X-Chromosome Inactivation, Softcover reprint of the original 1st ed. 2018 Methods and Protocols Methods in Molecular Biology Series, Vol. 1861

This book details recently developed technologies and conventionally employed cytological proceduresfor the study of X-Chromosome Inactivation. Chapters detail live imaging, bioinformatic methods, fluorescence in situ hybridization, and immunofluorescence, and procedures to optimize the study of molecular mechanism underlying X chromosome inactivation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, X-Chromosome Inactivation: Methods Protocols aims to be useful for researchers in the field of epigenetics, chromatin, noncoding RNA, and nuclear architecture.

1. Screening for Factors Involved in X Chromosome Inactivation using Haploid ESCs

Asun Monfort, Giulio Di Minin, and Anton Wutz

2. Unbiased Genetic Screen to Identify Factors Involved in X-chromosome Inactivation Using a Pooled Bar-coded shRNA Library

Benoit Moindrot and Neil Brockdorff

3. Unbiased Genetic Screen to Identify Factors Involved in X-chromosome Inactivation Using a Pooled Bar-coded shRNA Library

Benoit Moindrotand Neil Brockdorff

4. Manipulation of Xist Imprinting in Mouse Pre-implantation Embryos

Atsushi Fukuda, Akihiro Umezawa, and Hidenori Akutsu

5. Somatic Cell Nuclear Transfer in Mice — Basic Protocol and Its Modification for Correcting X Chromosome Inactivation Status

Kimiko Inoue, Shogo Matoba, and Atsuo Ogura

Osamu Masui, Edith Heard, and Haruhiko Koseki

7. Live Imaging of X-chromosome Inactivation and Reactivation Kinetics

Shin Kobayashi

8. Visualizing the Dynamics of Inactive X chromosomes in Living Cells Using Antibody-based Fluorescent Probes

Yuko Sato, Timothy J Stasevich, and Hiroshi Kimura

9. Visualization of Hidden Epitopes at the Inactive X Chromosome

Emma R Stewart and Dawn Coverley

Kris G. Alavattam, Hironori Abe, Akihiko Sakashita, and Satoshi H. Namekawa

11. Simultaneous RNA-DNA FISH in Mouse Pre-implantation Embryos

Aristea Magaraki, Agnese Loda, Joost Gribnau, and Willy M. Baarends

12. Combined Immunofluorescence, RNA FISH and DNA FISH in Pre-implantation Mouse Embryos

Ikuhiro Okamoto

13. RNA-FISH and Immunofluorescence of Mouse Pre- and Post-implantation Embryos

Hirosuke Shiura, Yuka Sakata, Kuniya Abe, and Takashi Sado

14. Experimental Analysis of Imprinted Mouse X-chromosome Inactivation

Marissa Cloutier, Clair Harris, Srimonta Gayen, Emily Maclary, and

Sundeep Kalantry

15. X-chromosome Inactivation and Escape from X inactivation in Mouse

Wenxiu Ma, Giancarlo Bonora, Joel B Berletch, Xinxian Deng, William S. Noble, and Christine M. Disteche

16. Practical Analysis of Hi-C Data: Generating A/B Compartment Profiles

Hisashi Miura, Rawin Poonperm, Saori Takahashi, and Ichiro Hiratani

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts