Plant Phosphoproteomics, 2015 Methods and Protocols Methods in Molecular Biology Series, Vol. 1306
Coordonnateur : Schulze Waltraud X.
This detailed volume addresses recent developments in phosphoproteomic techniques with a particular focus on the plant system. Over the recent decades, proteomic methods were refined to study the significance and dynamics of protein phosphorylation in various biological contexts. However, working with plant tissue imposes particular challenges to the biologist which are attributed to the rigid cell wall making protein extraction more difficult, the skewed protein abundance with Rubisco as a highly abundant protein and a large central vacuole leading to low protein yield and increased degradative enzyme activity. The methodologies in this book seek to move beyond these issues. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Practical and authoritative, Plant Phosphoproteomics: Methods and Protocols serves as an ideal reference for researchers investigating this vital area of plant science.
1. The Plant Kinome
Monika Zulawski and Waltraud X. Schulze
2. Phosphatases in Plants
Alois Schweighofer and Irute Meskiene
3. Phosphoproteomics in Cereals
Pingfang Yang
4. Screening of Kinase Substrates Using Kinase-Knockout Mutants
Taishi Umezawa
5. Phosphopeptide Profiling of Receptor Kinase Mutants
Xu Na Wu and Waltraud X. Schulze
6. Combining Metabolic 15N Labelling with Improved TandemMOAC for Enhanced Probing of the Phosphoproteome
Martin Thomas, Wolfgang Hoehenwarter, Uwe Conrath, and Gerold J.M. Beckers
7. Kinase Activity and Specificity Assay Using Synthetic Peptides
Xu Na Wu and Waltraud X. Schulze
8. Absolute Quantitation of Protein Post-Translational Modification Isoform
Zhu Yang and Ning Li
9. Phosphorylation Stoichiometry Determination in Plant Photosynthetic Membranes
Björn Ingelsson, Rikard Fristedt, and Maria V. Turkina
10. Phosphopeptide Immuno-Affinity Enrichment to Enhance Detection of Tyrosine Phosphorylation in Plants
Sharon C. Mithoe and Frank L.H. Menke
11. The Peptide Microarray ChloroPhos1.0: A Screening Tool for the Identification of Arabidopsis thaliana Chloroplast Protein Kinase Substrates
Anna Schönberg and Sacha Baginsky
12. Plant Protein Kinase Substrates Identification Using Protein Microarrays
Shisong Ma and Savithramma P. Dinesh-Kumar
13. Targeted Analysis of Protein Phosphorylation by 2D Electrophoresis
Kristin Mayer, Sally Albrecht, and Andreas Schaller
14. Computational Phosphorylation Network Reconstruction: Methods and Resources
Guangyou Duan and Dirk Walther
15. Computational Identification of Protein Kinases and Kinase-Specific Substrates in Plants
Han Cheng, Yongbo Wang, Zexian Liu, and Yu Xue
16. Databases for Plant Phosphoproteomics
Waltraud X. Schulze, Qiuming Yao, and Dong Xu
17. Phosphorylation Site Prediction in Plants
Qiuming Yao, Waltraud X. Schulze, and Dong Xu
Includes cutting-edge methods and protocols in plant phosphoproteomics
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Ouvrage de 231 p.
17.8x25.4 cm
Ouvrage de 231 p.
17.8x25.4 cm