Capturing Chromosome Conformation, 1st ed. 2021 Methods and Protocols Methods in Molecular Biology Series, Vol. 2157

1. Capturing Chromosome Conformation

            Michel Pucéat

2. The Chromosome Conformation Capture (3C) in Drosophila melanogaster

            Federica Lo Sardo

3. 4C-Seq: Interrogating Chromatin Looping with Circular Chromosome Conformation Capture

            Nezih Karasu and Tom Sexton

4. Analysis, Modeling, and Visualization of Chromosome Conformation Capture Experiments

            Marco Di Stefano, David Castillo, François Serra, Irene Farabella, Mike N. Goodstadt, and Marc A. Marti-Renom

5. Targeted DNase Hi-C

            Zhijun Duan

6. C-HiC: A High Resolution Method for Unbiased Chromatin Conformation Capture Targeting Small Locus

            Jérôme D. Robin

7. Computational Analysis of Hi-C Data

            Mattia Forcato and Silvio Bicciato

            Xue Qing David Wang, Christopher J.F. Cameron, Dana Segal, Denis Paquette, Mathieu Blanchette, and Josée Dostie

9. Single-Cell DamID to Capture Contacts between DNA and the Nuclear Lamina in Individual Mammalian Cells

            Kim L. de Luca and Jop Kind

10. Formaldehyde-Mediated Snapshot of Nuclear Architecture

            Federica Lucini, Andrea Bianchi, and Chiara Lanzuolo

11. Visualization of Chromatin Dynamics by Live Cell Microscopy Using CRISPR/Cas9 Gene Editing and ANCHOR Labeling

            Ezio T. Fok, Stephanie Fanucchi, Kerstin Bystricky, and Musa M. Mhlanga

12. Preparing Map of Chromosome Territories Distribution Frequency

            Paulina Nastały and Paolo Maiuri

13. Higher-Order Chromatin Organization Using 3D DNA Fluorescent In Situ Hybridization

            Quentin Szabo, Giacomo Cavalli, and Frédéric Bantignies

14. 3D Immuno-DNA Fluorescence In Situ Hybridization (FISH) for Detection of HIV-1 and Cellular Genes in Primary CD4⁺ T Cells

            Bojana Lucic, Julia Wegner, Mia Stanic, Katharina Laurence Jost, and Marina Lusic

15. Visualization of Nuclear and Cytoplasmic Long Noncoding RNAs at Single-Cell Level by RNA-FISH

            Tiziana Santini, Julie Martone, and Monica Ballarino

16. 3D COMBO chrRNA-DNA-Immuno FISH

            Federica Marasca, Alice Cortesi, and Beatrice Bodega

17. An Algorithm for the Analysis of the 3D Spatial Organization of the Genome

            Francesco Gregoretti, Alice Cortesi, Gennaro Oliva, Beatrice Bodega, and Laura Antonelli

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts