Urothelial Carcinoma, 1st ed. 2018 Methods and Protocols Methods in Molecular Biology Series, Vol. 1655

This volume details standard techniques for the characterization of urothelial carcinoma as well as methods to investigate mechanisms of carcinogenesis.  Chapters guide readers on cellular and animal models for urothelial carcinoma and related diseases, molecular analyses from body fluids, and new approaches to therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Urothelial Carcinoma: Methods and Protocols hopes that the techniques described in this volume will contribute to the current upturn in research on urothelial carcinoma and to the application of its results in clinical practice.

PART I: Molecular Characterization

1.      Analysis of Chromosomal Alterations in Urothelial Carcinoma
Donatella Conconi and Angela Bentivegna

2.      Analysis of Point Mutations in Clinical Samples of Urothelial Carcinoma
Mustafa Alamyar and Ellen C. Zwarthoff

3.      A Versatile Assay for Detection of Aberrant DNA Methylation in Bladder Cancer
Stella Tommasi and Ahmad Besaratinia

4.      Immunohistochemical Analysis of UC Tissues for Proliferation and Differentiation Markers
Michael Rose and Nadine Therese Gaisa

5.      Molecular Subtype Profiling of Urothelial Carcinoma Using a Subtype-Specific Immunohistochemistry Panel
Gottfrid Sjödahl

PART II:  Urothelial Carcinogenesis

6.      Defining the Pathways of Urogenital Schistosomiasis-associated Urothelial Carcinogenesis through Transgenic and Bladder Wall Egg Injection Models
Evaristus C. Mbanefo and Michael H. Hsieh

7.      Algorithm for the Automated Evaluation of NAT2 Genotypes
Georg Michael, Ricarda Thier, Meinolf Blaszkewicz, Silvia Selinski, and Klaus Golka

8.      Detection of APOBEC3 Proteins and Catalytic Activity in Urothelial Carcinoma
Ananda Ayyappan Jaguva Vasudevan, Wolfgang Göring, Dieter Häussinger, and Carsten Münk

9.      Oxidative Stress in Urothelial Carcinogenesis: Measurements of Protein Carbonylation and Intracellular Production of Reactive Oxygen Species
Patcharawalai Whongsiri, Suchittra Phoyen, and Chanchai Boonla

PART III: Cellular and ANIMAL MODELS

10.  Urothelial Carcinoma Stem Cells - current Concepts, Controversies, and Methods
Jiri Hatina, Hamendra Singh Parmar, Michaela Kripnerova, Anastasia Hepburn, and Rakesh Heer

11. 

12.  Spheroid Cultures of Primary Urothelial Cancer Cells: Cancer Tissue-originated Spheroid (CTOS) Method
Takahiro Yoshida, Hiroaki Okuyama, Hiroko Endo, and Masahiro lnoue

13.  The N-butyl-N-4-hydroxybutyl Nitrosamine Mouse Urinary Bladder Cancer Model
Paula Alexandra Oliveira, Cármen Vasconcelos-Nóbrega, Rui Manuel Gil da Costa,  and Regina Arantes-Rodrigues

14.  Patient-derived Bladder Cancer Xenografts
Carina Bernardo and Lúcio Lara Santos

15.<  Orthotopic Mouse Models of Urothelial Cancer
Wolfgang Jäger, Igor Moskalev, Peter Raven, Akihiro Goriki, Samir Bidnur, and Peter C. Black

PART IV: Biomarkers

20.  Isolation and Characterization of CTCs from Patients with Cancer of a Urothelial Origin
Vladimir Bobek and Katarina Kolostova

PART V: Therapy Development

21.  Epigenetic Treatment Options in Urothelial Carcinoma
Guenter Niegisch, Maria Pinkerneil, and Michèle Hoffmann

22.  Evaluation of Protein Levels of the Receptor Tyrosine Kinase ErbB3 in Serum
Leandro S. D'Abronzo, Chong-Xian Pan, and Paramita M. Ghosh

23.  Targeting the PI3K/AKT/mTOR Pathway in Bladder Cancer
Anuja Sathe and Roman Nawroth

24.  Visualization and Quantitative Measurement of Frug-induced Platinum Sdducts in the Nuclear DNA of Individual Cells by an Immuno-cytological Sssay
Margarita Melnikova and Jürgen Thomale

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras