniel J. MacPhee
5. The Cydex Blue Assay: A One Step Protein Assay for Samples Prior to SDS Electrophoresis
Thierry Rabilloud
6. Cellulose-Acetate Electrophoresis of Hemoglobin
Ramesh Kumar and Wilbert A. Derbigny
7. Native Polyacrylamide gels
Claudia Arndt, Stefanie Koristka, Anja Feldmann, and Michael Bachmann
8. Isoelectric Focusing on Non-Denaturing Gels
Biji T. Kurien and R. Hal Scofield
9. Determination of protein Molecular weights on SDS-PAGE
Hiroyuki Matsumoto, Hisao Haniu, and Naoka Komori
10. Two-Dimensional Gel Electrophoresis by Glass Tube-Based IEF and SDS-PAGE
Hiroyuki Matsumoto, Hisao Haniu, Biji T. Kurien, and Maoka Komori
11. Cationic Electrophoresis
Engelbert Buxbaum
12. Two-Dimensional Gel Electrophoresis with Immobilized pH Gradients
Bre’Ana Byrd and Huyen Tran
13. SARCOSYL-PAGE: Optimized Protocols for the Separation and Immunological Detection of PEGylated Proteins
Christian Reichel, Günter Gmeiner, Philipp Reihlen, Mario Thevis, and Wilhelm Schänzer
14. Tricine-SDS-PAGE
Syed R. Haider, Helen J. Reid, and Barry L. Sharp
15. Analysis of Protein Glycation using Phenylboronate Acrylamide Gel Electrophoresis
Marta P. Pereira Morais, Omar Kassaar, Stephen E. Flower, Robert J. Williams, Tony D. James, and Jean M. H. Van den Elsen
16. Immunofixation Electrophoresis for Identification of Proteins and Specific Antibodies
Gyorgy Csako
17. Electrophoretic Separation of Very Large Molecular Weight Proteins in SDS-Agarose
Marion L. Greaser and Chad M. Warren
18. Increase in Local Protein Concentration by Field-Inversion Gel Electrophoresis
Henghang Tsai and Hon-chiu Eastwood Leung
19. Two-Dimensional Difference Gel Electrophoresis
Malachi Blundon, Vinitha Ganesan, Brendan Redler, Phu T. Van, and Jonathan S. Minden
20. Immunoelectrophoresis: A Method with Many Faces
Gyorgy Csako
21. Tris-Acetate Polyacrylamide Gradient Gels for the Simultaneous Electrophoretic Analysis of Proteins if Very High and Low Molecular Mass
Monica Cubillos-Rojas, Fabiola Amair-Pinedo, Irantzu Tato, Ramon Bartrons, Francesc Ventura, and Jose Luis Rosa
22. Diagnoal Electrophoresis for Detection of Proteins Involved in Disulfide Bonds
Ronald Saraswat and Brian McDonagh
23. Identification of Proteins on Archived 2D Gels
Hiroyuki Matsumoto, Nobuaki Takemori, and Naoka Komori
24. Two-Dimensional Gel Electrophoresis: Vertical Isoelectric Focusing
Yaser Dorri
25. A Foodomics Approach: CE-MS for Comparative Metabolomics of Colon Cancer Cells Treated with Dietary Polyphenols
Mustafa Çelebier, Clara Ibáñez, Carolina Simó, and Alejandro Cifuentes
26. Characterization of New Cyclic D, L – α – Alternate Amino Acid Peptides by Capillary Electrophoresis Coupled to Electrospray Ionization Mass Spectrometry
María Dámaris Cortez-Díaz, Fanny d’Orlyé, and Anne Varenne
27. “Microchip Electrophoresis”, with Respect to “Profiling of Aβ Peptides in the Cerebrospinal Fluid of Patients with Alzheier’s Disease”
Mohamad Reza Mohamadi, Romain Verpillot, Myriam Taverna, Markus Otto, and Jean-Louis Viovy
28. Measuring Low-Picomolar Apparent Binding Affinities by Minigel Electrophoretic Mobility Shift
Karen A. Lewis, Sarah E. Altschuler, and Deborah S. Wuttke
29. Identification of Proteins Interacting with Single Nucleotide Polymorphisms (SNPs) by DNA Pull-Down Assay
Bhupinder Singh and Swapan K. Nath
30. Horizontal Agarose Gel Mobility Shift Assay for Protein-RNA Complexes
Jennifer A. Ream, L. Kevin Lewis, and Karen A. Lewis
31. Applications of Immobilized Metal affinity Electrophoresis
Bao-Shiang Lee, Lasanthi P. Jayathilaka, Jin-Sheng Huang, and Shalini Gupta
32. Isoelectric Focusing in Agarose Gel for Detection of Oligoclonal Bands in Cerebrospinal and Other Biological Fluids
Gyorgy Csako
33. A Combined Free Flow Electrophoresis and DIGE Approach to Compare Proteins in Complex Biological Samples
Kim Y.C. Fung, Chris Cursaro, Tanya Lewanowitsch, Leah Cosgrove, and Peter Hoffmann
34. SDS PAGE for 35S Immunoprecipitation and Immunoprecipitation Western Blotting
Edward P. Trieu and Ira N. Targoff
35. A Multichannel Gel Electrophoresis and Continuous Fraction Collection Apparatus for High-Throughput Protein Separation and Characterization
Ming Dong, Megan Choi, Mark D. Biggin, and Jian Jin
36. Cell Surface Protein Biotinylation for SDS-PAGE Analysis
Giuliano Elia
37. Isolation of Proteins from Polyacrylamide Gels
Stefanie Koristka, Claudia Arndt, Ralf Bergmann, and Michael Bachmann
38. Continuous Elution SDS-PAGE with a Modified Standard Gel Apparatus to Separate and Isolate an Array of Proteins from Complex Mixtures
Robert G.E. Krause and J.P. Dean Goldring
39. Protein Extraction from Gels: A Brief Review
Biji T. Kurien, Rachna Aggarwal, and R. Hal Scofield
40. Gel Absorption-Based Sample Preparation Method for Shotgun Analysis of Membrane Proteome
Xianchun Wang and Songping Liang
41. Ultra-Rapid Sodium Dodecyl Sulfate Polyacrylamide Mini-Gel Electrophoresis
Rohit Thomas and Biji T. Kurien
James D. Fesmire
43. Single-Cell High Resolution Detection and Quantification of Protein Isoforms Differing by a Single Charge Unit
Kristi A. Koelsch
44. Artifacts and Common Errors in Protein Gel Electrophoresis
Biji T. Kurien and R. Hal ScofieldIncludes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Date de parution : 11-2018
Ouvrage de 523 p.
17.8x25.4 cm
Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).
Prix indicatif 242,64 €
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Mots-clés :
gel-electrophoresis; homogenization; Lysis Buffer; PAGE; PEGylated Proteins