Esophageal Squamous Cell Carcinoma, 1st ed. 2020 Methods and Protocols Methods in Molecular Biology Series, Vol. 2129

This volume provides current management and research protocols on oesophageal squamous cell carcinoma. Chapters guide readers through methods on clinical and pathological diagnostics, translational research for oesophageal squamous cell carcinoma, in vitro assays, liquid biopsy for cancer DNA and circulating tumour cells, genomic analysis, mi-RNAs, and proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Esophageal Squamous Cell Carcinoma: Methods and Protocols to ensure successful results in the further study of this vital field.

1. Introduction: Oesophageal Squamous Cell Carcinoma:  Current Status and Future Advances

Alfred K. Lam

Alfred K. Lam

3. Application of Pathological Staging in Oesophageal Squamous Cell Carcinoma

Alfred K. Lam

4. Macroscopic Examination of Surgical Specimen of Oesophageal Squamous Cell Carcinoma

Alfred K. Lam

5. Endoscopic Diagnosis and Treatment of Oesophageal Squamous Cell Carcinoma

6. Macroscopic Assessment and Sampling of Endoscopic Resection Specimens for Squamous Epithelial Malignancies with Superficial Involvement of Oesophagus

7. Roles of Pathological Assessments of Frozen Sections in Oesophageal Squamous Cell Carcinoma

Alfred K. Lam

8. Biobanking for Oesophageal Squamous Cell Carcinoma

Alfred K. Lam

9. Whole-slide Imaging of Oesophageal Squamous Cell Carcinoma

Alfred K. Lamand Melissa Leung

10. Use of Tissue Microarray in Oesophageal Squamous Cell Carcinoma

Alfred K. Lamand David K. Lor

11. Patient-derived Xenograft and Mice Models in Oesophageal Squamous Cell Carcinoma

Alfred K. Lamand Johnny C. Tang

Valen Z. Yu, Joseph C. Y.  Ip, Josephine M. Y.  Ko, Lihua Tao, Alfred K. Lam, and Maria L. Lung

13. In Vitro Assays of Biological Aggressiveness of Oesophageal Squamous Cell Carcinoma

Farhadul Islam, Vinod Gopalan, and Alfred K. Lam

14. Detention and Identification of Cancer Stem Cells in Oesophageal Squamous Cell Carcinoma

Farhadul Islam, Vinod Gopalan, and Alfred K. Lam

15. Liquid Biopsy: Detection of Circulating Tumour Cells in Oesophageal Squamous Cell Carcinoma

Alfred K. Lam,   Faysal Bin Hamid, and Vinod Gopalan

16. Liquid Biopsy for Investigation of Cancer DNA in Oesophageal Squamous Cell Carcinoma

Robert A. Smith and Alfred K. Lam

17. Genome Sequencing in Oesophageal Squamous Cell Carcinoma

Suja Pillai, Neven Maksemous, and Alfred K. Lam

18. Roles of microRNAs in Oesophageal Squamous Cell Carcinoma Pathogenesis

Farhadul Islam, Vinod Gopalan, Alfred K. Lam

19. Mass-spectrometry for Biomarkers Discovery in Oesophageal Squamous Cell Carcinoma

Farhadul Islam, Vinod Gopalan, and Alfred K. Lam

20. Immunoblotting in Detection of Tumour-associate Antigens in Oesophageal Squamous Cell Carcinoma

Farhadul Islam, Vinod Gopalan, and Alfred K. Lam

21. Immunohistochemistry for Protein Detection in Oesophageal Squamous Cell Carcinoma

Kais Kasemand Alfred K. Lam

22. Radiotherapy for Cervical Oesophageal Squamous Cell Carcinoma

Dora L.W. Kwong, Wendy W.L.Chan, and Ka On Lam

23. Radiotherapy for Thoracic Oesophageal Squamous Cell Carcinoma

24. Systemic Therapy for Oesophageal Squamous Cell Carcinoma

Ka On Lam, Wendy W.L. Chan, Tsz Him So, and Dora L.W. Kwong

25. Surgical Protocols for Squamous Cell Cancer of the Oesophagus

26. Anaesthetic Management for Squamous Cell Carcinoma of the Oesophagus

Eva Y. F. Chan, Danny  K. Y. Ip, and Michael G. Irwin

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts