Neural Progenitor Cells, 2013 Methods and Protocols Methods in Molecular Biology Series, Vol. 1059
Coordonnateurs : Reynolds Brent A., Deleyrolle Loic P.
The discovery of stem and progenitor cells in the adult mammalian CNS challenged the long standing ?no new neuron? doctrine and opened the door to the potential for cell replacement therapy. The process from discovery to clinical applications can be long and tortuous, requiring rigorous steps involving standardized and precise protocols. Neural Progenitor Cells: Methods and Protocols is a collection of practical articles describing techniques used to study neural stem and progenitor cells. The volume also highlights the promise of stem cell-based therapeutic applications for CNS disorders. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Neural Progenitor Cells: Methods and Protocols serves both professionals and novices with its well-honed methodologies.
Part I- Culturing Neural Precursor Cells from Primary Tissue
1. Culturing Fetal Precursors Cells Using Free-Floating Serum-Free Conditions
Andrew Chojnacki and Samuel Weiss
2. In vitro Expansion of Foetal Neural Progenitors as Adherent Cell Lines
Steven M. Pollard
3. Isolate and Culture Precursor Cells from the Adult Periventricular Area
Chiara Cavazzin, Margherita Neri, and Angela Gritti
4. Culturing and Expansion of Precursor Cells from the Adult Hippocampus
Dhanisha J. Jhaveri, Boris W. Prosper, and Perry F. Bartlett
5. Isolate and Culture Neural Stem Cells from the Mouse Adult Spinal Cord
Jean-Philippe Hugnot
6. Culturing and Expansion of “Clinical Grade” Precursors Cells from the Foetal Human Central Nervous System
Maurizio Gelati, Daniela Profico, Massimo Projetti-Pensi, Gianmarco Muzi, Giada Sgaravizzi, and Angelo Luigi Vescovie
7. Isolating and Culturing of Precursor Cells from the Adult Human Brain
Florian A. Siebzehnrubl and Dennis A. Steindler
8. Isolation and Culture of Precursor Cells from the Adult Human Spinal Cord
Luc Bauchet, Nicolas Lonjon, Florence Vachiery-Lahave, Alain Boularan, Alain Privat, and Jean-Philippe Hugnot
9. Isolation and Enrichment of Defined Neural Cell Populations from Heterogeneous Neural Stem Cell Progeny
Hassan Azari
10. Isolation of Adult Stem Cells from the Human Olfactory Mucosa
François Féron, Chris Perry, Stéphane D. Girard, and Alan Mackay-Sim
Part II. Stem Cells vs Progenitor Cells
11. Enumerating Stem Cell Frequency – Neural Colony Forming Cell Assay
Sharon A. Louis and Carmen K. H. Mak
Part III. Purification of Stem and Progenitor Cells
12. Flow Cytometry of Neural Cells
Geoffrey W. Osborne
Part IV. Transplantation of Neural Progenitor Cells: Preclinical models
13. Neonatal Transplant in Hypoxic Injury
Tong Zheng and Michael D. Weiss
14. Isolation and Purification of Self Renewable Human Neural Stem Cells for Cell Therapy in Experimental Model of Ischemic Stroke
Ricardo L. Azevedo-Pereira and Marcel M. Daadi
15. Transplantation of Foetal Midbrain Dopamine Progenitors into a Rodent Model of Parkinson’s Disease
Lachlan H. Thompson and Clare L. Parish
Part V. In vivo Identification of Neural Precursor Cells
16. Distribution of Neural Precursor Cells in Rodent Brain
Daniel G. Blackmore and Rodney L. Rietze
17. Identifying Neural Precursor Cells in the Adult Human Brain
Thomas I.H. Park, Henry J. Waldvogel, Johanna M. Montgomery, Edward W. Mee, Peter S. Bergin, Richard L.M. Faull, Mike Dragunow, and Maurice A. Curtis
Part VI. Clinical Application of Neural Precursor Cell Therapy
18. Clinical Trials for the Treatment of Spinal Cord Injury: Not So Simple
Alan Mackay-Sim and François Féron
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
Date de parution : 08-2016
Ouvrage de 241 p.
17.8x25.4 cm
Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).
Prix indicatif 110,76 €
Ajouter au panierDate de parution : 08-2013
Ouvrage de 241 p.
17.8x25.4 cm
Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).
Prix indicatif 105,49 €
Ajouter au panierThèmes de Neural Progenitor Cells :
Mots-clés :
cell cultures; neural precursor cell; neural progenitor cell; progenitor cell; stem cell