Single Cell Protein Analysis, Softcover reprint of the original 1st ed. 2015 Methods and Protocols Methods in Molecular Biology Series, Vol. 1346

This volume highlights recent developments in flow cytometry, affinity assays, imaging, mass spectrometry, microfluidics and other technologies that enable analysis of proteins at the single cell level. The book also includes chapters covering a suite of biochemical and biophysical methods capable of making an entire gamut of proteomic measurements, including analysis of protein abundance or expression, protein interaction networks, post-translational modifications, translocation and enzymatic activity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Single Cell Protein Analysis: Methods and Protocols is useful to researchers and students in biological and biomedical sciences who have an interest in proteomic measurements in cells. 

1. Single-Cell Western Blotting
Elly Sinkala and Amy E. Herr

2. A Microfluidic Device for Immunoassay-Based Protein Analysis of Single E. Coli Bacteria
Simone Stratz and Petra S. Dittrich

3. Enzyme-Linked ImmunoSpot (ELISpot) for Single Cell Analysis
Sylvia Janetzki and Rachel Rabin

 4. Photocleavable DNA Barcoding Antibodies for Multiplexed Protein Analysis in Single Cells
Adeeti V. Ullal and Ralph Weissleder

 5. Genome-Wide Analysis of Protein and mRNA Copy Numbers in Single Escherichia Coli Cells with Single-Molecule Sensitivity
Yuichi Taniguchi

6. Microfluidic Flow Cytometry for Single Cell Protein Analysis
Meiye Wu and Anup K. Singh

7. Microfluidic Image Cytometry for Single Cell Phenotyping of Human Pluripotent Stem Cells
Yasumasa Mashimo and Ken-Ichiro Kamei

8. Characterizing Phenotypes and Signaling Networks of Single Human Cells by Mass Cytometry
Nalin Leelatian, Kirsten E. Diggins, and Jonathan M. Irish

9. Multiplex Peptide-MHC Tetramer Staining with Mass Cytometry
Leong Mei Ling and Evan W. Newell

10. Imaging and Mapping of Tissue Constituents at the Single Cell Level Using MALDI MSI and Quantitative Laser Scanning Cytometry
Catherine M. Rawlins, Joseph P. Salisbury, Daniel R. Feldman, Sinan Isim, Nathalie Y.R. Agar, Ed Luther, and Jeffery N. Agar

 11. SPLIFF: A Single Cell Method to Map Protein-Protein Interactions in Time and Space
Alexander Dünkler, Reinhild Rösler, Hans A. Kestler, Daniel Moreno, and Nils Johnsson

12. Microfluidic Proximity Ligation Assay for Profiling Signaling

13. Dynamics and Interactions of Individual Proteins in the Membrane of Single, Living Cells
Stephen Anthony, Amanda Carroll-Portillo, and Jerilyn Timlin

14. Microfluidics Enabled Enzyme Activity Measurement in Single Cells
Cinzia Tesauro, Rikke Frøhlich, Magnus Stougaard, Yi-Ping Ho, and Birgitta R. Knudsen

15. Microfluidic Chemical Cytometry for Enzyme Assays of Single Cells
Livia Shehaj, Lorena Lazo de la Vega, and Michelle L. Kovarik

16. Quantitative Detection of Nucleocytoplasmic Transport of Native Proteins in Single Cells
Zhenning Cao and Chang Lu

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras